10 research outputs found
The effect of 2D(2R) substitution on the agronomical traits of winter triticale in early generations of two connected crosses
The association between genomic constitution and agronomic traits was studied in F2 plants and F3:4 families of two crosses between a winter hexaploid triticale line with a 2D(2R) chromosome substitution and two hexaploid triticale cultivars carrying the complete rye genome (BBAARR). The analyses revealed that 2D(2R) substitution reduces plant height and spikelet number per spike, increases the 1,000-kernel weight, does not reduce grain shrivelling, and promotes early heading and anthesis. 2D(2R) substitution lines exhibit deeper postharvest seed dormancy, which provides resistance to preharvest sprouting. However, 2D(2R) substitution lines are not recommended for winter hexaploid triticale cultivar development purposes due to their reduced grain productivity
ΠΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π³Π΅Π½Π° GID1 Ρ Dasypyrum villosum ΠΈ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΠΠΠ-ΠΌΠ°ΡΠΊΠ΅ΡΠ° Π΄Π»Ρ Π΅Π³ΠΎ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ
Dasypyrum villosum is an annual cereal used as a donor of agronomic traits for wheat. Productivity is one of the most important traits that breeding is aimed at. It is a very complex trait, the formation of which is influenced by many different factors, both internal (the genotype of the plant) and external. The genes responsible for the gibberellin sensitivity played a large role in multiplying yields of cereal crops. Another such gene is the Gid1, which encodes a receptor for gibberellins. This article compares the DNA sequences of the Gid1 gene obtained from six Dasypyrum villosum samples. Using a sequence of wheat and rye taken from the GenBank database (NCBI), we selected primers for regions of different genomes (A, B, and D subgenomes of wheat and the R genome of rye), and carried out a polymerase chain reaction on D. villosum accessions of diverse geographical origin. The resulting PCR product was sequenced by an NGS method. Based on the assembled sequences, DNA markers have been created that make it possible to differentiate these genes of the V genome and homologous genes of wheat origin. Using monosomic addition, substitution, and translocation wheat lines, the localization of the Gid1 gene of D. villosum was established on the long arm of the first V chromosome. A phenotypic assessment of common wheat lines carrying substituted, translocated, or added D. villosum chromosomes in their karyotype was performed. Tendency of disappearance of the first chromosome of D. villosum in the lines with added chromosomes was revealed.Dasypyrum villosum (VV) - ΠΎΠ΄Π½ΠΎΠ»Π΅ΡΠ½ΠΈΠΉ Π·Π»Π°ΠΊ, Π·Π°ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄ΠΎΠ²Π°Π²ΡΠΈΠΉ ΡΠ΅Π±Ρ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π΄ΠΎΠ½ΠΎΡΠ° Ρ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎ-ΡΠ΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π΄Π»Ρ ΠΏΡΠ΅Π½ΠΈΡΡ. ΠΠ΄ΠΈΠ½ ΠΈΠ· Π²Π°ΠΆΠ½Π΅ΠΉΡΠΈΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ, Π½Π° ΠΊΠΎΡΠΎΡΡΠΉ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π° ΡΠ΅Π»Π΅ΠΊΡΠΈΡ,- ΡΡΠΎΠΆΠ°ΠΉΠ½ΠΎΡΡΡ, ΡΠ²Π»ΡΡΡΠ°ΡΡΡ ΡΠ»ΠΎΠΆΠ½ΡΠΌ, ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΡΠΌ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠΌ. ΠΠ° Π΅Π³ΠΎ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π²Π»ΠΈΡΠ΅Ρ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²ΠΎ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ². ΠΠΎΠ»ΡΡΡΡ ΡΠΎΠ»Ρ Π² ΡΠΎΡΡΠ΅ ΡΡΠΎΠΆΠ°ΠΉΠ½ΠΎΡΡΠΈ Π·Π»Π°ΠΊΠΎΠ²ΡΡ
ΠΊΡΠ»ΡΡΡΡ ΡΡΠ³ΡΠ°Π»ΠΈ Π³Π΅Π½Ρ, ΡΠ΅Π³ΡΠ»ΠΈΡΡΡΡΠΈΠ΅ ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΎΡΠ²Π΅Ρ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ Π½Π° Π³ΠΈΠ±Π±Π΅ΡΠ΅Π»Π»ΠΈΠ½Ρ, ΠΎΠ΄Π½ΠΈΠΌ ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
ΡΡΠ°Π» Π³Π΅Π½ Gid1 , ΡΠ²Π»ΡΡΡΠΈΠΉΡΡ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠΌ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΎΡΠΌ ΡΡΠΈΡ
