Germplasm bank of Pisa

The germplasm bank of Pisa is a scientific facility committed to the conservation of wild plant species and to conservation-related research programs. Stored collections include seeds of endangered species, endemic species, relic populations and habitat-specialist plants. The seed bank also conserves crop wild relatives that may be considered model plants in studies of ecology and physiology of seed desiccation tolerance, and a small collection of pollen grains. Its main goal is the conservation of rare, threatened, endemic or phytogeographically relevant plant taxa in Tuscany and more generally in the Mediterranean, including southern European mountain systems

A Giraffe in the Botanic Garden of Pisa (Tuscany, Northern Italy)

The Botanic Garden of Pisa was established in 1543 as a teaching tool and research facility. As with the vast majority of its sister institutions, it focuses on plant collections. However, for a short time in the first half of the XIX century, the Botanic Garden exhibited a living giraffe, a cow, and a calf. Due to the transient nature of the exhibition, it could have easily gone unnoticed but for the fortuitous representation of the animals in a drawing of the same period and for sparse notes archived in the libraries of Pisa University. Furthermore, a XIX-century publication on the morphological and behavioural traits of three antelopes indirectly suggests that those animals had been kept in the Botanic Garden for research purposes. This paper presents the evidence of the living animal display in the Botanic Garden of Pisa and the context in which it was collecte

Morphology, germination, and storage behaviour in seeds of Tuscan populations of Fritillaria montana (Liliaceae), a rare perennial geophyte in Italy

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Mediterranean sea cliff plants: morphological and physiological responses to environmental conditions

Aims The plants of Mediterranean sea cliff ecosystems are resistant to several environmental challenges. In the present study, six species typical of the coastal rocky cliffs have been analysed in order to evaluate their diverse morphological and physiological responses to their environment across the seasons, and to examine the strategy of the ecological group to which each species belongs. Since these species are widespread across the Mediterranean region, our aim was also to highlight their ecophysiological features in habitats where the direct influence of the sea is stronger. Methods The selected species are characteristic of the sea cliffs of Elba island (Tyrrhenian sea, Italy): the halophyte Crithmum maritimum, the semideciduous Helichrysum italicum and Lavandula stoechas, and the sclerophylls Myrtus communis, Quercus ilex and Rhamnus alaternus. Four morphological traits - canopy height, leaf area, specific leaf area and leaf dry matter content - and two physiological traits - leaf water potential (LWP) and photosynthetic efficiency (PE), measured before the dawn and at midday were analysed. Water potential was measured by a pressure chamber and photosynthetic efficiency was determined by the analysis of chlorophyll fluorescence. Plant performance was also evaluated by calculating chronic (PIchr) and dynamic photoinhibition (PIdyn). Important findings Crithmum maritimum showed high resistance to the recurrent dry periods, because of the high water storage capacity of its leaves and its PE declined markedly only in July, under the harshest climatic conditions. Semideciduous taxa utilise primarily an avoidance strategy, which aims at reducing the overall leaf surface, while sclerophylls mostly show a tolerance strategy towards the prevailing stressors, as demonstrated by LWP and PE, that are lower in the sclerophylls than in the semideciduous taxa during summer, due to osmoregulation and photoinhibition, respectively. Furthermore, variability of physiological parameters was higher in the sclerophylls than in the semideciduous taxa, because the former had to withstand wider oscillations of their LWP and PE. The sclerophyllous taxa underwent a slight loss of PE also in winter, likely owing to the combined action of low temperature and high irradiance. In Mediterranean sea cliff ecosystems, the stressful combination of high irradiance, high temperatures and low rainfall typical of the summer season may have been intensified by the shallow soil which displays a poor water storage capacity. On the other hand, winter stress, caused by high solar radiation and low temperatures, does not seem to seriously affect the performance of the studied species

Marine litter and psammophytes: a case study in the Migliarino-San Rossore-Massaciuccoli Regional Park coastal sand dunes

