The evaluation of serum brain-derived neurotrophic factor levels in pregnant women with hyperemesis gravidarum

Objectives: The aim of this study was to investigate the relationship between levels of brain-derived neurotrophic factor (BDNF), which is considered a cause of conditions such as depression and eating disorders, and hyperemesis gravidarum (HG). Material and methods: This study was conducted as a prospective study at Ankara Ataturk Training and Research Hospital in the Department of Obstetrics and Gynecology. The study included 73 pregnant women with singleton pregnancies (32 pregnant women with HG and 41 pregnant women without hyperemesis). Serum BDNF levels were compared between the two groups. Results: The mean age of the study group was 27.3 ± 3.5 years and the body mass index (BMI) was 22.4 ± 2.7 kg/m2. There is no statistically significant difference between the study group and the control group in terms of demographic data (p > 0.05). The pregnant women with HG were found to have significantly higher serum BDNF levels compared to the control group (349.1 ± 94.6 pg/mL vs 292. 3± 86.01, p = 0.009) Conclusions: Serum BDNF levels that are low in psychiatric disorders such as depression or anxiety were found as high in pregnant women with HG

UPLC-Q-TOF/MS based untargeted metabolite and lipid analysis on premature ovarian insufficiency plasma samples

© 2021 Bentham Science Publishers.Background: Metabolomics is one of the main areas to understand cellular process at molecular level by analyzing metabolites. In recent years metabolomics has emerged as a key tool to understand molecular basis of diseases, to find diagnostic and prognostic biomarkers and develop new treatment opportunities and drug molecules. Objective: In this study, untargeted metabolite and lipid analysis were performed to identify potential biomarkers on premature ovarian insufficiency plasma samples. 43 POI subject plasma samples were compared with 32 healthy subject plasma samples. Methods: Plasma samples were pooled and extracted using chloroform:methanol:water (3:3:1 v/v/v) mixture. Agilent 6530 LC/MS Q-TOF instrument equipped with ESI source was used for analysis. A C18 column (Agilent Zorbax 1.8 µM, 50 x 2.1 mm) was used for separation of the metabolites and li-pids. XCMS, an “R software” based freeware program, was used for peak picking, grouping and compar-ing the findings. Isotopologue Parameter Optimization (IPO) software was used to optimize XCMS pa-rameters. The analytical methodology and data mining process were validated according to the literature. Results: 83 metabolite peaks and 213 lipid peaks were found to be in semi-quantitatively and statisti-cally different (fold change >1.5, p <0.05) between the POI plasma samples and control subjects. Conclusion: According to the results, two groups were successfully separated through principal component analysis. Among the peaks, phenyl alanine, decanoyl-L-carnitine, 1-palmitoyl lysophosphati-dylcholine and PC(O-16:0/2:0) were identified through auto MS/MS and matched with human metabo-lome database and proposed as plasma biomarker for POI and monitoring the patients in treatment pe-riod

Evaluation of Maternal and Fetal Stress Hormones During the Process of Birth

OBJECTIVE: This study aims to determine whether mode of delivery is associated with the endocrinestress response in mother and newborn.STUDY DESIGN: This prospective observational study was conducted with 86 women with a normalsingleton pregnancy who delivered healthy infants between 37 and 41 weeks of gestation in a tertiarycenter. Study groups included; (1) women undergoing normal vaginal delivery with epidural anesthesia,(2) women undergoing vaginal delivery with immersion in water for pain relief during labor, (3) womendelivered through elective caesarean section without labor. After delivery, thyroid stimulating hormone,cortisol, insulin, prolactin and Beta-endorphin levels were measured in maternal and umbilical cordserum and their relationships between modes of delivery were investigated.RESULTS: It was found that the concentrations of cortisol and beta-endorphin after vaginal delivery withimmersion in water group in both mothers and infants were higher than other two modes of delivery andthese differences were statistically significant. Umbilical cord concentration of cortisol was the lowest inthe caesarean section group.CONCLUSIONS: Maternal and fetal stress response was found to be associated with the mode of deliveryand labor