Schematic of an integrated clinical approach to understanding the impact of intratumour heterogeneity upon disease progression and clinical outcome.

<p>Abbreviations: cfDNA, circulating-free tumour DNA; CTCs, circulating tumour cells; FACS, fluorescence-activated cell sorting; FISH, fluorescence in situ hybridisation; IHC, immunohistochemistry. Lungs diagram adapted from “Lungs diagram simple” from Patrick J. Lynch, Wikimedia Commons under CY-BY 2.5. Metastatic disease image from Haubner, et al. (2005) PLoS Med 2: e70. doi:10.1371/journal.pmed.0020070. Images of FACS analysis, immunohistochemistry, and FISH obtained from the Swanton lab.</p