2 research outputs found
Patterned Fluoropolymer Barriers for Containment of Organic Solvents within Paper-Based Microfluidic Devices
In
this study, we demonstrate for the first time the ability to pattern
lipophobic fluoropolymer barriers for the incorporation of pure organic
solvents as operating liquids within paper-based microfluidic devices.
Our fabrication method involves replacing traditional wax barriers
with fluoropolymer coatings by combining initiated chemical vapor
deposition with inhibiting transition metal salt to pattern the polymer.
Multiple techniques for patterning the transition metal salt are tested
including painting, spray coating, and selective wetting through the
use of a photoresist. The efficacy of the barrier coatings to contain
organic solvents is found to be dependent on the conformality of the
polymer deposited around the paper fibers. We demonstrate examples
of the benefits provided by the containment of organic solvents in
paper-based microfluidic applications including the ability to tune
the separation of analytes by varying the operating solvent and by
modifying the channel region of the devices with additional polymer
coatings. The work exhibited in this paper has the potential to significantly
expand the applications of paper-based microfluidics to include detection
of water insoluble analytes. Additionally, the generality of the patterning
process allows this technique to be extended to other applications
that may require the use of patterned hydrophobic and lipophobic regions,
such as biosensing, chemical detection, and optics
Additional file 1: Figure S1. of Recovery of a human natural antibody against the noncollagenous-1 domain of type IV collagen using humanized models
Sequences of rearranged Ig heavy chain variable region genes of human anti-alpha3(IV)NC1 collagen monoclonal antibody 2D6. Sequence of mAb 2D6 heavy chain V-D-J region and sequences of the closest corresponding germline variable region gene segments identified in the IMGT/V-QUEST reference databases, with CDR-IMGT delineated according to the IMGT unique numbering for V-DOMAIN