Boat electrofishing survey of common smelt and common bullies in the Ohau Channel

We conducted a boat electrofishing survey of the Ohau Channel, which flows from Lake Rotorua to Lake Rotoiti, on 13 December 2007. The purpose of the survey was to investigate the longitudinal pattern in densities of common smelt (Retropinna retropinna) and common bullies (Gobiomorphus cotidianus) along the Ohau Channel. We caught 1,267 fish comprising three native fish species and two introduced fish species in 1.58 km of fished distance at a total of 10 sites. Native species caught were the common smelt, common bully and longfinned eel (Anguilla dieffenbachii) and introduced species were rainbow trout (Oncorhynchus mykiss) and goldfish (Carassius auratus). Assuming that the bow-mounted anodes effectively fished a 4 m swath then the total area fished was 6,328 m2 (0.632 ha). Common smelt densities varied among the 10 different sites in the Ohau Channel ranging from 0 to 10.6 fish 100 m-2. Smelt density was higher at the upstream end of the channel near the weir at the Lake Rotorua outlet, decreasing with increasing distance from the weir. Smelt were found in the littoral zones but were not caught in mid-channel habitats. In the upstream reaches of the Ohau Channel, directly below the weir, a high number of juveniles (4.4 fish 100 m-2) were captured compared to the amount of juveniles captured at the other sites (0 – 1.2 fish 100 m-2). Common bully densities varied among the 10 different sites in the Ohau Channel ranging from 0.2 to 58.3 fish 100 m-2. No longitudinal pattern in the distribution of common bullies was evident along the channel. The highest densities were found halfway along the Ohau Channel where there was an abundance of dense macrophyte beds. Common bully densities were found to be much higher in the edge habitats with macrophyte beds compared to the mid-channel habitats and the willow edge habitat where there were relatively low densities. Size frequency data shows that there is generally a higher proportion of small bullies than larger ones suggesting that recruitment is occurring. Both adult and juvenile rainbow trout were observed in the Ohau Channel. Most of these individuals were found in the upstream section of the channel below the weir and ranged from a 75 mm juvenile to a fully grown adult about 500 mm long. Large longfinned eels were also captured and were only found in the downstream section of the Ohau Channel in willow-dominated edges. In the bottom third section of the channel, near the possible artificial embayment, goldfish were present

Boat electrofishing survey of the Awaiti and Bancrofts canals (Tee Head Canal), Piako River system

This purpose of this survey of Awaiti and Bancrofts canals was to establish the presence of fish in the canals and to determine their relative density and biomass by boat electrofishing. These canals drain the Kopuatai Peat Dome and adjacent farmland into Tee Head Canal, which is part of the Piako River system in the Waikato Region. Awaiti Canal had patches of the emergent macrophyte Eurasian reed sweet grass (Glyceria maxima) and floating common duckweed (Lemna minor). Bancrofts Canal had dense beds of reed sweet grass and water pepper (Persicaria hydropiper, syn. Polygonum hydropiper) that extended into the water along most of its margin to the extent that the bank was not visible. The water of both canals was tannin-stained, but Bancrofts Canal had heavier staining than Awaiti Canal. We boat electrofished five sites in Awaiti Canal and five sites in Bancrofts Canal for 10 mins each on 21 February 2018. Water temperature was 24.3°C in Awaiti Canal and 23.1°C in Bancrofts Canal. In Awaiti Canal, ambient electrical conductivity was 313.5 µS cm⁻¹, specific conductivity was 317.6 µS cm⁻¹, and black disc distance was 0.18 m. In Bancrofts Canal, ambient conductivity was 174.3 µS cm⁻¹, and specific conductivity was 180.8 µS cm⁻¹. We caught 31 fish in total, 28 in Awaiti Canal and 3 in Bancrofts Canal. Species included shortfin eels (Anguilla australis), inanga (Galaxias maculatus), goldfish (Carassius auratus), brown bullhead catfish (Ameiurus nebulosus) and gambusia (Gambusia affinis). Two inanga at site 4 (Awaiti Canal) were retrieved dead on capture, as was the one eel retrieved in Bancrofts Canal. Fish densities were very low, up to a maximum of 1.02 fish 100 m-2 for all species combined at site 3 in Awaiti Canal. Only gambusia were found alive in Bancrofts Canal. Biomass per site and areal biomass were similarly very low, and this was partly attributable to the small size of individuals, which suggested that, with the exception of gambusia, they were young, newly recruited fish. Awaiti Canal had a low diversity of small fish species that are generally tolerant of poor water quality. This fish diversity is particularly low considering the close proximity of the sites to the coast (about 30 km from the Firth of Thames). The most diverse fish communities in New Zealand are found close to the coast. Typical mean eel densities in the Waikato River assessed by boat electrofishing were 0.7-1.3 eels 100 m⁻² compared to the mean of 0.12 fish 100 m⁻² (range of 0-0.29 eels 100 m⁻² ) at the five sites Awaiti Canal. Bancroft Canal had only gambusia, and the one shortfin eel found dead could have drifted in or died in the canal, probably as result of low DO concentrations

