Ct values generated from FMDV 3D rRT-PCR performed on sections of 14 separate LFDs and corresponding values generated for MagNA pure extracted RNA from the equivalent original epithelial suspensions in parallel.

<p>LP: Loading Pad; WS: Wicking strip; NB: Nitrocellulose below Ab Band (NB); Nitrocellulose Ab Band (AbB); Nitrocellulose above Ab band (NA). LFDs spanned four serotypes O (red dots) (LFDs TUR 8/1969, BAR 2/2008, KUW 2/2008, SAU 3/2008, ZAM 5/2008, HKN 10/2005, IRN 53/2006, UKG 7B/2007, A (blue dots) (LFDs BAR 4/2009, IRN 1/2008, KEN 8/2008, TUR 20/2006, IRN 36/2007), Asia 1 (grey dots) (IRN 15/2001) and SAT 1 (yellow dots) (ZAM 5/2008).</p

Ct values generated from FMDV 3D rRT-PCR performed on sections of 5 separate LFDs and MagNA pure extracted RNA from the original epithelial suspensions and recorded by time of LFD testing.

<p>LP: Loading Pad; WS: Wicking strip; NB: Nitrocellulose below Ab Band (NB); Nitrocellulose Ab Band (AbB); Nitrocellulose above Ab band (NA). LFDs spanned two serotypes O (red dots) (LFDs HKN 10/2005, UKG 7B/2007) and A (blue dots) (LFDs TUR 20/2006, IRN53/2006, IRN 36/2007).</p

Visualization of the 24 overlapping PCR products representing the complete FMDV genome generated from the loading pad (LP) from LFD loaded with UKG 7B/2007.

<p>1.8% agarose gel stained with ethidium bromide (0.2 µg per ml). Lane 1, S-fragment of the 5′UTR, Lanes 2–22 L fragment, Lanes 23 and 24, poly A fragment.</p

Box plot of Ct values generated from FMDV 3D rRT-PCR performed on sections of LFDs from serotype SAT 1 isolate (ZAM 5/2008) eluted at one month after LFD testing.

<p>LFDs had either been stored at room temperature (RoomT) (green bar) or 37°C (red bar). LP: Loading Pad; WS: Wicking strip; Nitrocellulose Ab Band (AbB). Dotted blue line represents baseline data (Ct values from LFDs washed on day one).</p

Foot-and-mouth disease virus samples used in the study.

<p>ES: Tongue epithelial suspension. CC: Cell culture supernatant.</p><p>Foot-and-mouth disease virus samples used in the study.</p

Ct values generated from FMDV 3D rRT-PCR performed on sections of LFDs in dilution series from serotype O isolate (BAR 2/2008) compared against the visual detection on the Ag LFD.

<p>LP: Loading Pad; WS: Wicking strip; Nitrocellulose Ab Band (AbB)(+)  =  genome detected; (-)  =  No Ct.</p

Antigen (Ag) ELISA results from electroporated BHK cells passed once and twice on BTY cells.

<p>LP: Loading Pad; WS: Wicking strip; NC: Nitrocellulose. +: Obvious CPE; +/-: Suspected CPE: -: No CPE. O  =  O serotype detected, A  =  A serotype detected, Asia 1  =  Asia 1 serotype detected. LP/WS: Loading Pad combined with Wicking Strip; NC: Nitrocellulose. (1)  =  LFDs washed the same day. (2)  =  LFDs washed one week later.</p><p>Antigen (Ag) ELISA results from electroporated BHK cells passed once and twice on BTY cells.</p