uPAR immunohistochemistry in urothelial neoplasia of the bladder.

<p>The figure shows examples of different uPAR scores at the invasive front of the tumour: uPAR score = 0 (no uPAR positive cells detected), uPAR score = 3 (between 5% and 10% positive cells), and uPAR score = 6 (>70% positively stained cells). Tissue sections stained with a pAb against uPAR. uPAR expression was scored semi-quantitatively. The antibody was visualised with NovaRed. uPAR immunoreactivity was scored separately in cancer cells, macrophages and myofibroblasts. The black squares in A, C and E are shown in higher magnification in B, D and F. uPAR immunoreactivity was primarily seen in myofibroblasts (<b>yellow</b> arrow in D and F) and macrophages (<b>green</b> arrow in D and F) in the surrounding stroma. uPAR positive neutrophils served as internal control (<b>red</b> arrow in B, D and F). Tu: tumour, Ca: cancer, St: stroma. Bar in A, C and E ~ 50μm. Bar in B, D and F ~ 25μm.</p

Distribution of uPAR positivity at the invasive front (A) and tumour core (B), respectively.

<p>uPAR positivity in both myofibroblasts and macrophages, but not cancer cells, increases significantly with tumour stage. The x-axis shows the tumour stage and the y-axis the percentage of uPAR positive cells. P-values shown are for the χ2-test.</p