Boat electrofishing survey of fish abundance in the Ohau Channel, Rotorua, in 2015

The aim of the survey was to provide on-going monitoring of the fish communities and abundance by boat electrofishing in the Ohau Channel, especially fish species that are taonga to Maori (eels, goldfish, and koura). In the current study, we present the findings from the ninth year of sampling (2015) and a summary of previous surveys. We used the University of Waikato's 4.5 m-long, aluminium-hulled electrofishing boat to catch a total of total of 1,198 fish and koura (18.9 kg) at 13 sites on 2 December 2015, which comprised 2,671 lineal m and 10,684 m² in area. Koura (freshwater crayfish) and 6 fish species were present, with common bully the most abundant species (up to 45.5 fish 100 m⁻² at the site 6, edge habitat). Goldfish (up to 4.85 100 m⁻²) was the next most abundant species, with most goldfish at sites 7 and 12-13 in and around the side channel. Rainbow trout densities were up to 0.28 fish 100 m⁻². Mean bully density (11.41 fish 100 m⁻²) was much higher than for smelt (0.29 fish 100 m⁻²). Koura had a patchy distribution; only 3 individuals were caught at one site. Comparing catches over the 9 years of sampling, the mean abundance of common bullies in 2015 was consistent with densities in most post-wall years (after 2007), but lower than in 2007 before wall closure (ANOVA P = 0.001). The cause of fluctuating bully abundance is not known, and was not accounted for by changes in water clarity expressed as black disc distance (BDD), water temperature, or water conductivity. Poor water clarity can reduce the efficiency of electrofishing, but high BDD did not correspond with high common bully densities. In 2015, smelt abundance had recovered somewhat from the low catch in 2014. Goldfish biomass increased initially (2009-2010) because of targeted fishing in the excavated side branch (site 11), which has dense macrophytes and offers good habitat for goldfish. The continued rise in density from 2012 on, however, suggests a real increase in goldfish numbers. In 2012 and 2013 shortfin eels were caught, but no eels were caught in 2014 and only a single longfin eel was caught in 2015. Analysis of fish densities before and after wall closure is hampered by the single data point before closure. However, we now have 9 years of post-wall data, and comparison of means suggest that the number of bullies has been lower since 2007. An obvious cause could be interruption of bully migration into the Ohau Channel from Lake Rotoiti by the wall. This suggests that the bully population in the Ohau Channel before wall construction and closure was a mixture of fish from lakes Rotorua and Rotoiti, and that recruitment from Rotoiti is now restricted. This hypothesis is testable with otolith microchemistry

Boat electrofishing survey of fish abundance in the Ohau Channel, Rotorua, in 2017

