Short-Term Exposure to Nanoplastics Does Not Affect Bisphenol A Embryotoxicity to Marine Ascidian Ciona robusta

Plastic pollution is recognized as a global environmental threat and concern is increasing regarding the potential interactions of the smallest fragments, nanoplastics (1 m), with either physical and chemical entities encountered in the natural environment, including toxic pollutants. The smallest size of nanoplastics (<100 nm) rebounds to their safety associated with remarkable biological, chemical and physical reactivity that allow them to interact with cellular machinery by crossing biological barriers and causing damage to living beings. Recent findings on nanoplastic occurrence inmarine coastal waters, including the Mediterranean Sea, leave open the question on their ability to act as a vector of other contaminants of emerging concerns (CECs) concomitantly released by wastewater treatment plants and reaching marine coastal waters. Here, we assess for the first time the role of non-functionalized polystyrene nanoparticles (PS NPs, 20 nm) as a proxy for nanoplastics (1 and 10 g/mL) alone and in combinationwith bisphenolA(BPA) (4.5 and 10 m) on Ciona robusta embryos (22 h post fertilization, hpf) by looking at embryotoxicity through phenotypic alterations. We confirmed the ability of BPA to impact ascidian C. robusta embryo development, by affecting sensory organs pigmentation, either alone and in combination with PS NPs. Our findings suggest that no interactions are taking place between PS NPs and BPA in filtered sea water (FSW) probably due to the high ionic strength of seawater able to trigger the sorption surface properties of PS NPs. Further studies are needed to elucidate such peculiarities and define the risk posed by combined exposure to BPA and PS NPs in marine coastal waters

Sensitivity of Hydra vulgaris to Nanosilver for Environmental Applications

Nanosilver applications, including sensing and water treatment, have significantly increased in recent years, although safety for humans and the environment is still under debate. Here, we tested the environmental safety of a novel formulation of silver nanoparticles functionalized with citrate and L-cysteine (AgNPcitLcys) on freshwater cnidarian Hydra vulgaris as an emerging ecotoxicological model for the safety of engineered nanomaterials. AgNPcitLcys behavior was characterized by dynamic light scattering (DLS), while Ag release was measured by inductively coupled plasma mass spectrometry (ICP-MS). H. vulgaris (n = 12) subjects were evaluated for morphological aberration after 96 h of exposure and regeneration ability after 96 h and 7 days of exposure, after which the predatory ability was also assessed. The results show a low dissolution of AgNPcitLcys in Hydra medium (max 0.146% of nominal AgNPcitLcys concentration) and highlight a lack of ecotoxicological effects, both on morphology and regeneration, confirming the protective role of the double coating against AgNP biological effects. Predatory ability evaluation suggests a mild impairment of the entangling capacity or of the functionality of the tentacles, as the number of preys killed but not ingested was higher than the controls in all exposed animals. While their long-term sub-lethal effects still need to be further evaluated on H. vulgaris, AgNPcitLcys appears to be a promising tool for environmental applications, for instance, for water treatment and sensing

Eco-interactions of engineered nanomaterials in the marine environment: Towards an eco-design framework

Marine nano-ecotoxicology has emerged with the purpose to assess the environmental risks associated with engineered nanomaterials (ENMs) among contaminants of emerging concerns entering the marine environment. ENMs’ massive production and integration in everyday life applications, associated with their peculiar physical chemical features, including high biological reactivity, have imposed a pressing need to shed light on risk for humans and the environment. Environmental safety assessment, known as ecosafety, has thus become mandatory with the perspective to develop a more holistic exposure scenario and understand biological effects. Here, we review the current knowledge on behavior and impact of ENMs which end up in the marine environment. A focus on titanium dioxide (n-TiO2) and silver nanoparticles (AgNPs), among metal-based ENMs massively used in commercial products, and polymeric NPs as polystyrene (PS), largely adopted as proxy for nanoplastics, is made. ENMs eco-interactions with chemical molecules including (bio)natural ones and anthropogenic pollutants, forming eco- and bio-coronas and link with their uptake and toxicity in marine organisms are discussed. An ecologically based design strategy (eco-design) is proposed to support the development of new ENMs, including those for environmental applications (e.g., nanoremediation), by balancing their effectiveness with no associated risk for marine organisms and humans

The Seabed Makes the Dolphins: Physiographic Features Shape the Size and Structure of the Bottlenose Dolphin Geographical Units

The common bottlenose dolphin (Tursiops truncatus) is a cosmopolitan delphinid, regularly present in the Mediterranean Sea. According to previous studies, this dolphin tends to form resident geographical units scattered on the continental shelf. We investigated how the physiographic characteristics of the area of residence, with special reference to the size and shape of the continental shelf, affect the home range and the group size of the local units. We analysed and compared data collected between 2004-2016 by 15 research groups operating in different study areas of the Mediterranean Sea: the Alboran Sea, in the South-Western Mediterranean, the Gulf of Lion and the Pelagos Sanctuary for the marine mammals, in the North-Western Mediterranean, and the Gulf of Ambracia, in the North-Central Mediterranean Sea. We have found that in areas characterised by a wide continental platform, dolphins have wider home ranges and aggregate into larger groups. In areas characterized by a narrow continental platform, dolphins show much smaller home ranges and aggregate into smaller groups. The results obtained from this collective research effort highlight the importance of data sharing to improve our scientific knowledge in the field of cetaceans and beyond

