Analysis of the cytotoxic effect of chemotherapeutic agents on ALDH1^high and ALDH1^low populations sorted from (A–C) SW-982 and (D–F) SW-1353 cells.

<p>Both subopulations were treated with 0–5.0 µM doxorubicin, 0–5.0 µM epirubicin, 1–100 µM cisplatin, and measured after 48 h. Mean value ±SD of all measurements was fitted according the Hill equation. Significant differences on the individual concentrations were incorporated in the curves.</p

Proliferation analysis of ALDH1^high and ALDH1^low sarcoma cells.

<p>The immunohistochemical analysis using anti-Ki-67 proliferation marker revealed a decreased proliferation level of (A) SW-1353 ALDH1^low cells and compared to (B) SW-1353 ALDH1^high cells. (C–E) Dynamic proliferation curves for ALDH1^high and ALDH1^low cells seeded at 10,000 cells per well measured with the xCELLigence system.</p

Clonogenic activity of ALDH1^high and ALDH1^low cells.

<p>(A) The quantitation of the clone formation efficiency from SW-684, SW-982, and SW-1353 cells. Data from five independent experiments represent average colony count/well after 14 days. (B) Representative colony forming units from all three cell lines.</p

Primer Sequences used for real-time RT-PCR.

<p>Primer Sequences used for real-time RT-PCR.</p

Overview of all results including the corresponding significances.

<p>Overview of all results including the corresponding significances.</p

Aldehyde dehydrogenase 1 (ALDH1) expression in sarcoma cell lines using the Aldefluor® assay.

<p>Fluorescence versus forward scatter was shown in a density blot from (A) DEAB control cells and (B) ALDH1-expressing cells (called ALDH1^high). (C) ALDH1 expression in % of gated cells. The highest proportion of ALDH1^high cells is represented by SW-684 cells (1.77±0.9%; n = 12), SW-982 cells (2.23±1.0%; n = 11), and SW-1353 cells (2.69±1.3%; n = 8). (D) After two weeks cultured the ALDH1^high population generated a significant higher account of ALDH1^high cells. (E) The enhanced ALDH activity was also demonstrated by western blot.</p

The relation between clinico-pathological parameters and pre-operative Hb levels of patients with soft tissue sarcoma (n = 367).

<p>MPNST, malignant peripheral nerve sheath tumor.</p><p>The relation between clinico-pathological parameters and pre-operative Hb levels of patients with soft tissue sarcoma (n = 367).</p

Univariate and multivariate Cox proportional analysis regarding cancer-specific survival.

<p>Univariate and multivariate Cox proportional analysis regarding cancer-specific survival.</p

Univariate and multivariate Cox proportional analysis regarding overall survival.

<p>Univariate and multivariate Cox proportional analysis regarding overall survival.</p

Kaplan-Meier curve for cancer-specific survival regarding high (≥13 g/dl in males and ≥12 g/dl in females) versus low (<13 g/dl in males and <12 g/dl in females) hemoglobin levels (<i>p</i><0.001).

<p>Kaplan-Meier curve for cancer-specific survival regarding high (≥13 g/dl in males and ≥12 g/dl in females) versus low (<13 g/dl in males and <12 g/dl in females) hemoglobin levels (<i>p</i><0.001).</p