Towards a cost-efficient & standardised monitoring protocol for sub-tidal reef fish in the Agulhas eco-region of South Africa

Under the growing demand for marine fish resources, and the apparent and expected impacts of global climate change, there is a need to conduct long-term monitoring (LTM) to ensure effective management of resources and conservation of biodiversity. However LTM programmes often suffer from design deficiencies and fail to achieve their objectives. These deficiencies stem from the fact that insufficient consideration is afforded to the design phase, with programmes selecting methods that are not suitable to address the objectives, or are not cost-efficient, compromising the sustainability of the LTM. To facilitate the establishment of LTM programmes along the southern coast of South Africa, background research needed to be conducted to identify which methods were most appropriate for LTM of reef fish. This study presents a detailed field-based assessment of the suitability and cost-efficiency of monitoring methods for long-term monitoring of reef fish in the Agulhas Ecoregion of South Africa. The approach adopted to identify the method, or suite of methods most suited for LTM, involved (i) the selection of methods considered suitable for LTM, (ii) the individual assessment and optimisation of method performance, and (iii) the comparative assessment of the fish community sampled by the different methods. The most suited method(s) were then identified as those that provide the most comprehensive assessment of the fish community and had the highest cost-efficiency. The research was conducted between January 2008 and 2011 in the Tsitsikamma and Table Mountain National Park (TNP and TMNP, respectively) marine protected areas (MPAs) within the Agulhas Ecoregion. The methods selected included fish traps (FT), controlled angling (CA), underwater visual census (UVC), remote underwater video (RUV), baited RUV (BRUV) and remotely operated vehicles (ROV). The individual assessment and optimisation was conducted with the FT, UVC, RUV and BRUV methods. The assessment of the FT method aimed to identify the optimal soak time, and whether or not the size of the funnel entrance to the trap affected the catch. The results identified that larger funnel entrances caught more fish and soak times of 80 minutes produced the highest catches per unit effort. However the data were highly variable and the method detected few of the species typical of the region. Fish traps were also associated with high levels of mortality of fish post-release. The assessment of UVC strip transect method involved directly comparing the precision of data collected by researchers and volunteers using a novel double-observer technique (paired-transects). The results showed considerable error in both the volunteers and researchers data, however the researchers produced significantly higher precision data, compared to the volunteers. The distinction between researchers and volunteers was not evident in the data for the dominant species of fish. For all observers, the abundance of a species in the sample had a significant influence on its detectability, with locally scarce or rare species poorly detected. UVC was able to sample the majority of species typical of reefs in the region, however it appeared plagued by observer and detectability biases. The assessments of RUV and BRUV were conducted simultaneously which enabled the assessment of the effect of bait on the observed fish community. In addition the optimal deployment time for both methods to maximise species richness and abundance was determined. The results showed that BRUV, and to a lesser degree RUV, were able to effectively survey the reef fish community for the region with a 50 minute and 35 minute deployment time, respectively. Baited remote underwater video was especially good at detecting the invertebrate and generalist carnivores, and cartilaginous species. On the other hand, RUV was more effective at surveying the microinvertebrate carnivores. Remote underwater video was characterised by higher data variability, compared to BRUV, and was ultimately considered a less cost-efficient monitoring method. Comparative methods assessments were conducted during two field experiments with the FT, UVC and BRUV methods in the TMNP MPA, and the FT, CA, UVC, RUV, BRUV and ROV methods compared in the TNP MPA. The objectives of the comparison were to investigate differences in the fish communities observed with the different methods, and to determine the power of the data to detect an annual 10% growth in the fish populations over a period of five years. The results from the method comparison were in turn used to conduct the cost-benefit analysis to determine the efficiency of the different methods at achieving monitoring objectives requiring population data from multiple trophic and functional groups with the community, and from species of fisheries importance. The results indicated that FT, CA and ROV were ineffective at monitoring the reef fish community, although CA appeared to provide valuable data for the dominant fisheries species. Both CA and FT required minimal initial investment however, the variability in the data translated into high annual monitoring costs, as the required sampling effort was great. The ROV required the highest initial investment and was identified as the least cost-efficient method. Underwater visual census was able to adequately survey the bony fish within the community, however it did not detect the cartilaginous species. Underwater visual census required a large initial investment and was not cost-efficient, as a many samples were required to account for the variability in the data. Remote underwater video provided a comprehensive assessment of the reef fish community, however it too was associated with high levels of variability in the data, compared to BRUV, reducing its cost-efficiency. BRUV provided the most comprehensive assessment of the reef fish community and was associated with the highest cost-efficiency to address the community and fisheries species monitoring objectives. During the course of this research stereo-BRUV has gained considerable support as an effective reef fish monitoring method. Although not tested during this research, stereo-BRUV is preferred to BRUV as it provides accurate data on the size of fish. However, the initial investment of stereo-BRUV is over three times that required for the BRUV. Although it is recommended that a baited video technique be used for LTM in the Agulhas Ecoregion, the choice between BRUV and stereo-BRUV will depend on the specific objectives of the programme and the available budget at the implementing agency

