Desarrollo de un sistema de etiquetado para optimizar la secuenciación de amplicones y la determinación de alelos en el MinION

La secuención nanoporo con el dispositivo MinION es una estrategia novedosa que permite el análisis de resultados en tiempo real. El MinION, al ser un equipo portable y una tecnología poco costosa, posee un amplio rango de aplicaciones. Una de estas aplicaciones es la secuenciación de amplicones y la determinación de alelos, lo cual se realizó en la presente investigación. Como modelo de estudio se empleó el gen de la S-RNasa del capulí, una especie de interés comercial en el Ecuador. La relevancia de estudiar este gen radica en que es uno de los determinantes de la autoincompatibilidad gametofítica en el capulí, por tanto, sus alelos delimitan qué cultivares son compatibles entre sí...Nanopore sequencing with the MinION device is a novel strategy that allows the real-time analysis of results. The MinION, as a portable equipment and as an inexpensive technology, has a wide range of applications. One of these applications is the sequencing of amplicons and the determination of alleles, what was done in the present investigation. As a study model, the S-RNase gene from Black Cherry was used. This species has a commercial interest in Ecuador. The relevance of studying this gene lies in the fact that it is one of the determinants of gametophytic self-incompatibility in Black Cherry; therefore, its alleles define which cultivars are compatible..

SARS-CoV-2 detection and sequencing in heart tissue associated with myocarditis and persistent arrhythmia: A case report

Background: SARS-CoV-2 uses the human cell receptor angiotensin-converting enzyme (ACE2). ACE2 is widely present in the cardiovascular system including the myocardium and the conduction system. COVID-19 patients that present severe symptoms have been reported to have complications involving myocardial injuries caused by the virus. Here we report the detection of SARS-CoV-2 by whole genome sequencing in the endocardium of a patient with severe bradycardia. Case presentation: We report a case of a 34-year-old male patient with COVID-19 tested by PCR, he started with gastrointestinal symptoms, however, he quickly deteriorated his hemodynamic state by means of myocarditis and bradycardia. After performing an endocardium biopsy, it was possible to identify the presence of SARS-CoV-2 in the heart tissue and to sequence its whole genome using the ARTIC-Network protocol and a modified tissue RNA extraction method. The patient’s outcome was improved after a permanent pacemaker was implanted. Conclusions: It was possible to identify a SARS-CoV-2 clade 20A in the endocardium of the reported patient

Upside-down but headed in the right direction:Review of the highly versatile Cassiopea xamachana system

The upside-down jellyfish Cassiopea xamachana (Scyphozoa: Rhizostomeae) has been predominantly studied to understand its interaction with the endosymbiotic dinoflagellate algae Symbiodinium. As an easily culturable and tractable cnidarian model, it is an attractive alternative to stony corals to understanding the mechanisms driving establishment and maintenance of symbiosis. Cassiopea is also unique in requiring the symbiont in order to complete its transition to the adult stage, thereby providing an excellent model to understand symbiosis-driven development and evolution. Recently, the Cassiopea research system has gained interest beyond symbiosis in fields related to embryology, climate ecology, behavior, and more. With these developments, resources including genomes, transcriptomes, and laboratory protocols are steadily increasing. This review provides an overview of the broad range of interdisciplinary research that has utilized the Cassiopea model and highlights the advantages of using the model for future research

Genomic epidemiology of SARS-CoV-2 transmission lineages in Ecuador

Characterisation of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) genetic diversity through space and time can reveal trends in virus importation and domestic circulation and permit the exploration of questions regarding the early transmission dynamics. Here, we present a detailed description of SARS-CoV-2 genomic epidemiology in Ecuador, one of the hardest hit countries during the early stages of the coronavirus-19 pandemic. We generated and analysed 160 whole genome sequences sampled from all provinces of Ecuador in 2020. Molecular clock and phylogeographic analysis of these sequences in the context of global SARS-CoV-2 diversity enable us to identify and characterise individual transmission lineages within Ecuador, explore their spatiotemporal distributions, and consider their introduction and domestic circulation. Our results reveal a pattern of multiple international importations across the country, with apparent differences between key provinces. Transmission lineages were mostly introduced before the implementation of non-pharmaceutical interventions, with differential degrees of persistence and national dissemination.ISSN:2057-157