Comparison of osteoblast cells growth in flat and long bones in mice and rabbits

Introduction: Several in vitro studies have shown the effect of external stimulus on acceleration of long bone fracture repair in experimental animals. The aim of this study was to determine feasibility and creation of appropriate conditions for proliferation of the bone cells, in flat and long bones of mice and rabbits . Materials and Methods: Mice and rabbits flat and long bones were cultured in 12 plates. At 14th day of culture, they reached an appropriate level of cell growth. Then specific staining methods were used to study the pattern of cell growth. Results: The results showed a significant differences (P<0.05) in the number of proliferated flat bone (Calvaria) and long bone (Tibia) of rabbits in the first, second and third weeks of cell cultures. The highest rate of growth was observed in the third week in both bones. Similar differences were found in mice. The highest rate of growth in calvaria and tibial bones of mice were found in the second and third weeks, respectively. Conclusion: It seems that growth and proliferation rate of flat bones in mice and rabbits spread more quickly than their lone bones. Thus, this point should be taken into account in the in vitro study of repair pattern of bone tissue

Outlining of high quality coking coal by concentration–volume fractal model and turning bands simulation in East-Parvadeh coal deposit, Central Iran

Artículo de publicación ISIThis study aims at identifying the proper parts of C1 and B2 coking coal seams in the North block of East-Parvadeh coal deposit (Central Iran) using the concentration–volume (C–V) fractal modeling according to sulfur and ash values which were calculated by turning bands conditional simulation. The C–V log–log plots were generated based on results of 100 realizations derived via turning bands simulation which show seven different geochemical populations for both sulfur and ash data in B2 seam which has a relatively good quality for coking coal with sulfur and ash values lower than 1.548% and 6.39% respectively. Additionally, C–V log–log plots indicate that there are seven and six for sulfur and ash geochemical populations in C1 seam containing a proper coal quality with respect to sulfur and ash values less than 1.41% and 6.92% respectively. High quality populations are located in the northern and western parts of the studied area which correlated with USGS standard. The logratio matrix was used for the correlation between results obtained by the C–V fractal modeling and geological particulars consisting of pyritic veins and ash coals. Based on the logratio matrix for sulfur values higher than 3.55% and 3.39% for C1 and B2, respectively, low quality parts of the seams have good correlation with pyritic veins in the eastern and central parts of the area. Moreover, there are high values of overall accuracy (OA) for correlation between parts of the seams with high values of ash which are 47.86% and 39.81% for C1 and B2, respectively, and ash coals obtained by geological data