Effect of Saliva Contamination on Microleakage of Open Sandwich Restorations

Svrha istraživanja: Željela se procijeniti mikropropusnost konvencionalnih staklenih ionomera, smolom modificiranih staklenih ionomera i hibridnih staklenih ionomera u otvorenim restauracijama II. razreda kontaminiranih slinom ili nekontamitiranih slinom. Materijal i metode: Korišteno je 60 ekstrahiranih zdravih ljudskih kutnjaka i preparirano je 120 kaviteta II. razreda na mezijalnim i distalnim plohama. Rubovi gingive nalazili su se 1 mm ispod caklinsko-cementnog spojišta. Svi uzorci nasumično su podijeljeni u četiri skupine (n = 15): skupina I – visoko viskozni stakleni ionomer (Fuji IX GP); skupina II – smolom modificirani stakleni ionomer (Fuji II LC); skupina III – hibridni stakleni ionomer (Equia-fil Forte); skupina IV – kompozitna smola (G’aenial Posterior). U skupini s otvorenim sen-dvič-restauracijama staklenoionomerni materijali postavljeni su na gingivno dno u debljini od 1 mm, a ostatak kaviteta ispunjen je kompozitnim materijalom. Nakon izrade restauracije na mezijalnim plohama, distalni kaviteti restaurirani su prema istom protokolu nakon kontaminacije slinom. Uzorci su podvrgnuti termocikliranju od 10 000 ciklusa na temperaturi od 5 °C do 55 °C i uronjeni tijekom 24 sata u otopinu metilenskog modrila (% 0,5). Zatim su se prerezani dijamantnom pilom, uz vodeno hlađenje, vertikalno kroz središte restauracije od mezijalne plohe prema distalnoj na debljinu od 1 mm. Nakon toga je prodiranje boje procjenjivano softverom za analizu slika. Podatci su statistički analizirani (p 0,05). Uz to, nije bilo statistički značajne razlike između skupina kontaminiranih slinom i nekontaminiranih slinom, neovisno o testiranim dentalnim materijalima (p > 0,05). Zaključak: Uzimajući u obzir ograničenja ovog istraživanja, u otvorenim sendvič-restauracijama kontaminacija slinom nije štetno utjecala na mikropropuštanje, bez obzira na testirane dentalne materijale. Hibridni stakleni ionomeri i stakleni ionomeri modificirani smolom pokazali su lošije rezultate u mikropropusnosti na gingivnim rubovima u odnosu prema visokoviskoznim staklenoionomernim materijaloma u skupini bez kontaminacije.Objectives: The purpose of the present study was to evaluate the microleakage of conventional glass-ionomer, resin modified glass-ionomer and glass hybrid ionomer Class II open sandwich restorations with or without saliva contamination. Material and methods: Sixty extracted sound human molar teeth were used and 120 class II slot cavities were prepared in mesial and distal surfaces. The gingival margins were located 1 mm below the cementoenamel junction. All specimens were randomly divided in 4 groups (n=15): Group I: High-Viscous Glass Ionomer (Fuji IX GP) Group II: Resin Modified Glass Ion-omer (Fuji II LC) Group III: Glass Hybrid Ionomer (Equia-fil Forte), Group IV: Composite Resin (G’aenial Posterior). In open sandwich restoration groups, glass ionomer materials were placed to gingival floor in 1 mm thickness and rest of the cavity was filled with resin composite. After the restorations in mesial surfaces had been performed, distal cavities were restored with the same protocol after saliva contamination. The specimens were thermocycled for 10000 cycles at 5 0 C to 55 0 C and immersed in methylene blue dye solution (% 0,5) for 24 hours. Then, they were sectioned vertically through the center of the restorations from mesial to distal surface with a water-cooled diamond saw with 1mm thickness. Subsequently, the dye penetration was evaluated with image analysis software. Data were statistically analyzed (p0.05). Additionally, there was not a statistically significant difference between the no contamination and saliva contaminated groups regardless of dental materials tested (p>0.05). Conclusion: Within the limitations of this study, in open sandwich restorations, saliva contamination did not show an adverse effect on microleakage irrespective of dental materials tested. Glass hybrid ionomers and resin modified glass ionomers showed lower microleakage scores in gingival margins compared to high-viscous glass ionomer material in no contamination groups