Prevalence of Sleep Paralysis in the Medical Students of a Local Medical College

Abstract Objective: To find out the prevalence of sleep paralysis among the medical students of a local Pakistani medical college. Methodology: This cross-sectional study was conducted on 107 medical students from, Karachi Institute of Medical Sciences (KIMS), Karachi, Pakistan. Non-probability Convenient Sampling Technique was utilized for this study. Students suffering from any psychiatric illness were excluded from this study. The study was performed after taking permission from the Institutional Ethical Review Committee. Written informed consent was taken from all the students. The data were collected using a pretested predesigned Questionnaire which was consisted of 10 questions related to the common10 happening and symptoms of sleep paralysis. Data were recorded in the form of frequency and percentages and analyzed using SPSS 24. Results: 52% (56 out of 107) participants have had an experience of sleep paralysis. Most of the participants (76.6%) described their experience as an Inability to move. Frequency of experience ranged from 0 to 20 times in the last year. Age at first experience ranged between 10-20 years. Conclusion: We found a high prevalence of sleep paralysis (47.2%) among the medical students at a local Pakistani medical college. Majority of the students experienced the phenomenon multiple times in their life. The students associated the symptoms the factors such as academic stress, anxiety, workload, emotional experiences, sleep deprivation, work routine, sleep pattern, sleep deprivation, fatigue, mental disturbance, studies and routine stress. 37.3% of the students also related the phenomenon to their position of sleep (lying on back). The participants described the experience as a source of great distress. Sleep paralysis needs to be studied, investigated and addressed in context of mental health of the students

Tolerance of Roselle (Hibiscus Sabdariffa L.) Genotypes to Drought Stress at Vegetative Stage

Background: Hibiscus sabdariffa L. is an important medicinal and fiber plant in Sudan. Among other stresses, drought extremely limits the growth, quality and net yield of the crop. The drought effects the crop plants by imposing certain morphological, physiological and biochemical changes at different periods of growth.Methods: Current study was carried out in greenhouse settings at Center of Excellence in Molecular Biology (CEMB) to investigate the effects of drought stress. Five (5) different genotypes of Hibiscus Sabdariffa L., namely Baladimostadir (H1), Um shiak (H2), Abu shankal (H3), Rahad mix (H4) and Abu Najma (H5) were studied. Thirty (30) days old Roselle seedlings were drought stressed for 10 days and its implications on plant growth, gas exchange, water relation, chlorophyll content and proline accumulation were estimated. Substantial genotypic differences in their adaptive response to drought were observed.Results: Drought stress significantly affected the plant height; lowered the relative gas exchange efficiency and altered the physiological and biochemical responses.  In comparison with others, H2 and H4 genotypes tolerated the osmotic stress well with lower osmotic potential and higher osmotic adjustment, better water content, higher stomatal conductance, photosynthetic efficiency and chlorophyll content. Accumulation of osmoprotectant and gas exchange indicators clearly distinguished the responses of different genotypes towards water stress.Conclusion: Our results can be used for evaluation, screening, and manipulations of Hibiscus Sabdariffa L. genotypes for improvement of drought tolerance through conventional breeding or drought responsive gene isolation

Identification and Characterization of the Diverse Stress-Responsive R2R3-RMYB Transcription Factor from Hibiscus sabdariffa L.

Various regulatory proteins play a fundamental role to manage the healthy plant growth under stress conditions. Differential display reverse transcriptase PCR and random amplification of cDNA ends (RACE) was used to explore the osmotic stress-responsive transcripts. We identified and characterized the salt stress-responsive R2R3 type RMYB transcription factor from Hibiscus sabdariffa which has an open reading frame of 690 bp, encoding 229 long chain amino acids. In silico analysis confirmed the conserved R2 and R3 domain as well as an NLS-1 localization site. The deduced amino acids of RMYB shared 83, 81, 80, 79, 72, 71, and 66% homology with Arabidopsis thaliana, Glycine max, Oryza sativa, Zea maize, Malus domestica, Populus tremula × Populus alba, and Medicago sativa specific MYB family, respectively. We observed the gene upregulation in stem, leaf, and root tissue in response to abiotic stress. Furthermore, RMYB gene was cloned into plant expression vector under CaMV35S promoter and transformed to Gossypium hirsutum: a local cotton cultivar. Overexpression of RMYB was observed in transgenic plants under abiotic stresses which further suggests its regulatory role in response to stressful conditions. The RMYB transcription factor-overexpressing in transgenic cotton plants may be used as potential agent for the development of stress tolerant crop cultivars

Gene expression in response to Cotton Leaf Curl Virus infection in Gossypium hirsutum under variable environmental conditions

Cotton Leaf Curl Disease (CLCuD) is one of the threatening constrains of cotton production in Pakistan for which no adequate remedy is available until now. Local variety of Gossypium hirsutum (FH-142) was grown in field and infected naturally by CLCuV under variable range of temperature and humidity. Plants showed thickening of veins in lower leaf surface at 34°C and 60% relative humidity at 15days post infection (dpi) and curling of leaf margins at 33°C with 58% relative humidity at 30dpi. Remarkable leaf darkening was observed with reduced boll formation at 45dpi at 26°C and 41% relative humidity. Enation developed, severe thickening and curling of leaves intensified and plants showed dwarf growth at 60dpi at 24°C with 52% relative humidity. PCR amplification of Rep associated gene confirmed the presence of CLCuD-associated begomovirus in the infected samples. Quantitative RT-PCR confirmed the amplification and differential expression of a number of pathogen stress responsive genes at different levels of temperature and humidity. This observation predicts that Cotton Leaf Curl Virus (CLCuV) interacts with several host genes that are upregulated to make plants susceptible or suppress other genes to overcome host defense responses