Fenobam induces place preference in female SNI mice only.

<p>(<b>A</b>) SNI mice (n = 10) spent significantly more total time in the fenobam-paired chamber compared to vehicle-paired chamber on post-conditioning day 5. (<b>B</b>) Sham mice (n = 11) show no difference in the total amount of time spent in fenobam and vehicle-paired chamber on post-conditioning day 5. (<b>C</b>) SNI mice increase the amount of time they spend in the fenobam-paired chamber on day 5 (post-conditioning) compared to baseline (day 1 pre-conditioning) while (<b>D</b>) sham mice show no such difference. Paired t-test, *p<0.05, **p<0.01.</p

Morphine induces place preference in all male mice.

<p>(<b>A</b>) SNI mice (n = 9) spend significantly more total time in the morphine-paired chamber compared to vehicle-paired chamber on post-conditioning day 5. (<b>B</b>) Sham mice (n = 15) showed a similar significant preference. (<b>C</b>) SNI mice increase the amount of time they spent in the morphine-paired chamber on day 5 (post-conditioning) compared to baseline (day 1 pre-conditioning) while (<b>D</b>) sham mice showed a similar significant preference. Paired t-test, *p<0.05, **p<0.01.</p

Male SNI and sham mice travel similar distances and show tolerance to locomotor side-effects.

<p>(<b>A</b>) SNI (n = 15) and sham (n = 15) mice travel a similar distance in both outer chambers of the aCPP apparatus on preconditioning day 1. (<b>B</b>) On conditioning day 2 and (<b>C</b>) conditioning day 3, both SNI and sham mice are hyperactive when treated with fenobam. Mice were restricted to only one of the outer chambers when these measurements were taken. (<b>D</b>) However, by day 4, neither group of mice show increased distance traveled in the fenobam chamber compared to the vehicle-paired chamber. Two-way ANOVA followed by Bonferroni <i>post hoc</i> test, **p<0.01, ***p<0.001.</p

MPEP induces place preference in male SNI mice only.

<p>(<b>A</b>) SNI mice (n = 8) spent significantly more total time in the MPEP-paired chamber compared to vehicle-paired chamber on post-conditioning day 5. (<b>B</b>) Sham mice (n = 8) show no difference in the total amount of time spent in MPEP and vehicle-paired chamber on post-conditioning day 5. (<b>C</b>) SNI mice increase the amount of time they spend in the MPEP-paired chamber on day 5 (post-conditioning) compared to baseline (day 1 pre-conditioning) while (<b>D</b>) sham mice show no such difference. Paired t-test, *p<0.05, **p<0.01.</p

Animal's body temperature affects VMR.

<p>Following long anesthesia, animals (n = 6) were placed through three sets of graded distensions at 37.5, 33.5, and 35.5°C. Two-way ANOVA yielded a significant main effect of temperature (***p<0.0001), but not of pressure (p = 0.4536). Bonferroni's posttest revealed differences between 37.5 and 33.5°C at 60 mmHg and 75 mmHg (***p<0.001), as well as differences between 37.5 and 35.5°C at 60 mmHg (***p<0.001) and 75 mmHg (*p<0.05), and differences between 35.5 and 33.5°C at 75 mmHg (*p<0.05).</p

Visceromotor responses (VMR) from urinary bladder distension (UBD).

<p>(A) Schematic of UBD setup. Compressed air is delivered into bladder via urethral catheter. During distensions, electrodes in abdominal muscle record EMG. Temperature is maintained throughout the procedure using a battery operated heating pad and overhead lamp. (B) Example EMG traces during UBD. As pressure increases, electrical output from abdominal muscles increases congruently. (C) Example VMR from one complete set of distensions. During each set, the bladder is distended three times at each pressure. VMRs are normalized with 20 s pre-distension interval then averaged for each pressure.</p

Fenobam induces place preference in male SNI mice only.

<p>(<b>A</b>) SNI mice (n = 15) spent significantly more total time in the fenobam-paired chamber compared to vehicle-paired chamber on post-conditioning day 5. (<b>B</b>) Sham mice (n = 15) show no difference in the total amount of time spent in fenobam and vehicle-paired chamber on post-conditioning day 5. (<b>C</b>) SNI mice increase the amount of time they spent in the fenobam-paired chamber on day 5 (post-conditioning) compared to baseline (day 1 pre-conditioning) while (<b>D</b>) sham mice show no such difference. Paired t-test, *p<0.05, **p<0.01.</p

Morphine induces place preference in all female mice.

<p>(<b>A</b>) SNI mice (n = 5) spend significantly more total time in the morphine-paired chamber compared to vehicle-paired chamber on post-conditioning day 5. (<b>B</b>) Sham mice (n = 6) show a similar significant preference. (<b>C</b>) SNI mice increase the amount of time they spent in the morphine-paired chamber on day 5 (post-conditioning) compared to baseline (day 1 pre-conditioning). (<b>D</b>) Sham mice show a similar significant preference. Paired t-test, *p<0.05, **p<0.01, ***p<0.001.</p