5 research outputs found
SDS-PAGE and native PAGE analysis of control and USP laser-treated plasma proteins.
<p>(A) On the left is shown the SDS-PAGE of control and laser-treated plasma; on the right, for comparison, is shown the SDS-PAGE of laser-treated MCMV virus adapted from Tsen <i>et al</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111673#pone.0111673-Tsen3" target="_blank">[13]</a> (reprinted with permission from the Society of Photo-Optical Instrumentation Engineers). Control (untreated) or USP laser-treated plasma were boiled in reducing buffer and separated on a 10% gel. Proteins were visualized by Coomassie blue stain. The solid arrow indicates the location of low mobility detergent-resistant aggregates; the dotted arrow indicates missing band(s) corresponding to aggregated proteins. (B) Native PAGE of control and laser-treated plasma. Control (untreated) or USP laser-treated plasma were separated on a 10% gel. Proteins were visualized by Coomassie blue stain. Arrows indicate location of low mobility detergent-resistant aggregates.</p
Dynamic light scattering measurements on the aggregation state of fibrinogen.
<p>Dynamic light scattering measurements on the aggregation state of fibrinogen.</p
Structural analysis of control and laser-treated fibrinogen protein.
<p>Control (untreated) or laser-treated fibrinogen protein was analyzed by circular dichroism. The red line indicates the CD spectrum of control fibrinogen, while the dotted black line indicates laser treated fibrinogen. The spectra (in Mean Residue Ellipticity, rescaled for concentration) show virtually complete overlap.</p
Inactivation of viruses in plasma using a USP laser.
<p>Human plasma containing HIV (A), HAV (B), or MCMV (C) were treated with the USP laser. For the HIV-spiked plasma, viral titer was assessed by plaque assay in MAGI cells. For the HAV-spiked plasma, viral titer was assessed by plaque assay in fetal rhesus monkey kidney cells. For the MCMV-spiked plasma, viral titer was assessed by TCID<sub>50</sub> assay in murine embryonic fibroblast cells. Results are representative of triplicate experiments and are shown as means ± SEM.</p
Retention of coagulation factor activity for USP laser-treated plasma.
<p>Retention of coagulation factor activity for USP laser-treated plasma.</p