Training Models for Enucleation and Orbital Compartment Syndrome

ME450 Capstone Design and Manufacturing Experience: Fall 2021Enucleation and lateral canthotomy are two ophthalmic surgeries with little or no training models. Residents learn these procedures in the operating room by watching faculty and then advancing to take over more steps. The goal of this project is to build training models that will give the residents realistic training on the most important steps of both procedures while also being reusable, high-fidelity, and inexpensive. The models will provide training to residents and doctors in low-resource countries as well as the US to reduce post-operative complications and improve surgical quality.Dr. Christine Nelson, MD, FACS: Kellogg Eye Centerhttp://deepblue.lib.umich.edu/bitstream/2027.42/172490/1/Team22-TrainingModelsForEnucleationAndLateralCanthotomy.pd

Sex-dependent treatment of chronic EAE with partial MHC class II constructs

Abstract Background One of the main challenges in treating multiple sclerosis (MS) is reversing the effects of accumulated damage in the central nervous system (CNS) of progressive MS subjects. While most of the available drugs for MS subjects are anti-inflammatory and thus are limited to relapsing-remitting MS subjects, it is not clear to what extent their effects are capable of inducing axonal repair and remyelination in subjects with chronic MS. Methods A chronic model of experimental autoimmune encephalomyelitis (EAE) was used to evaluate the potency of partial MHC (pMHC) class II constructs in treating progressive EAE. Results We demonstrated an estrogen receptor alpha (ERα)-dependent increased dose requirement for effective treatment of female vs. male mice using pMHC. Such treatment using 100-μg doses of RTL342M or DRα1-mMOG-35-55 constructs significantly reversed clinical severity and showed a clear trend for inhibiting ongoing CNS damage, demyelination, and infiltration of inflammatory cells into the CNS in male mice. In contrast, WT female mice required larger 1-mg doses for effective treatment, although lower 100-μg doses were effective in ovariectomized or ERα-deficient mice with EAE. Conclusions These findings will assist in the design of future clinical trials using pMHC for treatment of progressive MS

Additional file 1: Figure S1. of Sex-dependent treatment of chronic EAE with partial MHC class II constructs

EAE disease course in female and male (A) DR*1501-Tg and (B) C57BL/6 mice, immunized with mMOG-35-55 peptide. Figure S2. Days 20 and 63 p.i. spinal cord lumbar sections from EAE female DR*1501-Tg mice were stained with luxol fast blue (LFB) and analyzed for demyelination, toluidine blue for spinal cord damage, and CD4+ cell frequency in the spinal cords of RTL342M- vs. vehicle-treated mice. (PDF 231 kb