Coastal sand dunes are one of the most impacted ecosystems in the world (1). They host various habitats of Community interest under the Habitats Directive 92/43/EEC but are profoundly affected by pollution and waste management, even within protected areas. The factors that define the amount, type and distribution of beach litter are complex and relate more to human intervention and natural variables. This work aims to analyse the composition, abundance and distribution of marine litter within a protected area in two different chronological windows, i.e. before and after the bathing season. We also attempted to verify whether the presence of waste could alter the coverage of the psammophilous vegetation. The protected area examined in this project is the Migliarino-San Rossore-Massaciuccoli Regional Park (Tuscany, Italy), where we selected three dune sites stretching along the coastline with a North/South gradient: Lecciona (2 Km2), Bufalina (1 Km2) and Calambrone (1 Km2), respectively. We adopted a stratified random sampling design, using permanent multiscale squared-plots of 16 m2, with two nested plots placed at a fixed corner of 4 m2 and 1 m2, respectively. Each site was divided into same-area strata with a spatially optimised algorithm. Inside each layer two points were randomly selected, corresponding to the field plots; overall, a total of 22 plots were sampled. Sampling took place in two sessions, one in May and the other one in November. The data collected were the total percentage coverage of litter and the individual coverage of each type of litter for each plot. Classification of marine litter followed the directives of the "Master List of Categories of Litter Items", a list drawn up by the Joint Research Centre (JRC) of the European Commission based on several classification protocols (2). At the same time, we censused the plant species in each plot and measured their total percentage coverage at each investigated spatial scale. We compiled a litter × plot matrix with the percentage of coverage of each type of waste found in the plots at the three spatial scales and in the two periods. The PERMANOVA analysis of the matrix with 4 factors (month, site, layer and plot area) highlighted that the interaction term month × site explained significantly (P< 0.001) the variance in the composition of waste at the plot level. NMDS analysis (non-metric multidimensional scaling) showed that the categories most significantly related to the variability between plots were the following: "plastic fragments less than 2.5 cm (G78)" and "plastic fragments between 2.5 and 50 cm (G79)". These two types of litter showed contrasting patterns over time: G78 increased from June to November, G79 decreased in the same period. Multivariate analyses were carried out using the software PRIMER v.7 (3) and PERMANOVA+ (4). The diachronic study of the relationship between area and the number of litter categories, carried out using the Arrhenius power law equation (5), showed that in Lecciona and Bufalina there was in November a greater growth in types of litter as the area increased in respect to the first sampling period, while in Calambrone there were no differences. According to Pearson correlation test, temporal turnover of litter categories, quantified partitioning β-diversity following (6), and the total percentage coverage of vegetation resulted inversely correlated (cor = - 0.44, P = 0.038). In conclusion, artificial polymer materials were found to be the dominant waste type (85%) at the three study sites examined, two of which were characterised by an increase in waste after the bathing season. In the third site, however, probably due to regular manual cleaning actions, no differences were found. Where vegetation is more abundant, the total number of litter categories tends to be more stable across the seasons, suggesting that psammophytes are a relevant biotic component to be considered in the analysis of spatio-temporal dynamics of coastal litter. 1) Ciccarelli D., 2014. Environ. Manag. 54, 194–204 2) Galgani F., Hanke G., Werner S., et al. (2013) JRC Scientific and Policy reports 3) Clarke K., Warwick R. (2001). Change in marine communities: an approach to statistical analysis and interpretation Ed. 2. PRIMER-E, Plymouth 4) Anderson M.J., Gorley R.N., Clarke R.K. (2008). PERMANOVA+ for PRIMER: Guide to software and statistical methods. PRIMER-E, Plymouth 5) Arrhenius O. (1921) J Ecol, 9, 95-99 6) Baselga A. (2010). Glob Ecol Biogeogr, 19, 134-14

Survival in different habitats: extreme ultramafic and calcareous soils influence on Stachys recta essential oils composition

