Microbiological Immunocytochemistry: A Review of Current Trends and Applications

It has been considered worthwhile to update previous reviews of microbiological immunocytochemistry in order to identify areas of current importance in this continually expanding area of research. Publications in virology, bacteriology and protozoology indicate a continued interest in immunocytochemistry. Deployment of colloidal gold techniques is almost universal in these applications. The post-embedding technique was the most widely applied technique although a few studies employed the immunonegative stain, pre-embedding and immunoreplica techniques, thereby reflecting the use of colloidal gold in all other areas of the biological sciences

Bioapplication of Colloidal Gold in Microbiological Immunocytochemistry

Microbiological organisms are an ubiquitous group of animals encompassing bacteria, viruses, protozoa, algae and fungi. They are adapted for survival in many diverse habitats and exert a profound effect on man and his environment. Colloidal gold electron immunocytochemistry is a useful technique for studying these organisms and may be applied in several ways. The post-embedding technique is used to detect internal antigens, whilst the pre-embedding technique is employed for the detection of external antigens. In contrast the immuno-negative stain technique is applied to detect antigens on structures such as viruses or bacterial pili which may be dried down onto an electron microscope grid and immunolabelled in situ. In addition the immuno-replica technique allows the examination of cell surfaces for the appearance of antigens. Together these techniques have yielded valuable information concerning microbiological organisms

Incomplete Transition Complexity of Basic Operations on Finite Languages

The state complexity of basic operations on finite languages (considering complete DFAs) has been in studied the literature. In this paper we study the incomplete (deterministic) state and transition complexity on finite languages of boolean operations, concatenation, star, and reversal. For all operations we give tight upper bounds for both description measures. We correct the published state complexity of concatenation for complete DFAs and provide a tight upper bound for the case when the right automaton is larger than the left one. For all binary operations the tightness is proved using family languages with a variable alphabet size. In general the operational complexities depend not only on the complexities of the operands but also on other refined measures.Comment: 13 page

Markets, Government And Environmental Policy Issues For Public Transit

This paper considers the wider transport policy implications of bus deregulation, especially the links with environmental objectives. The major themes are the role of markets in creating opportunities through incentives to innovate which impact positively on the environment without the intervention of government, but which accord with political agendas, defining an appropriate set of goals and performance criteria for urban passenger transport which give credence to environmental sustainability, distinguishing outcome and outputs and structuring the regulator to deliver. We use the experience with mini-buses in Britain to show how markets create environmentally compatible incentives

An evaluation of non-linear undrained behaviour in the moderate strain range for fine-grained soils

To select appropriate stress-strain parameters for serviceability limit state calculations, an understanding is needed of the likely variation of stress-strain behaviour within the model displacement mechanism. One approach that may be utilised to investigate variations in stress-strain behaviour is by employing a simple non-linear model (with a small number of physically significant parameters) that simulates experimental measurements of soil stress-strain with reasonable precision. By testing the sensitivity of the model parameters to changes in physical properties that can be expected to be related to them the reliability of different models can be established. Recently, empirical analysis of the published triaxial test database RFG/TXCU-278 identified a significant positive correlation between γ50 and OCR for four test modes (CIUC, CIUE, CKUC and CKUE). In this paper, a new experimental dataset from a programme of reconstituted soil tests on Kaolin and Bothkennar Clay is used to investigate the validity of a simple non-linear model

Comparison of simple stress-strain models in the moderate strain range for fine-grained soils:A review

The prediction of stress-strain behaviour in soils is a problem that can be approached in different ways depending on the design scenario. In some cases, a multi-parameter constitutive model calibrated with non-routine soil tests may be appropriate, for example, where a model has been developed for the relevant soil at a building site subject to complex loading. However, simple characteristic parameters are desirable for examining the variability of soil behaviour especially at regional scales. This paper describes a method of assessing the suitability of simple models for simulating non-linear undrained soil stress-strain behaviour in the moderate strain range. The moderate strain range is defined by a soil strength mobilisation of 20% to 80%. Three simple stress-strain models are compared. A published database of reconstituted triaxial tests is used to evaluate the three models with selected statistical tools that quantify errors associated with the simple model approximation of the relationship between stress and strain. The paper discusses the value of computing the model error and the trade off to make between introducing a greater number of parameters (and tests) for model precision and limiting the complexity of the variability characterisation