Aktivitas Antioksidan Ekstrak, Fraksi Dan Golongan Senyawa Kimia Daun Premna Oblongata Miq.

Indonesia has many kinds of plants which can be used as antioxidant sources. This study is focused on antioxidant activity of Cincau Perdu leaves extract (Premna oblongata Miq.) by1,1-Diphenyl-2-picrylhydrazyl (DPPH) methods. Premna oblongata Miq.leaves was extracted using n-hexane, ethyl acetate and methanol. The IC50 value of methanol extract as the highest activity as antioxidantwas 20.01 μg/mL. The extract which had the highest antioxidant activity were fractionated by accelerated column chromatography and were earned 6 combination fractions. The antioxidant activity of each combination fractions were tested by DPPH methods. Fraction-5 showed the highest activity as antioxidant with IC50 23.51 μg/mL. The compounds of the active fractions were flavonoid, glikon, saponin and tanin