Influence of CLL18 on chemoresistance.

<p>Cells were cultured in presence or absence of -TGFbeta or CCL18 in the concentrations indicated for 72 h. After the culture period, medium was changed and the cells were incubated with cisplatin for additional 72 hours at concentrations ranging from 0.4 to 25 µg/ml. Cell survival was measured by MTT assay. The horizontal line indicates the LD₅₀, vertical lines indicate the LD₅₀ for the respective pre-incubation condition (n = 4).</p

Mean CCL18 levels of all T stages was significantly increased compared with controls (p<0.0002).

<p>In addition, there was a significant difference between patient groups with lowest versus the two highest T-stages.</p

CCL18 induces chemotaxis in A549 lung cancer cells.

<p>Significances were calculated using paired comparison (Wilcoxon Signed Rank Test), control: untreated cells (n = 4).</p

Summary of clinico-pathological data of included NSCLC patients.

<p>Summary of clinico-pathological data of included NSCLC patients.</p

Immunohistological images obtained from multi-antibody assay compared to single antibody stains.

<p>Normal lung tissue (A), LAC (B), SCC (C) and LCNEC (D) stained with the combined multi-antibody assay (top) and each single included antibody. Red nuclei – TTF1; red cytoplasm – vimentin; brown nuclei – p63; brown cytoplasm – neuroendocrine markers. Below the bi-color IHC assay corresponding single antibody assays are displayed in the same tumor area. Comparison of each single antibody staining and the bi-color multi antibody assay shows analog specific positivity in the same cells and does not reveal any unspecific background staining or chromogenic cross reactivity.</p