EVALUATION OF IN VITRO ANTIOXIDANT POTENTIAL OF SPENT CHILLI AND SPENT CUMIN OBTAINED AFTER OLEORESIN EXTRACTION

Objective: Chilli spent residue (CHSR) and Cumin spent residue (CSR) obtained after oleoresin extraction is known to be rich in polyphenols, however, the very limited commercial application is known except for its use as veterinary feeds. Considering that a huge residue is left over by oleoresin spice industry, application-oriented utilization of spent residue of chilli and cumin is warranted. In these lines, we in this study evaluate the antioxidant potential of Chilli spent residue (CHSR) and Cumin spent residue (CSR) obtained after oleoresin extraction by DPPH method.Methods: In vitro radical scavenging activity of CHSR and CSR obtained after oleoresin extraction was evaluated by checking its role in scavenging DPPH.Results: The spent chilli extract exhibited higher DPPH scavenging activity when compared to the spent cumin and the IC50 values of spent chilli; spent cumin and ascorbic acid were found to be 186.23±1.05 µg/ml, 284±1.03 µg/ml and 33.21±1.04, respectively. As CHSR and CSR obtained after oleoresin extraction is known to be rich in polyphenols, these might be responsible for potent and significant antioxidant activity observed.Conclusion: This study shows that by-products obtained/generated in oleoresin industry can be utilized as value added product. Future work will be interesting to know the chemical composition and better understand the mechanism of action of the antioxidants present in the extract for development as a drug for therapeutic application.Â

Synthesis of Novel 3-Aryl-N-Methyl-1,2,5,6-Tetrahydropyridine Derivatives by Suzuki coupling: As Acetyl Cholinesterase Inhibitors

Alzheimer’s disease (AD) is a neurodegenerative disorder affecting the central nervous system, which is also associated with progressive loss of memory and cognition. The development of numerous structural classes of compounds with different pharmacological profile could be an evolving, promising therapeutic approach for the treatment of AD. Thus, providing a symptomatic treatment for this disease are cholinomimetics with the pharmacological profile of Acetylcholinesterase (AChE) inhibitors. In view of this, we have synthesized novel 3-aryl-N-methyl-1,2,5,6-tetrahydropyridine derivatives 5a-k by Suzuki coupling and screened the efficacy of these derivatives for their AChE inhibitor activity

THE ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF THE LEAVES EXTRACT OF CLERODENDRUM COLEBROOKIANUM WALP, (FAM: VERBENACEAE)

Objective: To investigate the in-vitro antioxidant and antimicrobial potential of Clerodendrum colebrookianum leaves extract.Methods: The leaves of C. colebrookianum were collected from various parts of Aizawl, Mizoram, India. Subsequently, the leaves were extracted with solvents (chloroform, acetone, ethanol and methanol) in a Soxhlet extraction apparatus for 24hr. Further, the extracts were extensively examined for its in-vitro antioxidant (DPPH) and antimicrobial activities. The preliminary phytochemical screening was carried out using standard protocols.Results: The existence of alkaloids, flavonoids, diterpenes, saponins, glycosides, steroids and terpeinoids were revealed in the phytochemical screening. The aqueous and acetone extract had the highest total phenolic content (2.348 mg/ml), when compared to methanol, ethanol and chloroform extracts, which was 0.549 mg/ml, 0.408 mg/ml and 0.407 mg/ml, respectively. The antioxidant activity was more significant for aqueous extract, when compared to other extracts. The antimicrobial activity was more significant for acetone extract showed significant zone of inhibition of 14±0.3, 13±0.3 and 15±0.2 for E. coli, S. marcescens and S. aureus, respectively.Conclusion: The high level of antioxidant and antimicrobial potential of C. colebrookianum leaf extracts encourage its potential use for biomedical applications

Synthesis of 3-Trifluoromethyl-5,6-dihydro-[1,2,4]triazolo Pyrazine Derivatives and Their Anti-Cancer Studies

The synthesis of a wide variety of 3-trifluoromethyl-5,6-dihydro-[1,2,4]triazolo pyrazine derivatives, by the treatment of 3-trifluoromethyl-5,6,7,8-tetrahydro-[1,2,4]triazolo[4,3-α]pyrazine hydrochloride with an array of isocyanates in the presence of triethylamine, is reported. All the target compounds were synthesized in excellent yields under mild reaction conditions. The target molecules were effectively screened for their anti-cancer properties and the results are promising. The resultant compounds were assessed for their antiproliferative action against two human colon cancer cell lines (HCT-116 and HT-29 colon cancer cell lines). The IC50 range was estimated at 6.587 to 11.10 µM showing that compound RB7 had remarkable anticancer movement on HT-29. Additionally, it was discovered that RB7 incited the mitochondrial apoptotic pathway by up-regulating Bax and down-regulating Bcl2, eventually leading to the activation of Caspase 3 in HT-29 cells and initiation of cell death via the mitochondrial apoptotic pathway

[1-(4-Chloro-2-fluoro­phenyl­sulfonyl)­piperidin-4-yl]di­phenyl­methanol

In the title compound, C24H23ClFNO3S, the piperidine ring is in a chair conformation. The geometry around the S atom is distorted tetra­hedral. The dihedral angle between the least-squares plane, P1, defined by four C atoms of the piperidine ring, and the dihalo-substituted benzene ring is 49.80 (1)°. The dihedral angles between P1 and the two phenyl rings are 59.34 (1) and 73.81 (1)°. The two phenyl rings make a dihedral angle of 65.13 (14)°. The structure exhibits inter­molecular hydrogen bonds of the types O—H⋯O and C—H⋯O

Synthesis and Crystal Structure of 1-Benzhydryl-4-Methane-Sulfonyl-Piperazine

The title compound, 1-benzhydryl-4-methanesulfonyl-piperazine, was synthesized by the nucleophilic substitution of 1-benzhydryl-piperazine with methyl sulfonyl chloride. The product obtained was characterized by spectroscopic techniques, and the structure was investigated by X-ray crystallography. The compound crystallizes in the monoclinic crystal class in the space group P21/c with cell parameters a = 9.5820(4) A○, b = 16.8150(12) A○, c = 13.5280(8) A○, β = 127.270(5)°, and V = 1734.5(2)A○ 3 for Z = 4. The structure reveals that the piperazine ring is in a chair conformation. There is a large discrepancy around the bond angles of the piperazine N atoms. The geometry around the S atom is distorted tetrahedral

1-Phenyl\-sulfonyl-1\it H-1,2,4-triazole

In the title compound, C\sb 8H\sb 7N\sb 3O\sb 2S, the dihedral angle between the 1,2,4-triazole ring and the phenyl ring is 82.17(14)\circ. The geometry around the S atom is distorted tetra\-hedral. The mol\-ecules are linked by inter\-molecular C—-H⋅sN and C—-H⋅sO hydrogen bonds

Synthesis and in vitro antiproliferative activity of diphenyl(sulphonylpiperidin-4-yl)methanol derivatives

A series of novel diphenyl(piperidin-4-yl)methanol derivatives 10(a--n) were synthesized and characterized by 1H NMR, LC/MS, FTIR, and elemental analyses. All the synthesized compounds were evaluated for cell proliferation by MTT assay. The antiproliferative effects of the synthesized compounds were tested against viable human skin fibroblast cell line and carcinoma cell lines, namely HeLa cells, HT-29 cells, MCF-7 cells, and HepG-2 cells in comparing the positive and negative control. Among the synthesized compounds, (10b) and (10g) have been identified as potent antiproliferative agents