The aim of the survey was to provide on-going monitoring of the fish communities and abundance by boat electrofishing in the Ohau Channel, especially fish species that are taonga to Maori (tuna, or eels, morihana, or goldfish, and kōura, or freshwater crayfish). In the current study, we present the findings from the eleventh year of sampling (2017) and a summary of previous surveys. We used the University of Waikato’s 4.5 m-long, aluminium-hulled electrofishing boat to catch a total of 1,110 fish and 3 kōura (18.2 kg in total) from 11 sites on 5 December 2017. The sites comprised 2,909 lineal m and 11,636 m2 in area. Kōura (freshwater crayfish) and 6 fish species were present, with common bully the most abundant species (up to 29.6 fish 100 m⁻² at the site 8, edge habitat). Goldfish (up to 14.2 100 m⁻²) was the next most abundant species, with most goldfish at sites 8 and 11 in and around the side channel. Common smelt were next the most abundant species (up to 11.4 fish 100 m⁻²). Mean density of bullies (7.8 fish 100 m⁻²) was much higher than for smelt (1.69 fish 100 m⁻²). Kōura had a patchy distribution with only 3 individuals were caught at two sites. Comparing catches over the 11 years of sampling, the mean abundance of common bullies in 2017 was consistent with densities in most post-wall years (after 2007), but lower than in 2007 before wall closure (Newman-Keuls multiple range test, P = 0.034). The cause of fluctuating bully abundance is not known, and was not accounted for by changes in water clarity expressed as black disc distance (BDD), water temperature, or water conductivity. Poor water clarity can reduce the efficiency of electrofishing, but low BDD did not correspond with low common bully densities. In 2017, smelt abundance had recovered somewhat from the low catches from 2014 to 2016. Goldfish biomass increased initially (2009-2010) because of targeted fishing in the side channel (site 11), which has dense macrophytes and offers good habitat for goldfish with no flow. However, the continued rise in goldfish density from 2012 on suggests a real increase in goldfish numbers. In contrast to most previous years, no eels were caught in 2017. Analysis of fish densities before and after wall closure is hampered by the single data point before closure. However, we now have 10 years of post-wall data, and comparisons of means and standard deviations suggest that the number of bullies have been lower since 2008 with the exception of 2015, when bully densities were the same as 2007, i.e., before wall closure. The large number of small juvenile bullies (<30 mm) suggests that recruitment is taking place in the channel, so fluctuating bully abundance is likely to be controlled by factors other than wall closure

Boat electrofishing survey of fish abundance in the Ohau Channel, Rotorua, in 2016

The aim of the survey was to provide on-going monitoring of the fish communities and abundance by boat electrofishing in the Ohau Channel, especially fish species that are taonga to Maori (tuna, or eels, morihana, or goldfish, and kōura, or freshwater crayfish). In the current study, we present the findings from the tenth year of sampling (2016) and a summary of previous surveys

Comparing plasma and faecal measures of steroid hormones in Adelie penguins Pygoscelis adeliae

Physiological measurements of both stress and sex hormones are often used to estimate the consequences of natural or human-induced change in ecological studies of various animals. Different methods of hormone measurement exist, potentially explaining variation in results across studies; methods should be cross-validated to ensure that they correlate. We directly compared faecal and plasma hormone measurements for the first time in a wild free-living species, the Adelie penguin (Pygoscelis adeliae). Blood and faecal samples were simultaneously collected from individual penguins for comparison and assayed for testosterone and corticosterone (or their metabolites). Sex differences and variability within each measure, and correlation of values across measures were compared. For both hormones, plasma samples showed greater variation than faecal samples. Males had higher mean corticosterone concentrations than females, but the difference was only statistically significant in faecal samples. Plasma testosterone, but not faecal testosterone, was significantly higher in males than females. Correlation between sample types was poor overall, and weaker in females than in males, perhaps because measures from plasma represent hormones that are both free and bound to globulins, whereas measures from faeces represent only the free portion. Faecal samples also represent a cumulative measure of hormones over time, as opposed to a plasma ‘snapshot’ concentration. Our data indicate that faecal sampling appears more suitable for assessing baseline hormone concentrations, whilst plasma sampling may best define immediate responses to environmental events. Consequently, future studies should ensure that they select the most appropriate matrix and method of hormone measurement to answer their research questions

Evolution of Competitive Ability: An Adaptation Speed vs. Accuracy Tradeoff Rooted in Gene Network Size