Euphausiid population structure and grazing in the Indian sector of the Antarctic Polar Frontal Zone, during austral autumn

The trophodynamics of the numerically dominant euphausiid species within a region of high mesoscale oceanographic variability in the southwest Indian sector of the Antarctic Polar Frontal Zone (PFZ) were investigated during the austral autumns April/May) of 2004 and 2005. During the 2004 survey, sub-surface (200 m) temperature profiles indicated that an intense frontal feature, formed by the convergence of the Sub-Antarctic Front (SAF) and the Antarctic Polar Front (APF) bisected the survey area into two distinct zones, the Sub- Antarctic Zone (SAZ) and the Antarctic Zone (AAZ). Total integrated chlorophyll-a (chl-a) biomass was typical for the region ( 0.05). The multivariate analysis identified two interacting mechanisms controlling the distribution patterns, abundance and biomass of the various euphausiid species, namely (1) diel changes in abundance and biomass, (2) and restricted distribution patterns associated with the different water masses. Ingestion rates were determined for five euphausiid species. E. triacantha was found to have the highest daily ingestion rate ranging from 1 226.1 to 6 029.1 ng pigm ind⁻¹d⁻¹, while the lowest daily ingestion rates were observed in the juvenile Thysanoessa species (6.4 to 943.0 ng pigm ind⁻¹ d⁻¹). The total grazing impact of the selected euphausiids ranged from < 0.1 to 20.1 μg pigm m⁻²d⁻¹, corresponding to < 0.15 % of the areal chl-a biomass. The daily ration estimates of autotrophic carbon for the euphausiids suggested that phytoplankton represented a minor component in their diets, with only the sub-adult E. vallentini consuming sufficient phytoplankton to meet their daily carbon requirements. A cyclonic cold-core eddy spawned from the region of the APF located in the southwest Indian sector of the PFZ was the dominant feature during the 2005 survey. The total areal chl-a biomass throughout the region was low, ranging between 5.6 and 11.4 mg chl-a m⁻², and was significantly higher within the core of the eddy compared to the surrounding waters (p < 0.05). RMT-8 and WP-2 total euphausiid abundance and biomass estimates were high, and ranged from 0.004 to 0.36 ind m⁻³ and 0.065 to 1.21 mg dwt m⁻³, and from 0.01 to 18.2 ind m⁻³ and 0.01 to 15.7 mg dwt m⁻³, respectively. A distinct spatial pattern in the euphausiid community was evident with the Antarctic species, Euphausia frigida, E. triacantha and E. superba predominating within the core of the eddy, while the PFZ waters were characterized by the sub-Antarctic species, E. longirostris, Stylocheiron maximum, Nematoscelis megalops and Thysanoessa gregaria. The eddy edge acted as a transition zone where species from both regions co-occurred. Within the survey area the combined ingestion rate of the six numerically dominant euphausiid species ranged between 0.02 and 5.31 μg pigm m⁻²d¹, which corresponded to a loss of between < 0.001 and 0.11 % of the available chl-a biomass. E. triacantha and juvenile T. macura were identified as the dominant grazers. There was no apparent spatial pattern in the grazing activity of the euphausiids within the region of investigation. The average daily rations of the euphausiids examined were < 2 % of their body carbon. The low daily ration of the euphausiids could be ascribed to the predominance of small picophytoplankton in the region of investigation, which are too small to be grazed efficiently by larger zooplankton. The marked spatial patterns in species composition and the elevated abundance and biomass of euphausiids, suggest that the mesoscale eddies contribute to the spatial and temporal heterogeneity of the planktonic community of the PFZ and may represent important foraging regions for many of the apex predators within the region

New possibilities for research on reef fish across the continental shelf of South Africa

[From introduction] Subtidal research presents numerous challenges that restrict the ability to answer fundamental ecological questions related to reef systems. These challenges are closely associated with traditional monitoring methods and include depth restrictions (e.g. safe diving depths for underwater visual census), habitat destruction (e.g. trawling), mortality of target species (e.g. controlled angling and fish traps), and high operating costs (e.g. remotely operated vehicles and large research vessels. Whereas many of these challenges do not apply or are avoidable in the shallow subtidal environment, the difficulties grow as one attempts to sample deeper benthic habitats. This situation has resulted in a paucity of knowledge on the structure and ecology of deep water reef habitats around the coast of South Africa and in most marine areas around the world. Furthermore, the inability to effectively survey deep water benthic environments has limited the capacity of researchers to investigate connectivity between shallow and deep water habitats in a standardised and comparable fashion

New possibilities for research on reef fish across the continental shelf of South Africa

[From introduction] Subtidal research presents numerous challenges that restrict the ability to answer fundamental ecological questions related to reef systems. These challenges are closely associated with traditional monitoring methods and include depth restrictions (e.g. safe diving depths for underwater visual census), habitat destruction (e.g. trawling), mortality of target species (e.g. controlled angling and fish traps), and high operating costs (e.g. remotely operated vehicles and large research vessels. Whereas many of these challenges do not apply or are avoidable in the shallow subtidal environment, the difficulties grow as one attempts to sample deeper benthic habitats. This situation has resulted in a paucity of knowledge on the structure and ecology of deep water reef habitats around the coast of South Africa and in most marine areas around the world. Furthermore, the inability to effectively survey deep water benthic environments has limited the capacity of researchers to investigate connectivity between shallow and deep water habitats in a standardised and comparable fashion

The <i>Bal des Ardents</i> (1393), Thomas of Woodstock (1397) and Richard II (1400): Three Medieval Conspiracy Rumours and the Scots’ Mine Play (1608).

Assassination vehicles in Elizabethan and Jacobean tragedies sometimes involve meta-theatrical court festival massacres: court performances embedded within full-length drama, resulting in violent death or trauma to characters in the play. During his career as a playwright (c. 1600–08), John Marston pioneered the masquerade-within as a popular sub-category of court festival massacre. Were such underhand festival appropriations wholly inspired by stage precedents? Or did they also occur in real life? Whether its deaths were accidental or resulted from a botched assassination plot, the 1393 Bal des Ardents was hugely culturally and politically influential. Its continuing cultural afterlives bear witness to the geographical, chronological and social shockwaves of a medieval event whose impact illuminates the persistent collective trauma generated by extreme modern assassinations. My researches identify the conspiracy rumours encouraged in the wake of the 1393 Paris disaster and two English conspiracies of 1397 and 1400 linked to court festivals, as key to a fresh approach to the meta-theatrical court festival massacre, and to interpretation of two plays traditionally discussed together, which refer to these English conspiracies, Shakespeare’s Richard II and the anonymous Thomas of Woodstock. My analysis supports a post-Elizabethan dating of Woodstock, and encourages the hypothesis that it could be the so-called Scots’ Mine Play of 1608, the lost Jacobean play thought by some to have ended Marston’s career as a playwright