Sugar additives for MALDI matrices improve signal allowing the smallest nucleotide change (A:T) in a DNA sequence to be resolved

Sample preparation for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) of DNA is critical for obtaining high quality mass spectra. Sample impurity, solvent content, substrate surface and environmental conditions (temperature and humidity) all affect the rate of matrix–analyte co-crystallization. As a result, laser fluence threshold for desorption/ionization varies from spot to spot. When using 3-hydroxypicolinic acid (3-HPA) as the matrix, laser fluence higher than the threshold value reduces mass resolution in time-of-flight (TOF) MS as the excess energy transferred to DNA causes metastable decay. This can be overcome by either searching for ‘hot’ spots or adjusting the laser fluence. However, both solutions may require a significant amount of operator manipulation and are not ideal for automatic measurements. We have added various sugars for crystallization with the matrix to minimize the transfer of excess laser energy to DNA molecules. Fructose and fucose were found to be the most effective matrix additives. Using these additives, mass resolution for DNA molecules does not show noticeable deterioration as laser energy increases. Improved sample preparation is important for the detection of single nucleotide polymorphisms (SNPs) using primer extension with a single nucleotide. During automatic data acquisition it is difficult to routinely detect heterozygous A/T mutations, which requires resolving a mass difference of 9 Da, unless a sugar is added during crystallization

Shock compression of liquid hydrazine

Liquid hydrazine (N{sub 2}H{sub 4}) is a propellant used by the Air Force and NASA for aerospace propulsion and power systems. Because the propellant modules that contain the hydrazine can be subject to debris impacts during their use, the shock states that can occur in the hydrazine need to be characterized to safely predict its response. Several shock compression experiments have been conducted in an attempt to investigate the detonability of liquid hydrazine; however, the experiments results disagree. Therefore, in this study, we reproduced each experiment numerically to evaluate in detail the shock wave profiles generated in the liquid hydrazine. This paper presents the results of each numerical simulation and compares the results to those obtained in experiment. We also present the methodology of our approach, which includes chemical kinetic experiments, chemical equilibrium calculations, and characterization of the equation of state of liquid hydrazine

Chandra and Hubble Study of a New Transient X-ray Source in M31

We present X-ray and optical observations of a new transient X-ray source in M31 first detected 23-May-2004 at R.A.=00:43:09.940 +/- 0.65'', Dec.=41:23:32.49 +/- 0.66''. The X-ray lightcurve shows two peaks separated by several months, reminiscent of many Galactic X-ray novae. The location and X-ray spectrum of the source suggest it is a low mass X-ray binary (LMXB). Follow-up HST ACS observations of the location both during and after the outburst provide a high-confidence detection of variability for one star within the X-ray position error ellipse. This star has $\Delta$B ~ 1 mag, and there is only a ~1% chance of finding such a variable in the error ellipse. We consider this star a good candidate for the optical counterpart of the X-ray source. The luminosity of this candidate provides a prediction for the orbital period of the system of 2.3$^{+3.7}_{-1.2}$ days.Comment: 17 pages, 3 figures, 4 tables, accepted for publication in Ap

Computational Fluid Dynamics (CFD) Analyses in Support of Space Shuttle Main Engine (SSME) Heat Exchanger (HX) Vane Cracking Investigation

Integration issues involved with installing the alternate turbopump (ATP) High Pressure Oxygen Turbopump (HPOTP) into the SSME have raised questions regarding the flow in the HPOTP turnaround duct (TAD). Steady-state Navier-Stokes CFD analyses have been performed by NASA and Pratt & Whitney (P&W) to address these questions. The analyses have consisted of two-dimensional axisymmetric calculations done at Marshall Space Flight Center and three-dimensional calculations performed at P&W. These analyses have identified flowfield differences between the baseline ATP and the Rocketdyne configurations. The results show that the baseline ATP configuration represents a more severe environment to the inner HX guide vane. This vane has limited life when tested in conjunction with the ATP but infinite life when tested with the current SSME HPOTP. The CFD results have helped interpret test results and have been used to assess proposed redesigns. This paper includes details of the axisymmetric model, its results, and its contribution towards resolving the problem

Dehydration accelerates reductions in cerebral blood flow during prolonged exercise in the heat without compromising brain metabolism

Dehydration hastens the decline in cerebral blood flow (CBF) during incremental exercise, while the cerebral metabolic rate for oxygen (CMRO2) is preserved. It remains unknown whether CMRO2 is also maintained during prolonged exercise in the heat and whether an eventual decline in CBF is coupled to fatigue. Two studies were undertaken. In study 1, ten male cyclists cycled in the heat for ~2 h with (control) and without fluid replacement (dehydration) while internal (ICA) and external (ECA) carotid artery blood flow and core and blood temperature were obtained. Arterial and internal jugular venous blood samples were assessed with dehydration to evaluate the CMRO2. In study 2 (8 males), middle cerebral artery blood velocity (MCA Vmean) was measured during prolonged exercise to exhaustion in both dehydrated and euhydrated states. After a rise at the onset of exercise, ICA flow declined to baseline with progressive dehydration (P < 0.05). However, cerebral metabolism remained stable through enhanced oxygen and glucose extraction (P < 0.05). ECA flow increased for one hour but declined prior to exhaustion. Fluid ingestion maintained cerebral and extra-cranial perfusion throughout non-fatiguing exercise. During exhaustive exercise, however, euhydration delayed but did not prevent the decline in cerebral perfusion. In conclusion, during prolonged exercise in the heat dehydration accelerates the decline in CBF without affecting CMRO2 and also restricts extra-cranial perfusion. Thus fatigue is related to reduction in CBF and extra-cranial perfusion rather than in CMRO2.The study was supported by a grant from the Gatorade Sports Science Institute, PepsiCo Inc, USA

