21 research outputs found
Identification of clinically important chromosomal aberrations in acute myeloid leukemia by array-based comparative genomic hybridization
Complex Patterns of Altered MicroRNA Expression during the Adenoma-Adenocarcinoma Sequence for Microsatellite-Stable Colorectal Cancer
Clinical molecular testing for ASXL1 c.1934dupG p.Gly646fs mutation in hematologic neoplasms in the NGS era.
ASXL1 (additional sex combs like 1) is a gene that is mutated in a number of hematological neoplasms. The most common genetic alteration is c.1934dupG p.Gly646fs. Previous publications have shown that ASXL1 mutations have a negative prognostic impact in patients with MDS and AML, however, controversy exists regarding the molecular testing of ASXL1 c.1934dupG as polymerase splippage over the adjacent homopolymer could lead to a false-positive result. Here, we report the first study to systematically test different targeted next generation sequencing (NGS) approaches for this mutation in patients with hematologic neoplasms. In addition, we investigated the impact of proofreading capabilities of different DNA polymerases on ASXL1 c.1934dupG somatic mutation using conventional Sanger sequencing, another common method for ASXL1 genotyping. Our results confirm that ASXL1 c.1934dupG can be detected as a technical artifact, which can be overcome by the use of appropriate enzymes and library preparation methods. A systematic study of serial samples from 30 patients show that ASXL1 c.1934dupG is a somatic mutation in haematological neoplasms including MDS, AML, MPN and MDS/MPN and often is associated with somatic mutations of TET2, EZH2, IDH2, RUNX1, NRAS and DNMT3A. The pattern of clonal evolution suggests that this ASXL1 mutation might be an early mutational event that occurs in the principal clonal population and can serve as a clonal marker for persistent/relapsing disease
Establishment and Characterization by Gene Expression Profiling of a New Diffuse Large B-Cell Lymphoma Cell Line, EJ-1, Carrying t(14;18) and t(8;14) Translocations
Versatile ion S5XL sequencer for targeted next generation sequencing of solid tumors in a clinical laboratory
<div><p>Background</p><p>Next generation sequencing based tumor tissue genotyping involves complex workflow and a relatively longer turnaround time. Semiconductor based next generation platforms varied from low throughput Ion PGM to high throughput Ion Proton and Ion S5XL sequencer. In this study, we compared Ion PGM and Ion Proton, with a new Ion S5XL NGS system for workflow scalability, analytical sensitivity and specificity, turnaround time and sequencing performance in a clinical laboratory.</p><p>Methods</p><p>Eighteen solid tumor samples positive for various mutations as detected previously by Ion PGM and Ion Proton were selected for study. Libraries were prepared using DNA (range10-40ng) from micro-dissected formalin-fixed, paraffin-embedded (FFPE) specimens using the Ion Ampliseq Library Kit 2.0 for comprehensive cancer (CCP), oncomine comprehensive cancer (OCP) and cancer hotspot panel v2 (CHPv2) panel as per manufacturer’s instructions. The CHPv2 were sequenced using Ion PGM whereas CCP and OCP were sequenced using Ion Proton respectively. All the three libraries were further sequenced individually (S540) or multiplexed (S530) using Ion S5XL. For S5XL, Ion chef was used to automate template preparation, enrichment of ion spheres and chip loading. Data analysis was performed using Torrent Suite 4.6 software on board S5XL and Ion Reporter. A limit of detection and reproducibility studies was performed using serially diluted DLD1 cell line.</p><p>Results</p><p>A total of 241 variant calls (235 single nucleotide variants and 6 indels) expected in the studied cohort were successfully detected by S5XL with 100% and 97% concordance with Ion PGM and Proton, respectively. Sequencing run time was reduced from 4.5 to 2.5 hours with output range of 3–5 GB (S530) and 8–9.3Gb (S540). Data analysis time for the Ion S5XL is faster 1 h (S520), 2.5 h (S530) and 5 h (S540) chip, respectively as compared to the Ion PGM (3.5–5 h) and Ion Proton (8h). A limit detection of 5% allelic frequency was established along with high inter-run reproducibility.</p><p>Conclusion</p><p>Ion S5XL system simplified workflow in a clinical laboratory, was feasible for running smaller and larger panels on the same instrument, had a shorter turnaround time, and showed good concordance for variant calls with similar sensitivity and reproducibility as the Ion PGM and Proton.</p></div
Variant call comparison between Ion Proton and Ion S5XL.
<p>(A) Venn diagram showed variant call comparison between Ion Proton and Ion S5Xl Ion S5XL showed 97% concordance for mutation detection with Ion Proton. Eight variant calls were missed by Ion S5XL due to low coverage in the variant region of the CCP panel compared to Ion Proton (B) Correlation between Variant allelic Fraction (VAF) of mutations detected on protons and Ion S5 XL (R<sup>2</sup> = 0.70; p<0.0001) and each point on the graph represents a single variant analyzed by the Ion Proton and S5XL platforms.</p
Limit of detection study performed on Ion S5 XL platform using S530 chip.
<p>Sensitivity study using DLD1 cell line serially diluted in wild type HL60 cell line DNA at 50, 25, 12.5, 6.25, 3.12, 1.50, and 0.75%. DLD1 cell line harbors heterozygous mutations in six different genes: <i>PIK3CA</i>, <i>KRAS</i>, <i>KIT</i>, <i>TP53</i>, <i>FGFR1</i> and <i>SMO</i>.</p
Inter-reproducibility studies performed on Ion S5 XL platform using S530 chip.
<p>Reproducibility studies using one normal tumor paired CCP libraries and one OCP libraries across two different sequencing runs on S5XL platform.</p
Concordance of Ion S5XL with Ion PGM and Ion Proton for variants detected using different Ampliseq panels (CCP; OCP and CHPv2).
<p>Concordance of Ion S5XL with Ion PGM and Ion Proton for variants detected using different Ampliseq panels (CCP; OCP and CHPv2).</p