KLJUČNI POKAZATELJI USPJEŠNOSTI PROCESA ZAŠTITE ZDRAVLJA, SIGURNOSTI NA RADU I ZAŠTITE OKOLIŠA

U suvremenom poslovanju i upravljanju poslovnim procesima standardizacija svih postupaka omogućava stvaranje do-datne vrijednosti, konkurentnosti i uspješnosti u poslovanju organizacije. U tom smislu primjena ključnih pokazatelja uspješnosti za upravljanje zaštitom zdravlja, sigurnošću na radu i zaštitom okoliša (HSE) pridonosi razvoju učinkovitih i djelotvornih modela upravljanja područjem HSE. U radu je prikazana analiza teorijskih postavki zaštite zdravlja, si-gurnosti na radu, zaštite okoliša te ključnih pokazatelja uspješnosti (KPI). KPI pomažu organizaciji definirati i mjeriti napredak prema postavljenim organizacijskim ciljevima. Za učinkovito mjerenje KPI neophodno je postavljanje poslov-nih ciljeva. Ciljevi poslovanja moraju biti specifični, mjerljivi, ostvarivi, svrsishodni i vremenski određeni. Na temelju provedene analize prepoznate su mogućnosti na osnovu kojih su određeni KPI za procese HSE. Praćenje i mjerenje KPI u području zaštite zdravlja, sigurnosti na radu i zaštite okoliša provodi se temeljem postavljenih ciljeva, a direktno u-tječe na poboljšanje učinkovitosti i djelotvornosti HSE. Obzirom da je područje HSE jedan od pokazatelja uspješnog po-slovanja organizacije, možemo konstatirati da utvrđivanje i mjerenje KPI u području zaštite zdravlja, sigurnosti na ra-du i zaštite okoliša pomaže u postizanju poslovnih ciljeva organizacije

KLJUČNI POKAZATELJI USPJEŠNOSTI PROCESA ZAŠTITE ZDRAVLJA, SIGURNOSTI NA RADU I ZAŠTITE OKOLIŠA

U suvremenom poslovanju i upravljanju poslovnim procesima standardizacija svih postupaka omogućava stvaranje do-datne vrijednosti, konkurentnosti i uspješnosti u poslovanju organizacije. U tom smislu primjena ključnih pokazatelja uspješnosti za upravljanje zaštitom zdravlja, sigurnošću na radu i zaštitom okoliša (HSE) pridonosi razvoju učinkovitih i djelotvornih modela upravljanja područjem HSE. U radu je prikazana analiza teorijskih postavki zaštite zdravlja, si-gurnosti na radu, zaštite okoliša te ključnih pokazatelja uspješnosti (KPI). KPI pomažu organizaciji definirati i mjeriti napredak prema postavljenim organizacijskim ciljevima. Za učinkovito mjerenje KPI neophodno je postavljanje poslov-nih ciljeva. Ciljevi poslovanja moraju biti specifični, mjerljivi, ostvarivi, svrsishodni i vremenski određeni. Na temelju provedene analize prepoznate su mogućnosti na osnovu kojih su određeni KPI za procese HSE. Praćenje i mjerenje KPI u području zaštite zdravlja, sigurnosti na radu i zaštite okoliša provodi se temeljem postavljenih ciljeva, a direktno u-tječe na poboljšanje učinkovitosti i djelotvornosti HSE. Obzirom da je područje HSE jedan od pokazatelja uspješnog po-slovanja organizacije, možemo konstatirati da utvrđivanje i mjerenje KPI u području zaštite zdravlja, sigurnosti na ra-du i zaštite okoliša pomaže u postizanju poslovnih ciljeva organizacije

Measles situation in Serbia in an era of measles elimination (2007-2009)

Following the introduction of measles immunization in Serbia in 1971, measles outbreaks were recorded every 3 to 5 years until 1997. The outbreak in 1997 with 4000 cases was the last large outbreak in Serbia. In 2007, an outbreak with 191 laboratory confirmed or epidemiologically linked cases was reported in Vojvodina. In 2008 and 2009, only 3 cases were confirmed. From 2007-2009, measles infections were most frequently detected in the Roma population but also in non-immunized or partially immunized persons from the general population

Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate Lactobacillus paracasei subsp paracasei

The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN14, containing divergently orientated P-prtP and P-prtM promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of P-prtM in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas P-prtP was completely inactive. When L. casei ATCC393(T) was used as host, both P-prtP and P-prtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different

Analysis of the presence of prtR proteinase gene in natural isolates of Lactobacillus rhamnosus

The region of the prtR gene coding for the active site of PrtR proteinase was detected in natural isolates of lactobacilli, previously determined as Lactobacillus rhamnosus. This region was present in all L. rhamnosus strains with proteolytic activity. The PCR primers used were constructed on the basis of the sequence of the catalytic domain of the prtR proteinase gene. These primers generated in colony-PCR procedure specific 611-bp product with DNA from natural isolates of L. rhamnosus. No PCR amplifications using these primers were obtained for closely related bacteria of genus Lactobacillus, regardless of their proteolytic activity. In addition, these primers could be used singly or in multiplex PCR together with the Lactobacillus genus-specific primers. Compared with the other proteinases within the genus Lactobacillus (PrtP, PrtB and PrtH) which retained the activity in cell-free proteinase extracts, PrtR proteinase showed proteolytic activity only under in vivo conditions (whole cells of the producing strains)

Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes

In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer's patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1 beta, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination