1 research outputs found
Auxiliary Biomembranes as a Directional Delivery System To Control Biological Events in Cell-Laden Tissue-Engineering Scaffolds
Delivery of growth factors is an
indispensable part of tissue engineering.
Here, we describe a detachable membrane-based release system composed
of extracellular matrix components that can be attached to hydrogels
to achieve directional release of bioactive molecules. This way, the
release of cytokines/growth factors can be started at a desired point
of tissue maturation or directly in vivo. As a model, we develop thin
films of an interpenetrating network of double-cross-linked gelatin
and hyaluronic acid derivatives. The use of the auxiliary release
system with vascular endothelial growth factor results in extensive
sprouting by encapsulated vascular endothelial cells. The presence
of the release system with interleukin-4 results in clustering of
encapsulated macrophages with a significant decrease in M1 macrophages
(proinflammatory). This system can be used in conjunction with three-dimensional
structures as an auxiliary system to control artificial tissue maturation
and growth