Optical Constraints on an X-ray Transient Source in M31

We have detected a transient X-ray source in the M31 bulge through a continuing monitoring campaign with the Chandra ACIS-I camera. The source was detected at R.A.=00:42:33.428 +/- 0.11'', Dec.=+41:17:03.37 +/- 0.11'' in only a single observation taken 2004 May 23. Fortuitous optical HST/ACS imaging of the transient location prior to the X-ray outburst, along with follow-up HST/ACS imaging during and after the outburst, reveals no transient optical source brighter than B (equivalent) = 25.5. The location of the source and its X-ray properties suggest it is a low mass X-ray binary (LMXB). Assuming the transient is similar to many Galactic X-ray novae, the X-ray luminosity of (3.9 +/- 0.5) X 10$^{37}$ erg s$^{-1}$ and the upper-limit on the optical luminosity provide a prediction of <1.6 days for the orbital period of the binary system.Comment: 15 pages, 3 figures, accepted for publication in Ap

A Catalog of Transient X-ray Sources in M31

From October 1999 to August 2002, 45 transient X-ray sources were detected in M31 by Chandra and XMM-Newton. We have performed spectral analysis of all XMM-Newton and Chandra ACIS detections of these sources, as well as flux measurements of Chandra HRC detections. The result is absorption-corrected X-ray lightcurves for these sources covering this 2.8 year period, along with spectral parameters for several epochs of the outbursts of most of the transient sources. We supply a catalog of the locations, outburst dates, peak observed luminosities, decay time estimates, and spectral properties of the transient sources, and we discuss similarities with Galactic X-ray novae. Duty cycle estimates are possible for 8 of the transients and range from 40% to 2%; upper limits to the duty cycles are estimated for an additional 15 transients and cover a similar range. We find 5 transients which have rapid decay times and may be ultra-compact X-ray binaries. Spectra of three of the transients suggest they may be faint Galactic foreground sources. If even one is a foreground source, this suggests a surface density of faint transient X-ray sources of >~1 deg$^{-2}$.Comment: 63 pages, 22 figures, 3 tables, accepted for publication in Ap

A Soft X-ray Transient in the M31 Bulge

We have examined a probable soft X-ray transient source in the M31 bulge at R.A.=0:42:41.814 +/- 0.08", Dec. = 41:16:35.86 +/- 0.07". On the three occasions we observed the source, its spectrum was soft (kT_{in} ~1 keV). The brightest detection of the source was 2004 July 17 with a 0.3-7 keV luminosity of ~5 X 10^{37} erg/s. The only previous detection of the source was in 1979 by the Einstein observatory. The multiple detections over 25 years suggest the duty cycle of the source is in the range 0.02-0.06. Coordinated HST/ACS imaging before, during, and after the outburst revealed no variable optical source within the position errors of the X-ray source. The optical data place a firm upper limit on the brightness of the counterpart of the X-ray outburst of B>24.7, suggesting the binary has a period <5.2 days. The X-ray spectrum and lack of bright stars at the source location indicate the source was a soft transient event occurring in a low-mass X-ray binary, making this source a good black hole candidate in M31.Comment: 18 pages, 4 tables, 3 figures, accepted for publication in Ap

X-ray/Optical/Radio Observations of a Resolved Supernova Remnant in NGC 6822

The supernova remnant (SNR), Ho 12, in the center of the dwarf irregular galaxy NGC 6822 was previously observed at X-ray, optical, and radio wavelengths. By using archival Chandra and ground-based optical data, we found that the SNR is spatially resolved in X-rays and optical. In addition, we obtained a ~5" resolution radio image of the SNR. These observations provide the highest spatial resolution imaging of an X-ray/optical/radio SNR in that galaxy to date. The multi-wavelength morphology, X-ray spectrum and variability, and narrow-band optical imagings are consistent with a SNR. The SNR is a shell-shaped object with a diameter of about 10" (24 pc). The morphology of the SNR is consistent across the wavelengths while the Chandra spectrum can be well fitted with a nonequilibrium ionization model with an electron temperature of 2.8 keV and a 0.3-7 keV luminosity of 1.6e37 erg/s. The age of the SNR is estimated to be 1700-5800 years.Comment: 6 pages, 3 figures, accepted for publication in the Astronomical Journa

Variable Star Candidates in an ACS Field of M31

A search for variable stars is performed using two epochs of Hubble Space Telescope (HST) Advanced Camera for Surveys (ACS) imaging data for a 9.28 square arcminute portion of M31. This data set reveals 254 sources that vary by at least 4-sigma between epochs. The positions and 2-epoch B-band (equivalent) photometry of these sources are presented. The photometry suggests that this catalog includes most of the RR Lyrae population of this portion of M31.Comment: 22 pages, 3 tables, 5 figures, accepted for publication in A