Ultramafic soils contain large amounts of magnesium and iron; they are also frequently rich in chromium, cobalt and nickel. They represent a difficult environment for plants to grow on, as they often contain toxic amounts of magnesium and nickel, whilst lacking mineral nutrients (like silicon, phosphorus, potassium and calcium); their dark colour attracts sunlight, which leads to higher temperature and drought conditions (1). On these soils, edaphic adaptation of plants is strongly evident: plants adapted to ultramafic soils exhibit morphological traits that often differ from correlated species growing on different substrates. They are often significantly smaller and show xeromorphic foliage, with reduced leaf size as the most apparent character (2). The aim of this study was to assess the influence of edaphic adaptation to ultramafic soils on a non- morphological level, investigating its role in the composition of the essential oil produced by plants growing on these kind of soils. We investigated the composition of the essential oil (EO) of Stachys recta L. subsp. subcrenata (Vis.) Briq. collected between Nibbiaia and Gabbro (province of Livorno, Italy), grown on an ultramafic soil mainly deriving from the alteration of serpentinites. We compared the yield and the composition of this EO to the one we hydrodistilled from Stachys recta L. subsp. recta L., which had been collected in the same phenological state in La Gruzza, near Montemarcello (province of La Spezia, Italy), on calcareous substrate. Whilst the yields of the two species were comparable, the composition profiles were very different: the EO extracted from S. recta subsp. recta was mainly rich in terpene compounds, which accounted for 93.8%; the species grown on the ultramafic soils, instead, had a volatile profile dominated by non-terpene derivatives, which accounted for 55.7%. In the species grown on the calcareous soil, the main constituents were germacrene D (18.8%), b-caryophyllene (17.7%), 1,8-cineole (15.9%) and a-pinene (14.2%). The EO from S. recta subsp. subcrenata was mainly dominated by 1-octen-3-ol (38.2%), a-cadinol (6.1%), (E)-3- hexen-1-ol (5.9%) and cadinene (5.6%). Considering the extent of the differences shown by the two EOs, it is reasonable to assume that the ultramafic soil plays a central role in the plants’ secondary metabolism, leading to a very different product

Heterotopy remastered with a quantitative tool: The case study of European beech (Fagus sylvatica L. subsp. sylvatica) in peninsular Italy and Sicily

The term “heterotopic” (from the Greek roots "hetero-" meaning "other" + "topos" meaning "place" = other place) was used for the first time in biology by Haeckel (1) to define a change in germ-layer origin of reproductive organs in animals. Later, it was applied to phytogeography by Jackson (2), referring to those plant populations found on soils apparently very different from those typically occurring across their distribution range. In Italy, Negri (3) was likely the first to use this term referring to European beech (Fagus sylvatica L. subsp. sylvatica) populations occurring at low altitude. Since then, most of the further Italian authors used the term “heterotopic” mainly referring to beech or other woody species (i.e. Ilex aquifolium L., Quercus ilex L.) populations occurring out of their common altitudinal range or, in a broader sense, growing out of their typical macroclimatic context. Indeed, as regards European beech populations in Italy, those occurring below 800 m a.s.l. have been generally considered as heterotopic (4), even though in many cases stands above 800 m a.s.l. were also termed as “heterotopic” (e.g. 5, 6). Here, we highlight the need to quantify “heterotopy” and propose a standard method to test a reliable applicability of this concept. As model species, we selected the European beech in peninsular Italy and Sicily, primarily because this species is typically dominant in mountain woods in all the considered area and, historically, a number of stands have been reported as heterotopic in literature. We checked 18 bibliographic references reporting 108 populations as heterotopic (sometimes not explicitly, but with related terms like extra-zonal). We also randomly generated 305 points falling within the polygons of natural potential vegetation (7) with F. sylvatica, as provided by http://www.va.minambiente.it/, and considered them as controls. Both controls and putatively heterotopic populations were georeferenced by means of a GIS software. Climatic and altitudinal data associated with the occurrence sites were extracted from the Worldclim database (www.worldclim.org). We obtained a data matrix (413 beech stands × 6 environmental variables) that was subjected to a cluster analysis applying as the distance measure the Euclidean Distance and as group linkage method the Group Average (UPGMA), following the methodology commonly used in vegetation studies. Two main clusters were identified, with a dissimilarity index of 0.35. The first cluster is composed by all those populations (including some control points) located at an altitude ≤ 600 m a.s.l., whereas the second one includes all the remaining points. The former cluster is also characterized by those populations growing on stands with a higher mean annual temperature than the latter (t-test, difference between means = 4.09 °C, p < 0.01). These results led us to define as “quantitatively heterotopic” in Italy those beech populations located at an altitude ≤ 600 m a.s.l., and with a mean annual temperature generally higher than 12 °C. They mainly occur on the Tyrrhenian side, namely in Tuscany and Lazio, marked by a high oceanicity. Many of these populations are located outside of vegetation series with European beech and, among these, some are distant more than 20 km, so that they could be interpreted not only as heterotopic, but also as biogeographical-ecological relicts (8). Our methodology could be applied to other species in order to quantify the level of heterotopy by defining ad hoc thresholds (if any), resulting from the multivariate analysis

