A 'Divorce Blueprint'? The Use of Heteronormative Strategies in Addressing Economic Inequalities on Civil Partnership Dissolution

This article will explore data obtained through interviews with UK family law practitioners and clients with experience of financial relief on formalised same-sex relationship breakdown. It will focus on questions around how solicitors have approached and argued their dissolution cases (and the extent to which they have drawn upon heteronormative arguments and case law), and whether both they and the clients believed that civil partnerships are, and should be, treated similarly to marriages. Th e discussion will examine the different understandings of ?equality? employed, and question the ways that the participants relied on ideas of sameness and difference. It will be argued that the solicitors placed particular stress on sameness, and that heteronormative constructs of gendered inequalities have been transplanted into same-sex cases, in a system where practitioners? submissions are based on ?what works.? Th is is despite the fact that lesbian and gay couples do not map onto the ?template? under which the parties have been subjected to different gendered expectations. Conversely, the clients were less willing to take on the full legal implications associated with (heterosexual) marital breakdown and less receptive of the solicitors ?translating? their matters to pigeonhole them into the existing framework

Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cells.

Cellular products derived from the activity of DNA, RNA, and protein synthesis collectively control cell identity and function. Yet there is little information on how these three biosynthesis activities are coordinated during transient and sparse cellular processes, such as activation and differentiation. Here, we describe Simultaneous Overview of tri-Molecule Biosynthesis (SOM3B), a molecular labeling and simultaneous detection strategy to quantify DNA, RNA, and protein synthesis in individual cells. Comprehensive interrogation of biosynthesis activities during transient cell states, such as progression through cell cycle or cellular differentiation, is achieved by partnering SOM3B with parallel quantification of select biomolecules with conjugated antibody reagents. Here, we investigate differential de novo DNA, RNA, and protein synthesis dynamics in transformed human cell lines, primary activated human immune cells, and across the healthy human hematopoietic continuum, all at a single-cell resolution

A Universal Live Cell Barcoding-Platform for Multiplexed Human Single Cell Analysis.

Single-cell barcoding enables the combined processing and acquisition of multiple individual samples as one. This maximizes assay efficiency and eliminates technical variability in both sample preparation and analysis. Remaining challenges are the barcoding of live, unprocessed cells to increase downstream assay performance combined with the flexibility of the approach towards a broad range of cell types. To that end, we developed a novel antibody-based platform that allows the robust barcoding of live human cells for mass cytometry (CyTOF). By targeting both the MHC class I complex (beta-2-microglobulin) and a broadly expressed sodium-potassium ATPase-subunit (CD298) with platinum-conjugated antibodies, human immune cells, stem cells as well as tumor cells could be multiplexed in the same single-cell assay. In addition, we present a novel palladium-based covalent viability reagent compatible with this barcoding strategy. Altogether, this platform enables mass cytometry-based, live-cell barcoding across a multitude of human sample types and provides a scheme for multiplexed barcoding of human single-cell assays in general

Heteronormativity in dissolution proceedings: Exploring the impact of recourse to legal advice in same sex relationship breakdown

This chapter explores how heteronormativity, normative ordering of society to correspond with heterosexuality, shapes experiences of dissolution of formally recognized same-sex relationships. We present qualitative data from in-depth interviews with both clients and solicitors with direct experience of civil partnership dissolution. Drawing on insights from legal-consciousness studies, we explore the extent to which legal intervention in relationship breakdown creates an arena of strategy and self-interest. Overall, these data demonstrate the ways in which ‘law’ is conceived of as a product of its actors, rather than as being an entity of ‘the state’. We show that heteronormative understandings of gender roles in relationships have been carried over from (different-sex) marriage into civil-partnership proceedings. We argue that lesbians and gay men retain a level of resistance to this legal heteronormativity that has the potential to have transformative effects on contemporary understandings of the place of gender in marriage.</p

Emotional real-world scenes impact visual search

Research shows that emotional stimuli can capture attention, and this can benefit or impair performance, depending on the characteristics of a task. Additionally, whilst some findings show that attention expands under positive conditions, others show that emotion has no influence on the broadening of attention. The current study investigated whether emotional real-world scenes influence attention in a visual search task. Participants were asked to identify a target letter embedded in the centre or periphery of emotional images. Identification accuracy was lower in positive images compared to neutral images, and response times were slower in negative images. This suggests that real-world emotional stimuli have a distracting effect on visual attention and search. There was no evidence that emotional images influenced the spatial spread of attention. Instead, it is suggested that findings may provide support for the argument that positive emotion encourages a global processing style and negative emotion promotes local processing

Proliferation tracing with single-cell mass cytometry optimizes generation of stem cell memory-like T cells.

Selective differentiation of naive T cells into multipotent T cells is of great interest clinically for the generation of cell-based cancer immunotherapies. Cellular differentiation depends crucially on division state and time. Here we adapt a dye dilution assay for tracking cell proliferative history through mass cytometry and uncouple division, time and regulatory protein expression in single naive human T cells during their activation and expansion in a complex ex vivo milieu. Using 23 markers, we defined groups of proteins controlled predominantly by division state or time and found that undivided cells account for the majority of phenotypic diversity. We next built a map of cell state changes during naive T-cell expansion. By examining cell signaling on this map, we rationally selected ibrutinib, a BTK and ITK inhibitor, and administered it before T cell activation to direct differentiation toward a T stem cell memory (TSCM)-like phenotype. This method for tracing cell fate across division states and time can be broadly applied for directing cellular differentiation

Mapping lung cancer epithelial-mesenchymal transition states and trajectories with single-cell resolution.

Elucidating the spectrum of epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) states in clinical samples promises insights on cancer progression and drug resistance. Using mass cytometry time-course analysis, we resolve lung cancer EMT states through TGFβ-treatment and identify, through TGFβ-withdrawal, a distinct MET state. We demonstrate significant differences between EMT and MET trajectories using a computational tool (TRACER) for reconstructing trajectories between cell states. In addition, we construct a lung cancer reference map of EMT and MET states referred to as the EMT-MET PHENOtypic STAte MaP (PHENOSTAMP). Using a neural net algorithm, we project clinical samples onto the EMT-MET PHENOSTAMP to characterize their phenotypic profile with single-cell resolution in terms of our in vitro EMT-MET analysis. In summary, we provide a framework to phenotypically characterize clinical samples in the context of in vitro EMT-MET findings which could help assess clinical relevance of EMT in cancer in future studies