1 research outputs found
Glycolate oxidase modulates photosynthetic activity and reactive oxygen species metabolism in Arabidopsis thaliana under salt stress
1 pĂĄgina - Poster presentado en Iberian Plant Biology 2023. XVIII Portuguese-Spanish Congress on Plant Biology and the XXV Meeting of the Spanish Society of Plant Biology. 9-12 Julio 2023, Braga, Portugal.Salt stress is one of the biggest environmental factors impacting plant growth and productivity in many
parts of the world, particularly in irrigated lands of arid and semiâarid regions. The involvement of
glycolate oxidase (GOX), an enzyme that catalyzes the conversion of glycolate into glyoxylate during
photorespiration with concomitant production of HâOâ, in the regulation of photosynthesis and reactive
oxygen species (ROS) metabolism was investigated in saltâchallenged Arabidopsis wild type and two gox
Arabidopsis mutants (gox1 and gox2). Plants were grown under hydroponic conditions supplemented or
not with 100 mM NaCl for 24 h. Under control conditions, pigments contents (chla, chlb, total
chlorophyll) were higher in WT than gox mutants. However, a different behavior was observed following
NaCl treatment. The same trend of pigment content was registered for gas exchange parameters. Under
control conditions, as expected, leaf GOX activity was reduced in gox mutants compared to WT, while
NaCl increased GOX activity only in gox mutants. Leaf HâOâ content was reduced in gox mutants
compared to WT under control and stress conditions. NaClâinduced oxidative stress, noticed by oncrease
of malondialdehyde content, was observed only in WT and gox1 mutant. Modulation of catalase,
guaiacol peroxidase, and glutathione reductase activitives in leaves were also registered in all genotypes
and depending on the treatment. As a whole, data show the crucial role of GOX in regulating both
photosynthetic activity and ROS metabolism under salinity conditions.This work was supported by IâCOOP grant (COOPA20471) from the CSIC, Spain and PID2021â122280NBâI00 and RED2018â102407âT from the Ministry of Science, Innovation and Universities and the European Regional Development Fund coâfunding (MCIU/AEI/ERDF