Identification of translocation chromosomes.

<p>(A)–(D), FISH and GISH using rye genomic DNA (red), pSc119.2(green), and pAs1 (red) as probes. (A) and (B), Wheat and rye parents. (C) and (D), new primary 1RS.1BL translocation lines. (E), FISH using 6c6 (green) and telomere sequence (red) as probes, indicating the integrity of all chromosomes. (F), FISH using 6c6 (green) and pAWRC.1 (red) as probes, indicating that the 1RS arm is intact in the translocation.</p

A-PAGE separations of ω-secalins and gliadins from a new primary 1RS.1BL translocation line and its wheat and rye parents.

<p>(L to R) 1 = wheat parent A42912, 2 = Weining rye, 3 = Chuan-nong 10, 4 = Chuan-nong 17, 5–8 = different lines of T917-26, the seeds from two years, 9 and 10 = different lines of T917-15. Arrowheads indicate the absence of expression of ω-secalins.</p

The sequences of SSR markers which were specific for 1RS chromosome.

<p>The sequences of SSR markers which were specific for 1RS chromosome.</p

PCR result of primer PrCEN-2.

<p>Lane 1 = RT1249; lane 2 = RT1217-1; lane 3 = RT1163-4; lane 4 = RS1200-3; lane 5 = Aigan rye; lane 6 = MY11; lane M = marker.</p

PCR results of 4 specific primers: O11B3 and O11B5, ω-sec-P1 and ω-sec-P2.

<p>Lane 1 = RT1249; lane 2 = RT1163-4; lane 3 = RT1217-11; lane 4 = RS1200-3; lane 5 = CN11; lane 6 = MY11; lane 7 = Aigan rye; lane M = marker.</p

Comparisons of agronomic traits among substitution, translocation lines and their wheat parent.

<p>Comparisons of agronomic traits among substitution, translocation lines and their wheat parent.</p

A-PAGE separations of ω-secalins and gliadins from primary 1RS.1BL translocation lines and the substitution line.

<p>Lane 1 = wheat parent MY11; lane 2 = RT1249; lane 3 = RT1217-1; lane 4 = RT1163-4; lane 5 = RS1200-3; lane 6 = CN11.</p

FISH of root tip chromosomes in wheat-rye translocation and substitution lines which originated from MY11 x Aigan rye.

<p>The arrows indicate wheat-rye translocated chromosomes or 1R substituted chromosomes. A. RT1249; B. RT1163-4; C. RT1217-1; D. RS1200-3.</p

PCR results of primer TNAC1021.

<p>Lane 1 = MY11; lane 2 = water; lane 3 = RS1200-3; lane 4 = RT1249; lane 5 = RT1217-1; lane 6 = RT1163-4; lane 7 = CN11; lane M = marker.</p

Resistant analysis of translocation/substitution lines and their wheat parent to stripe rust when inoculated with epidemic pathotypes and isolates of <i>Puccinia striiformis</i> f. sp. <i>Tritici</i>, <i>(Pst)</i>.

<p>Resistant analysis of translocation/substitution lines and their wheat parent to stripe rust when inoculated with epidemic pathotypes and isolates of <i>Puccinia striiformis</i> f. sp. <i>Tritici</i>, <i>(Pst)</i>.</p