MOESM1 of Oral vaccination of wildlife using a vaccinia–rabies-glycoprotein recombinant virus vaccine (RABORAL V-RG®): a global review

Additional file 1. Safety testing of V-RG in non-target species. This table contains compiled information summarizing studies of RABORAL V-RG vaccine safety in a diversity of animal species that are not the primary target for vaccination (non-target) but could be inadvertently exposed to the vaccine through environmental release

MOESM2 of Oral vaccination of wildlife using a vaccinia–rabies-glycoprotein recombinant virus vaccine (RABORAL V-RG®): a global review

Additional file 2. Efficacy studies using V-RG in current or potential primary target species administered by various routes. This table contains compiled information summarizing studies of RABORAL V-RG immunogenicity and efficacy in wildlife species that are the current target of label or experimental use of this vaccine or have been identified as possible future primary targets for oral vaccination to support rabies control and prevention efforts

Comparative analysis of viral genome detection via real-time RT-PCR. Mean C_q values from duplicate runs.

<p>RABV: <i>Rabies virus</i>; ;EBLV: <i>European Bat Lyssavirus</i>;neg.: negative control; −: negative result; #: cross-reactivitiy with other <i>Lyssavirus</i> species; ?: doubtful result;</p>*<p>doubtful result was retested; i.h.: in-house assay; dilution series (0), (I), (II), (III); 10⁰, 10⁻¹, 10⁻², 10⁻³; mod.: assay modified; r: RABV-specific detection; e1: EBLV-1 specific; e1+2: EBLV-1+−2 specific; r13, r14, r13/14: R13, R14, duplex R13/14 assay by <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058372#pone.0058372-Hoffmann1" target="_blank">[17]</a>; <i>fn</i>: false negative results; <b>exp</b>: expected negative results from previous publication; ts: two-step systems; no duplicates for assays D2, D3 and M; 2) Orlowska et al., 2008 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058372#pone.0058372-Orlowska1" target="_blank">[22]</a>.</p

Mean quantification cycle (C_q) values of viral genome detection via EBLV-1 and EBLV-2 specific real-time RT-PCR.

<p>RABV: <i>Rabies virus</i>;;EBLV: <i>European Bat Lyssavirus</i>;neg.: negative control; –: not applicable; #: cross–reactivitiy with other <i>Lyssavirus</i> species; ?: inconclusive based on curve shape; dilution series (0), (I), (II), (III); 10⁰, 10⁻¹, 10⁻², 10⁻³;</p>*<p>Hoffmann and Müller personal communication; ts: two-step systems; no duplicates for assays M1 and M2; all laboratories except J1 used separate species-specific real-time PCRs to detect EBLV-1 or -2.</p