RAPD profiles of representative circulating Venezuelan <i>Trypanosoma cruzi</i> strains.

<p>Loading order, left to right <b>A</b>: 1, CanIII cl 1 (TcIV); 2, Esmeraldo cl2 (TcII); 3, WA250 cl 10B (TcI); 4, 8839(TcIV); 5, 8196(TcIV); 6, 10141(TcI); 7, 10610(TcIV); 8, 8089(TcI); 9, 6872(TcI); 10, PGN23(TcI); 11, PGN27(TcIV); 12, PGN31(TcI); L, 1 kb DNA Ladder. <b>B</b>: 1, CanIII cl1 ; 2, Esmeraldo cl2; 3, WA250 cl 10B; 4, 11932; 5, 7082; 6, 8104; 7, 7570; 8, 7780; 9, SJ1097; 10, pgn2; 11, PGCHG; 12, CD45.</p

Reference strains used in this study.

<p>Reference strains used in this study.</p

<i>Trypanosoma cruzi</i> genotypes from different host species in Venezuela.

<p><i>Trypanosoma cruzi</i> genotypes from different host species in Venezuela.</p

Oligonucleotide primers employed in this study.

<p>Oligonucleotide primers employed in this study.</p

PCR-RFLP genotyping profiles of Venezuelan <i>Trypanosoma cruzi</i> strains.

<p>Each pair of lanes shows undigested PCR product followed by restriction digest products for GPI (A) and HSP60 (B). Loading order, left to right: 1, BAJV104; 2, XPMPDM5; 3, VE1003; 4, VE3303; 5, BACR104; 6, BAJT104; 7, PARAMA13; L(A), Hyperladder I (Bioline, UK); L(B), Hyperladder V (Bioline, UK).</p

Isoenzyme profiles of GPI and PGM from representative circulating Venezuelan <i>Trypanosoma cruzi</i> strains.

<p>Loading order, left to right: TcI, WA250 cl 10B; TcII, Esmeraldo cl2; TcIV, CanIII cl 1; 1, 8839(TcIV); 2, 8196(TcIV); 3, 10141(TcI); 4, 10610(TcIV); 5, 8089(TcI); 6, 6872(TcI); 7, PGN23(TcI); 8, PGN27(TcIV); 9, PGN31(TcI).</p

Distribution of <i>Trypanosoma cruzi</i> Discrete Typing Units (DTUs) in Venezuela.

<p>The three maps display samples collected from humans (top left), vectors (top right), and mammal reservoirs (bottom left). Pie chart area is proportional to sampling size. Pie segment colour represents DTU identity. Inset on each map shows the capital (Caracas) and surrounding states in greater detail.</p

Geographical distribution of <i>Trypanosoma cruzi</i> genotypes from different hosts in Venezuela.

**<p>Morphological and molecular identification.</p>*<p>Morphological identification only.</p

Summary list of <i>Trypanosoma cruzi</i> genotypes by host in Venezuela.

<p>Summary list of <i>Trypanosoma cruzi</i> genotypes by host in Venezuela.</p