Skin carotenoid status measured by resonance Raman spectroscopy as a biomarker of fruit and vegetable intake in preschool children

pre-printBackground/Objective: Dietary assessment in children is difficult, suggesting a need to develop more objective biomarkers of intake. Resonance Raman spectroscopy (RRS) is a noninvasive, validated method of measuring carotenoid status in skin as a biomarker of fruit/vegetable intake. The purpose of this study was to examine the feasibility of using RRS in preschool children, including describing the inter-individual variability in skin carotenoid status and to identify factors associated with the biomarker in this population. Subjects/Methods: We conducted a cross-sectional study of 381 economically-disadvantaged preschoolers in urban centers in Connecticut (U.S.). 85.5% were black non-Hispanic or Hispanic/Latino, and 14.1% were obese and 16.9% were overweight by age- and sex-specific BMI percentiles. Children had their skin carotenoid status assessed by RRS in the palm of the hand. Fruit/vegetable consumption was assessed by a brief parent/guardian-completed food frequency screener and a liking survey. Results: We observed inter-individual variation in RRS values that was nearly normally distributed. In multiple regression analysis, higher carotenoid status, measured by RRS, was positively associated with fruit/vegetable consumption (p=0.02) and fruit/vegetable preference (p<0.01). Lower carotenoid status was observed among younger children, those participating in the U.S. Supplemental Nutrition Assistance Program (SNAP), and those with greater adiposity (p<0.05 for all). Conclusions: We observed wide variability in skin carotenoid status in a population of young children, as assessed by RRS. Parent-reported fruit/vegetable intake and several demographic factors were significantly associated with RRS-measured skin carotenoid status. We recommend further development of this biomarker in children, including evaluating response to controlled interventions

Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits

Our understanding of neural circuits--how they mediate the computations that subserve sensation, thought, emotion, and action, and how they are corrupted in neurological and psychiatric disorders--would be greatly facilitated by a technology for rapidly targeting genes to complex 3-dimensional neural circuits, enabling fast creation of "circuit-level transgenics." We have recently developed methods in which viruses encoding for light-sensitive proteins can sensitize specific cell types to millisecond-timescale activation and silencing in the intact brain. We here present the design and implementation of an injector array capable of delivering viruses (or other fluids) to dozens of defined points within the 3-dimensional structure of the brain (Figure. 1A, 1B). The injector array comprises one or more displacement pumps that each drive a set of syringes, each of which feeds into a polyimide/fused-silica capillary via a high-pressure-tolerant connector. The capillaries are sized, and then inserted into, desired locations specified by custom-milling a stereotactic positioning board, thus allowing viruses or other reagents to be delivered to the desired set of brain regions. To use the device, the surgeon first fills the fluidic subsystem entirely with oil, backfills the capillaries with the virus, inserts the device into the brain, and infuses reagents slowly (<0.1 microliters/min). The parallel nature of the injector array facilitates rapid, accurate, and robust labeling of entire neural circuits with viral payloads such as optical sensitizers to enable light-activation and silencing of defined brain circuits. Along with other technologies, such as optical fiber arrays for light delivery to desired sets of brain regions, we hope to create a toolbox that enables the systematic probing of causal neural functions in the intact brain. This technology may not only open up such systematic approaches to circuit-focused neuroscience in mammals, and facilitate labeling of brain regions in large animals such as non-human primates, but may also open up a clinical translational path for cell-specific optical control prosthetics, whose precision may enable improved treatment of intractable brain disorders. Finally, such devices as described here may facilitate precisely-timed fluidic delivery of other payloads, such as stem cells and pharmacological agents, to 3-dimensional structures, in an easily user-customizable fashion.National Institutes of Health (U.S.) (NIH Director's New Innovator Award (DP2 OD002002-01)National Institutes of Health (U.S.) (NIH Challenge Grant 1RC1MH088182-01)National Institutes of Health (U.S.) (NIH Grand Opportunities Grant 1RC2DE020919-01)National Institutes of Health (U.S.) (NIH Grand Opportunities Grant NIH 1R01NS067199-01)National Science Foundation (U.S.) (NSF 0848804)National Science Foundation (U.S.) (NSF 0835878)McGovern Institute for Brain Research at MIT (Neurotechnology Award Program)National Alliance for Research on Schizophrenia and Depression (U.S.)Alfred P. Sloan FoundationDr. Gerald Burnett and Marjorie BurnettUnited States. Dept. of DefenseSociety for Neuroscience (SFN Research Award for Innovation in Neuroscience)Massachusetts Institute of Technology. Media LaboratoryBenesse FoundationWallace H. Coulter Foundatio