Ecologists have increasingly come to understand that evolutionary change on short time-scales can alter ecological dynamics (and vice-versa), and this idea is being incorporated into community ecology research programs. Previous research has suggested that the size and topology of the gene network underlying a quantitative trait should constrain or facilitate adaptation and thereby alter population dynamics. Here, I consider a scenario in which two species with different genetic architectures compete and evolve in fluctuating environments. An important trade-off emerges between adaptive accuracy and adaptive speed, driven by the size of the gene network underlying the ecologically-critical trait and the rate of environmental change. Smaller, scale-free networks confer a competitive advantage in rapidly-changing environments, but larger networks permit increased adaptive accuracy when environmental change is sufficiently slow to allow a species time to adapt. As the differences in network characteristics increase, the time-to-resolution of competition decreases. These results augment and refine previous conclusions about the ecological implications of the genetic architecture of quantitative traits, emphasizing a role of adaptive accuracy. Along with previous work, in particular that considering the role of gene network connectivity, these results provide a set of expectations for what we may observe as the field of ecological genomics develops

The Role of Spatially Controlled Cell Proliferation in Limb Bud Morphogenesis

Oriented cell behaviors likely have a more important role in limb bud elongation during development than previously suggested by the “growth-based morphogenesis” hypothesis

High fat diet enhances stemness and tumorigenicity of intestinal progenitors

Little is known about how pro-obesity diets regulate tissue stem and progenitor cell function. Here we find that high fat diet (HFD)-induced obesity augments the numbers and function of Lgr5+ intestinal stem-cells (ISCs) of the mammalian intestine. Mechanistically, HFD induces a robust peroxisome proliferator-activated receptor delta (PPAR-d) signature in intestinal stem and (non-ISC) progenitor cells, and pharmacologic activation of PPAR-d recapitulates the effects of a HFD on these cells. Like a HFD, ex vivo treatment of intestinal organoid cultures with fatty acid constituents of the HFD enhances the self-renewal potential of these organoid bodies in a PPAR-d dependent manner. Interestingly, HFD- and agonist-activated PPAR-d signaling endow organoid-initiating capacity to progenitors, and enforced PPAR-d signaling permits these progenitors to form in vivo tumors upon loss of the tumor suppressor Apc. These findings highlight how diet-modulated PPAR-d activation alters not only the function of intestinal stem and progenitor cells, but also their capacity to initiate tumors

Prognostic model to predict postoperative acute kidney injury in patients undergoing major gastrointestinal surgery based on a national prospective observational cohort study.

Background: Acute illness, existing co-morbidities and surgical stress response can all contribute to postoperative acute kidney injury (AKI) in patients undergoing major gastrointestinal surgery. The aim of this study was prospectively to develop a pragmatic prognostic model to stratify patients according to risk of developing AKI after major gastrointestinal surgery. Methods: This prospective multicentre cohort study included consecutive adults undergoing elective or emergency gastrointestinal resection, liver resection or stoma reversal in 2-week blocks over a continuous 3-month period. The primary outcome was the rate of AKI within 7 days of surgery. Bootstrap stability was used to select clinically plausible risk factors into the model. Internal model validation was carried out by bootstrap validation. Results: A total of 4544 patients were included across 173 centres in the UK and Ireland. The overall rate of AKI was 14·2 per cent (646 of 4544) and the 30-day mortality rate was 1·8 per cent (84 of 4544). Stage 1 AKI was significantly associated with 30-day mortality (unadjusted odds ratio 7·61, 95 per cent c.i. 4·49 to 12·90; P < 0·001), with increasing odds of death with each AKI stage. Six variables were selected for inclusion in the prognostic model: age, sex, ASA grade, preoperative estimated glomerular filtration rate, planned open surgery and preoperative use of either an angiotensin-converting enzyme inhibitor or an angiotensin receptor blocker. Internal validation demonstrated good model discrimination (c-statistic 0·65). Discussion: Following major gastrointestinal surgery, AKI occurred in one in seven patients. This preoperative prognostic model identified patients at high risk of postoperative AKI. Validation in an independent data set is required to ensure generalizability