1 research outputs found
Diversity and distribution of haptophytes reavealed by environmental sequencing - a review. Perspectives in Phycology. Supplementary material.
<p><b></b>
</p><p><b>7. Fig. S1. </b>Maximum-likelihood
tree<b> </b>(RAxML v. 8.026,
GTRCAT) based on 971 haptophyte 18S rRNA gene sequences from Table S1, 451 are from
cultured strains and 520 from environmental clone libraries. Bootstrap values
are marked at the nodes. The colours indicate taxonomic groups.</p>
<p><b>8. Table S1. </b>Haptophyte 18S rRNA gene sequence reference database
including 971 sequences, 451 from cultures and 520 from environmental clone
libraries. See File S1 for description of its construction. Chimeras (32) identified
by uchime in mothur and by manual blast are listed on sheet 2.</p>
<p><b>9. Table S2</b>.
Information on selected, commonly used PCR primers for environmental sequencing
and metabarcoding of haptophytes.</p>
<p><b>10. File S1.</b>
Description of the construction of the Haptophyte 18S rRNA gene reference sequence
database, alignment and final RAxML phylogenetic tree.</p>
<p><b>11. File S2. </b>Fasta
file with accession numbers and 18S rRNA gene sequence of 971 reference
sequences for taxonomic assignment in mothur or Qiime. </p>
<p><b>12. File S3. </b>Taxonomy
file with systematic placement of 971 haptophyte reference sequences for
taxonomic assignment in mothur or Qiime. </p>
<p><b>13. File S4. </b>Curated
haptophyte 18S rRNA gene alignment for Figs 2 and S1.</p