SuperNova Acceleration Probe (SNAP): Investigating Photometric Redshift Optimization

The aim of this paper is to investigate ways to optimize the accuracy of photometric redshifts for a SNAP like mission. We focus on how the accuracy of the photometric redshifts depends on the magnitude limit and signal-to-noise ratio, wave-length coverage, number of filters and their shapes and observed galaxy type. We use simulated galaxy catalogs constructed to reproduce observed galaxy luminosity functions from GOODS, and derive photometric redshifts using a template fitting method. By using a catalog that resembles real data, we can estimate the expected number density of galaxies for which photometric redshifts can be derived. We find that the accuracy of photometric redshifts is strongly dependent on the signal-to-noise (S/N) (i.e., S/N>10 is needed for accurate photometric redshifts). The accuracy of the photometric redshifts is also dependent on galaxy type, with smaller scatter for earlier type galaxies. Comparing results using different filter sets, we find that including the U-band is important for decreasing the fraction of outliers, i.e., ``catastrophic failures''. Using broad overlapping filters with resolution ~4gives better photometric redshifts compared to narrower filters (resolution >~5) with the same integration time. We find that filters with square response curves result in a slightly higher scatter, mainly due to a higher fraction of outliers at faint magnitudes. We also compare a 9-filter set to a 17-filter set, where we assume that the available exposure time per filter in the latter set is half that of the first set. We find that the 9-filter set gives more accurate redshifts for a larger number of objects and reaches higher redshift, while the 17-filter set is gives better results at bright magnitudes.Comment: 30 pages, 10 figures. Submitted to A

Prospectus, December 8, 1999

https://spark.parkland.edu/prospectus_1999/1033/thumbnail.jp

Prospectus, February 9, 2000

https://spark.parkland.edu/prospectus_2000/1004/thumbnail.jp

Should Postponing Motherhood via “Social Freezing” Be Legally Banned? An Ethical Analysis

In industrial societies, women increasingly postpone motherhood. While men do not fear a loss of fertility with age, women face the biological boundary of menopause. The freezing of unfertilized eggs can overcome this biological barrier. Due to technical improvements in vitrification, so-called “social freezing” (SF) for healthy women is likely to develop into clinical routine. Controversial ethical debates focus on the risks of the technique for mother and child, the scope of reproductive autonomy, and the medicalization of reproduction. Some criticize the use of the technique in healthy women in general, while others support a legally defined maximum age for women at the time of an embryo transfer after oocyte cryopreservation. Since this represents a serious encroachment on the reproductive autonomy of the affected women, the reasons for and against must be carefully examined. We analyze arguments for and against SF from a gendered ethical perspective. We show that the risk of the cryopreservation of oocytes for mother and future child is minimal and that the autonomy of the women involved is not compromised. The negative ethical evaluation of postponed motherhood is partly due to a biased approach highlighting only the medical risks for the female body without recognizing the potential positive effects for the women involved. In critical accounts, age is associated in an undifferentiated way with morbidity and psychological instability and is thus used in a discriminatory way. We come to the conclusion that age as a predictor of risk in the debate about SF is, from an ethical point of view, an empty concept based on gender stereotypes and discriminatory connotations of aging. A ban on postponing motherhood via SF is not justified

Parental Language Input to Children at Stuttering Onset

Many programs for the indirect management of stuttering in early childhood counsel adjustment of parental language models, which are presumed to play an exacerbating influence on vulnerable children’s fluency. We examined the relative levels of linguistic demand in maternal language to stuttering and nonstuttering children, adjusted for each child’s current level of linguistic development. No significant or observable differences were detected in the relative level of linguistic demand posed by parents of stuttering children very close to onset of symptoms. Empirical support for current advisement and potential ramifications are discussed

Prospectus, March 29, 2000

https://spark.parkland.edu/prospectus_2000/1010/thumbnail.jp