Molecular and functional characterization of gap junctions in the avian inner ear.

To analyze the fundamental role of gap junctions in the vertebrate inner ear, we examined molecular and functional characteristics of gap junctional communication (GJC) in the auditory and vestibular system of the chicken. By screening inner ear tissues for connexin isoforms using degenerate reverse transcription-PCR, we identified, in addition to chicken Cx43 (cCx43) and the inner-ear-specific cCx30, an as yet uncharacterized connexin predicted to be the ortholog of the mammalian Cx26. In situ hybridization indicated that cCx30 and cCx26 transcripts were both widely expressed in the cochlear duct and utricle in an overlapping pattern, suggesting coexpression of these isoforms similar to that in the mammalian inner ear. Immunohistochemistry demonstrated that cCx43 was present in gap junctions connecting supporting cells of the basilar papilla, in which its immunofluorescence colocalized with that of cCx30. However, cCx43 was absent from supporting cell gap junctions of the utricular macula. This variation in the molecular composition of gap junction plaques coincided with differences in the functional properties of GJC between the auditory and vestibular sensory epithelia. Fluorescence recovery after photobleaching, adapted to examine the diffusion of calcein in inner ear explants, revealed asymmetric communication pathways among supporting cells in the basilar papilla but not in the utricular macula. This study supports the hypothesis that the coexpression of Cx26/Cx30 is unique to gap junctions in the vertebrate inner ear. Furthermore, it demonstrates asymmetric GJC within the supporting cell population of the auditory sensory epithelium, which might mediate potassium cycling and/or intercellular signaling

The Deep Lens Survey Transient Search I : Short Timescale and Astrometric Variability

We report on the methodology and first results from the Deep Lens Survey transient search. We utilize image subtraction on survey data to yield all sources of optical variability down to 24th magnitude. Images are analyzed immediately after acquisition, at the telescope and in near-real time, to allow for followup in the case of time-critical events. All classes of transients are posted to the web upon detection. Our observing strategy allows sensitivity to variability over several decades in timescale. The DLS is the first survey to classify and report all types of photometric and astrometric variability detected, including solar system objects, variable stars, supernovae, and short timescale phenomena. Three unusual optical transient events were detected, flaring on thousand-second timescales. All three events were seen in the B passband, suggesting blue color indices for the phenomena. One event (OT 20020115) is determined to be from a flaring Galactic dwarf star of spectral type dM4. From the remaining two events, we find an overall rate of \eta = 1.4 events deg-2 day-1 on thousand-second timescales, with a 95% confidence limit of \eta < 4.3. One of these events (OT 20010326) originated from a compact precursor in the field of galaxy cluster Abell 1836, and its nature is uncertain. For the second (OT 20030305) we find strong evidence for an extended extragalactic host. A dearth of such events in the R passband yields an upper 95% confidence limit on short timescale astronomical variability between 19.5 < R < 23.4 of \eta_R < 5.2. We report also on our ensemble of astrometrically variable objects, as well as an example of photometric variability with an undetected precursor.Comment: 24 pages, 12 figures, 3 tables. Accepted for publication in ApJ. Variability data available at http://dls.bell-labs.com/transients.htm

Randomized Algorithms for the Loop Cutset Problem

We show how to find a minimum weight loop cutset in a Bayesian network with high probability. Finding such a loop cutset is the first step in the method of conditioning for inference. Our randomized algorithm for finding a loop cutset outputs a minimum loop cutset after O(c 6^k kn) steps with probability at least 1 - (1 - 1/(6^k))^c6^k, where c > 1 is a constant specified by the user, k is the minimal size of a minimum weight loop cutset, and n is the number of vertices. We also show empirically that a variant of this algorithm often finds a loop cutset that is closer to the minimum weight loop cutset than the ones found by the best deterministic algorithms known

Massive non-thermal radio emitters: new data and their modelling

During recent years some non-thermal radio emitting OB stars have been discovered to be binary, or multiple systems. The non-thermal emission is due to synchrotron radiation that is emitted by electrons accelerated up to high energies. The electron acceleration occurs at the strong shocks created by the collision of radiatively-driven winds. Here we summarize the available radio data and more recent observations for the binary Cyg OB2 No. 9. We also show a new emission model which is being developed to compare the theoretical total radio flux and the spectral index with the observed radio light curves. This comparison will be useful in order to solve fundamental questions, such as the determination of the stellar mass loss rates, which are perturbed by clumping.Comment: 3 pages, 1 figure, poster at Four Decades of Research on Massive Stars-A Scientific Meeting in Honour of Anthony F.J.Moffa

Determining the Nature of Late Gunn-Peterson Troughs with Galaxy Surveys

Recent observations have discovered long (up to ~110 Mpc/h), opaque Gunn-Peterson troughs in the z ~ 5.5 Lyman-alpha forest, which are challenging to explain with conventional models of the post-reionization intergalactic medium. Here we demonstrate that observations of the galaxy populations in the vicinity of the deepest troughs can distinguish two competing models for these features: deep voids where the ionizing background is weak due to fluctuations in the mean free path of ionizing photons would show a deficit of galaxies, while residual temperature variations from extended, inhomogeneous reionization would show an overdensity of galaxies. We use large (~550 Mpc/h) semi-numerical simulations of these competing explanations to predict the galaxy populations in the largest of the known troughs at z ~ 5.7. We quantify the strong correlation of Lyman-alpha effective optical depth and galaxy surface density in both models and estimate the degree to which realistic surveys can measure such a correlation. While a spectroscopic galaxy survey is ideal, we also show that a relatively inexpensive narrowband survey of Lyman-alpha-emitting galaxies is ~90% likely to distinguish between the competing models.Comment: 12 pages, 16 figures. Submitted to Ap

Stellar Population Effects on the Inferred Photon Density at Reionization

The relationship between stellar populations and the ionizing flux with which they irradiate their surroundings has profound implications for the evolution of the intergalactic medium. We quantify the ionizing flux arising from synthetic stellar populations which incorporate the evolution of interacting binary stars. We determine that these show ionizing flux boosted by 60 per cent at 0.05 < Z < 0.3 Z_sun and a more modest 10-20 per cent at near-Solar metallicities relative to star-forming populations in which stars evolve in isolation. The relation of ionizing flux to observables such as 1500A continuum and ultraviolet spectral slope is sensitive to attributes of the stellar population including age, star formation history and initial mass function. For a galaxy forming 1 M_sun yr^{-1}, observed at > 100 Myr after the onset of star formation, we predict a production rate of photons capable of ionizing hydrogen, N_ion = 1.4 x 10^{53} s^{-1} at Z = Z_sun and 3.5 x 10^{53} s^{-1} at 0.1 Z_sun, assuming a Salpeter-like initial mass function. We evaluate the impact of these issues on the ionization of the intergalactic medium, finding that the known galaxy populations can maintain the ionization state of the Universe back to z ~ 9, assuming that their luminosity functions continue to M_UV = -10, and that constraints on the intergalactic medium at z ~ 2 - 5 can be satisfied with modest Lyman continuum photon escape fractions of 4 - 24 per cent depending on assumed metallicity.Comment: 17 pages, accepted by MNRAS. BPASS models can be found at http://bpass.auckland.ac.nz