Crop Wild Relative endemiche in Italia: una risorsa da conservare

Il Convegno di Cagliari, di cui si presentano gli abstract, non è solo l’occasione per celebrare i dieci anni di RIBES, ma è soprattutto un incontro su cinque temi attorno ai quali ruota il lavoro quotidiano di centinaia di ricercatori, tecnici e volontari diffusi sul territorio italiano: la conservazione ex situ di specie minacciate della flora italiana, il ruolo delle banche del germoplasma negli interventi di rinaturalizzazione, la cooperazione internazionale, le ricerche innovative, i parenti selvatici delle colture (CWR). I primi due temi rientrano tra le finalità statutarie fondamentali di RIBES e sono certamente più consolidati e ricchi di esperienze comuni a tutti i nodi della rete; le esperienze internazionali testimoniano il contesto entro cui operano alcune seed-bank, in particolare quelle in aree di confine e quelle con indirizzi dedicati a particolari categorie di piante. Infine il tema conclusivo sui Crop Wild Relatives rappresenta la linea di lavoro più recente e in maggiore espansione, di grande interesse applicativo, anche in considerazione dell’applicazione del protocollo di Nagoya che regola l’accesso e la condivisione delle risorse genetiche in ambito internazionale. Il Convegno e questa raccolta evidenziano l’impegno decennale dell’associazione nella conservazione della biodiversità e al tempo stesso rappresentano una tappa per rinnovare e intensificare tale impegno nella speranza di un più ampio e significativo riconoscimento da parte delle istituzioni

Contributi per una flora vascolare di Toscana. IV (181-246)

New localities and/or confirmations concerning 66 specific and subspecific plant taxa of Tuscan vascular flora, belonging to 55 genera and 33 families are presented: Acanthus (Acanthaceae), Chenopodium (Amaranthaceae), Oenanthe, Pteroselinum (Apiaceae), Melomphis (Asparagaceae), Anthemis , Artemisia, Carduus (Asteraceae), Myosotis (Boraginaceae), Alyssum, Lepidium, Malcolmia (Brassicaceae), Phyteuma (Campanulaceae), Centranthus, Lonicera, Valerianella (Caprifoliaceae), Cerastium, Sagina, Silene (Caryophyllaceae), Helianthemum (Cistaceae), Carex, Cyperus, Rhynchospora, Schoenoplectus (Cyperaceae), Chamaesyce (Euphorbiaceae), Astragalus, Vicia (Fabaceae), Hypericum (Hypericaceae), Freesia, Iris, Romulea (Iridaceae), Stachys (Lamiaceae), Gagea (Liliaceae), Montia (Montiaceae), Ludwigia (Onagraceae), Anacamptis, Barlia, Corallorhiza (Orchidaceae), Orobanche, Rhinanthus (Orobanchaceae), Oxalis (Oxalidaceae), Linaria, Veronica (Plantaginaceae), Holcus, Phleum, Rostraria (Poaceae), Rumex (Polygonaceae), Lysimachia (Primulaceae), Ranunculus (Ranunculaceae), Alchemilla, Rosa (Rosaceae), Ruppia (Ruppiaceae), Solanum (Solanaceae), Urtica (Urticaceae), Asphodeline (Xanthorrhoeaceae). In the end, the conservation status of the units and eventual protection of the cited biotopes are discussed