Effects of the storage of decontamination reagents on the DNA decontamination efficacy of the undiluted reagents.

<p>Effects of the storage of decontamination reagents on the DNA decontamination efficacy of the undiluted reagents.</p

Evaluation of the nucleic acid decontamination efficacy by the surface test protocol.

<p>Evaluation of the nucleic acid decontamination efficacy by the surface test protocol.</p

Evaluation of the nucleic acid decontamination efficacy by the solution test protocol.

<p>Evaluation of the nucleic acid decontamination efficacy by the solution test protocol.</p

Set of decontamination reagents applied in this study.

<p>Set of decontamination reagents applied in this study.</p

Summary of calves borne in both farms in North Rhine-Westphalia, Germany.

<p>The first number indicates the total number of calves in the respective group, inside the parentheses the number of calves is given separately for both holdings.</p

Oligonucleotide sequences and composition of primer-probe mixes used in this study.

<p>Oligonucleotide sequences and composition of primer-probe mixes used in this study.</p

Additional file 1: of Influence of cell type and cell culture media on the propagation of foot-and-mouth disease virus with regard to vaccine quality

Table S1.1. Nucleotide changes in the VP1 coding region of FMDV type O1 Manisa during serial passaging in adherent BHK21 cells. Table S1.2. Nucleotide changes in the VP1 coding region of FMDV type O1 Manisa during serial passaging in BHK-2P suspension cells. Table S1.3. Nucleotide changes in the capsid-coding region of FMDV type A24 Cruzeiro during serial passaging in adherent BHK21 cells. Table S1.4. Nucleotide changes in the capsid-coding region of FMDV type A24 Cruzeiro during serial passaging in BHK-2P suspension cells. (DOCX 17 kb

Detailed analyses of newborn calves in 2 farms in North Rhine-Westphalia.

<p>Meconium samples of calves whose ELISA results are marked in red tested positive by real-time RT-PCR; blue ELISA results correspond to negative PCR results. The day of gestation at the estimated time of SBV-infection of the respective cow is symbolized by a light blue area.</p

Additional file 3: of Influence of cell type and cell culture media on the propagation of foot-and-mouth disease virus with regard to vaccine quality

Table S3. Summary of virus isolates, cell culture systems and mutations acquired during passaging. (DOCX 13 kb

Additional file 2: of Influence of cell type and cell culture media on the propagation of foot-and-mouth disease virus with regard to vaccine quality

Table S2. In-silico analysis covering possible additional vaccine strains (A) and most topotypes within the different serotypes (B) of FMDV. (DOCX 35 kb