Cost-Effectiveness Analysis for a Heavily Modified Water Body (HMWB): The Lambro-Seveso-Olona System Case Study

The Lambro-Seveso-Olona system (L-S-O) is a effluent dominated watershed which derives from a strong urbanization process of natural river basins. The average population density in the L-S-O area is among the highest in Italy and Europe. Industry is also highly developed. Although at present the L-S-O system does not receive untreated wastewaters, depurated effluents constitute about half of its streamflow. This river has a long history of poor quality status, due to the high concentration of pollutant loads and the poor dilution. Recently new chemical quality standards have been set by the Italian legislation as support for the WFD Good Ecological Status. These standards are very restrictive, and make extremely challenging the achievement of the good ecological status. Aim of this study is to analyse the restoration possibilities of the L-S-O system. Elements are provided for a Cost-Effectiveness analysis

Silver Nanoparticles for Water Pollution Monitoring and Treatments: Ecosafety Challenge and Cellulose-Based Hybrids Solution

Silver nanoparticles (AgNPs) are widely used as engineered nanomaterials (ENMs) in many advanced nanotechnologies, due to their versatile, easy and cheap preparations combined with peculiar chemical-physical properties. Their increased production and integration in environmental applications including water treatment raise concerns for their impact on humans and the environment. An eco-design strategy that makes it possible to combine the best material performances with no risk for the natural ecosystems and living beings has been recently proposed. This review envisages potential hybrid solutions of AgNPs for water pollution monitoring and remediation to satisfy their successful, environmentally safe (ecosafe) application. Being extremely efficient in pollutants sensing and degradation, their ecosafe application can be achieved in combination with polymeric-based materials, especially with cellulose, by following an eco-design approach. In fact, (AgNPs)–cellulose hybrids have the double advantage of being easily produced using recycled material, with low costs and possible reuse, and of being ecosafe, if properly designed. An updated view of the use and prospects of these advanced hybrids AgNP-based materials is provided, which will surely speed their environmental application with consequent significant economic and environmental impact

Progress in selecting marine bioindicators for nanoplastics ecological risk assessment

Nanoplastic (50 mgL−1). Both negligible impacts and detrimental effects, although poorly descriptive of the real environmental exposure scenarios, have been documented on different trophic levels and ecological functionalities. Polystyrene nanospheres (<100 nm) are by far the most tested as a proxy for nanoplastics, even though the occurrence of nanoplastics composed by other polymers and shapes (i.e., irregular and fibers) has been reported in seawater column and sediments. Limited information on bioaccumulation in marine species hamper the selection of key bioindicator species following various criteria (i.e., target, highly sensitive, endangered, etc) for pollution monitoring and ecological risk assessment (ERA) purposes. A holistic approach is thus required starting from setting concentrations as environmentally relevant coupled with chronic exposure, and selecting bioindicators including those having a key role in marine ecosystem processes, functions and services, also relevant for human consumption (shellfish and seafood). The present mini-review aims to provide a framework for the selection of the best bioindicators for nanoplastic in the marine environment along with current knowledge on sources, circulation and behavior in temperate and polar environments and potential compartments/species more at risk of exposure, to support nanoplastic ERA. Less investigated ecological niches and habitats, which should deserve more attention in future studies, are also identified

IL MAGISTERO DELLE VOLTE IN FOGLIO NELLA TRADIZIONE ROMANA: DAI RECUPERI IN ETÀ POST-INDUSTRIALE AL CANTIERE SPERIMENTALE

La tecnologia delle volte in foglio in laterizio, di antica origine mediterranea, impiegate dal tardomedioevo a Valencia e in Catalogna, conosce una lunga storia di migrazioni e contaminazioni culturali, fino all’età moderna e contemporanea. Note le applicazioni nelle regioni rivierasche sotto l’influenza politica e culturale spagnola in età moderna, come quelle in area europea in età post-industriale e nella straordinaria operazione di esportazione e innovazione tecnologica operata dal valenciano Rafael Guastavino (1842-1908) e dai suoi discendenti negli Stati Uniti, a partire dal 1881. Ancora poco esplorato è l’impiego, pur massiccio, di questa tecnologia rapida, economica e resistente al fuoco, nei cantieri di Roma capitale, tra fine ‘800 e secondo dopoguerra. In questo contesto di ricerca si è inserito il cantiere sperimentale di costruzione di una volta in foglio, realizzato presso la sede dell’ex Mattatoio del Dipartimento di Architettura di Roma Tre, contestualmente al convegno “Laterizio Romano” III, nel marzo 2019. La volta a vela, riproduzione in scala reale di un modello di Valencia, è stata progettata dagli studenti di Roma Tre e realizzata da due capaci voltaroli valenciani, con il contributo dei partecipanti al workshop e l’impiego di laterizi sperimentali prodotti ad hoc da una fornace artigiana dell’alto Lazio.Thin-tile vaulting, a traditional technique with ancient Mediterranean origins, which has been used since the late medieval times in Valencia and Catalunia, has a long history of migrations and cultural influences all the way up to its use in modern periods and the present day. The use of the technique in the coastal regions under Spanish political and cultural influences, during the modern age, is well-known, as well as its applications during the post-industrial times in Europe and its extraordinary export and technological innovation operated by the Valencian Rafael Guastavino (1842–1908) and his descendants in the United States since 1881. The use of the technique in the constructions sites of Rome Capital is still barely explored even though it played a major role in this area during the 1800s and after the second world war due to it being a fast, economical, and fireproof building style. Part of the context for this research is the experimental construction site of the thin-tile vault, realized at the Ex Mattatoio, the seat of the Department of Architecture of Roma Tre, simultaneously with the III “Laterizio Romano” conference, in March 2019. The sail vault, which is a real scale reproduction of a model in Valencia, has been designed by students of Roma Tre and realized by two skilled master builders with the help of the participants of the workshop, using experimental bricks made ad hoc by an artisan furnace of the Nothern Lazio

Preliminary assessment of environmental safety (ecosafety) of dextrin-based nanosponges for environmental applications

The ability to employ waste products, such as vegetable scraps, as raw materials for the synthesis of new promising adsorbing materials is at the base of the circular economy and end of waste concepts. Dextrin-based nanosponges (D_NS), both cyclodextrin (CD) and maltodextrin (MD), have shown remarkable adsorption abilities in the removal of toxic compounds from water and wastewater, thus representing a bio-based low-cost solution which is establishing itself in the market. Nevertheless, their environmental safety for either aquatic or terrestrial organisms has been overlooked, raising concern in terms of potential hazards to natural ecosystems. Here, the environmental safety (ecosafety) of six newly synthesized batches of D_NS was determined along with their full characterization by means of dynamic light scattering (DLS), thermogravimetric analysis (TGA), Fourier transformed infrared spectroscopy with attenuated total reflection (FTIR-ATR) and transmission electron microscopy (SEM). Ecotoxicity evaluation was performed using a battery of model organisms and ecotoxicity assays, such as the microalgae growth inhibition test using the freshwater Raphidocelis subcapitata and the marine diatom Dunaliella tertiolecta, regeneration assay using the freshwater cnidarian Hydra vulgaris and immobilization assay with the marine brine shrimp Artemia franciscana. Impact on seedling germination of a terrestrial plant of commercial interest, Cucurbita pepo was also investigated. Ecotoxicity data showed mild to low toxicity of the six batches, up to 1 mg/mL, in the following order: R. subcapitata > H. vulgaris > D. tertiolecta > A. franciscana > C. pepo. The only exception was represented by one batch (NS-Q+_BDE_(GLU2) which resulted highly toxic for both freshwater species, R. subcapitata and H. vulgaris. Those criticalities were solved with the synthesis of a fresh new batch and were hence attributed to the single synthesis and not to the specific D_NS formulation. No effect on germination of pumpkin but rather more a stimulative effect was observed. To our knowledge this is the first evaluation of the environmental safety of D_ NS. As such we emphasize that current formulations and exposure levels in the range of mg/mL do not harm aquatic and terrestrial species thus representing an ecosafe solution also for environmental applications

Remediation of acid mine drainage-affected stream waters by means of ecofriendly magnetic hydrogels crosslinked with functionalized magnetite nanoparticles

Acid mine drainage (AMD) is a global environmental challenge that represents a primary source of contamination of heavy metals in both active and abandoned mining areas. The present study explored the suitability of a carboxymethylcellulose (CMC) hydrogel cross-linked with functionalized magnetite (Fe3O4) nanoparticles, (CMC-Fe3O4), for the removal of heavy metals from AMD-affected stream waters and tested its eco-friendly nature. AMD-affected stream waters, collected in a former mining site located in the Metalliferous Hills ore district (Central Italy), were chosen as a suitable benchmark for validating the CMC-Fe3O4 hydrogel applications. The remediation capability of the CMC-Fe3O4 hydrogel was investigated on solutions of Zn(II) in deionized (DI) water and on samples of AMD-affected stream waters. The CMC-Fe3O4 hydrogel was able to adsorb Zn(II) from DI water solutions as well as to reduce significantly the concentration of Zn and other heavy metals such as Cd, Co, Cu, Ni and Pb, in AMD-affected stream waters. CMC-Fe3O4 hydrogel and treated waters (both Zn-solution and AMD-affected stream waters) were tested for their effects on the freshwater green alga R. subcapitata by using 72-h growth inhibition tests. No effects were observed on the R. subcapitata growth upon exposure to CMC-Fe3O4 hydrogel, suggesting its eco-friendly nature. Moreover, CMC-Fe3O4 hydrogel was able to reduce the toxicity of heavy metals to the green alga, both in DI and AMD-affected stream water. Based on our findings, CMC-Fe3O4 hydrogel could be considered as a valuable tool for an environmentally safe treatment of AMD-affected stream waters