ΡΠΈΡΠΎΠ³ΠΎΡΠΌΠΎΠ½ΠΎΠ². ΠΡΠΈΠ²Π΅Π΄Π΅Π½ΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΡΠ°ΡΡΠΈΡΠ½ΡΡ
ΠΠΠ-ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ Π³Π΅Π½Π° Gid1 , ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Ρ Π΄Π²ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Dasypyrum villosum . ΠΡΠΏΠΎΠ»ΡΠ·ΡΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΏΡΠ΅Π½ΠΈΡΡ ΠΈ ΡΠΆΠΈ, Π²Π·ΡΡΡΠ΅ ΠΈΠ· Π±Π°Π·Ρ Π΄Π°Π½Π½ΡΡ
GenBank (NCBI), ΠΏΠΎΠ΄ΠΎΠ±ΡΠ°Π»ΠΈ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΡ Π½Π° ΡΡΠ°ΡΡΠΊΠΈ ΡΠ°Π·Π½ΡΡ
Π³Π΅Π½ΠΎΠΌΠΎΠ² (ΡΡΠ±Π³Π΅Π½ΠΎΠΌΡ Π, Π ΠΈ D ΠΏΡΠ΅Π½ΠΈΡΡ ΠΈ Π³Π΅Π½ΠΎΠΌ R ΡΠΆΠΈ) ΠΈ ΠΏΡΠΎΠ²Π΅Π»ΠΈ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΡΡ ΡΠ΅ΠΏΠ½ΡΡ ΡΠ΅Π°ΠΊΡΠΈΡ Π½Π° ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
Π΄Π°Π·ΠΈΠΏΠΈΡΡΠΌΠ° ΠΌΠΎΡ
Π½Π°ΡΠΎΠ³ΠΎ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΉ ΠΠ¦Π -ΠΏΡΠΎΠ΄ΡΠΊΡ Π±ΡΠ» ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ NGS. ΠΠ° ΠΎΡΠ½ΠΎΠ²Π΅ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ
ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ ΡΠΎΠ·Π΄Π°Π½ ΠΠΠ-ΠΌΠ°ΡΠΊΠ΅Ρ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠΈΠΉ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²Π°ΡΡ Π΄Π°Π½Π½ΡΠ΅ Π³Π΅Π½Ρ Π³Π΅Π½ΠΎΠΌΠ° V ΠΈ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΠ΅ Π³Π΅Π½Ρ ΠΏΡΠ΅Π½ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ. Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠΎΠ½ΠΎΡΠΎΠΌΠ½ΠΎ-Π΄ΠΎΠΏΠΎΠ»Π½Π΅Π½Π½ΡΡ
, Π·Π°ΠΌΠ΅ΡΠ΅Π½Π½ΡΡ
ΠΈ ΡΡΠ°Π½ΡΠ»ΠΎΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π»ΠΈΠ½ΠΈΠΉ ΠΏΡΠ΅Π½ΠΈΡΡ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π° Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Π³Π΅Π½Π° Gid1 Π½Π° Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΠ°Ρ
Dasypyrum villosum . ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅ Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π³Π΅Π½Π° Π½Π° Π΄Π»ΠΈΠ½Π½ΠΎΠΌ ΠΏΠ»Π΅ΡΠ΅ ΠΏΠ΅ΡΠ²ΠΎΠΉ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ Π³Π΅Π½ΠΎΠΌΠ° V (1VL). ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΡΠ΅Π½ΠΎΡΠΈΠΏΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΡΠ΅Π½ΠΊΠ° Π»ΠΈΠ½ΠΈΠΉ ΠΌΡΠ³ΠΊΠΎΠΉ ΠΏΡΠ΅Π½ΠΈΡΡ, ΠΈΠΌΠ΅ΡΡΠΈΡ
Π² ΡΠ²ΠΎΠ΅ΠΌ ΠΊΠ°ΡΠΈΠΎΡΠΈΠΏΠ΅ Π·Π°ΠΌΠ΅ΡΠ΅Π½Π½ΡΠ΅, ΡΡΠ°Π½ΡΠ»ΠΎΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΈΠ»ΠΈ Π΄ΠΎΠΏΠΎΠ»Π½Π΅Π½Π½ΡΠ΅ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ Dasypyrum villosum
A method for research viscoplastic characteristics of materials using a vertical gas-gun stand
A methodology of constructing dynamic strain diagrams based on the method of direct impact on a vertical gas-gun stand has been developed and theoretically verified. The methodology has been verified by a reconstruction of a given static strain diagram when substituting the physical experiment by computer modeling of an axisymmetric problem, accounting for the wave processes in the striker-specimen-measuring rod system. The main inaccuracies in determining (reconstructing) viscoplastic characteristics of materials are shown to be determined by the degree of accuracy of experimental measurements. Based on the method of characteristics, an algorithm has been developed for calculating the displacements of the impacted end of the rod based on the results of the measurements of strains by two gauges in the vicinity of the impacted and supported ends of the rod for a multiply passing strain wave. This makes it possible to analyze the deformation process up to the moment the striker stops and to compare the computational and experimental results based on the residual size of the tested specimen
Experimental and calculated approach to the study of deformation and strength characteristics of elastoviscoplastic materials by direct impact method
It is proposed to develop experimental and calculated approach to the study of the strength characteristics of elastoviscoplastic materials in a non-uniform strain-stress state. Integral characteristics (forces, displacements and displacement speed) of the deformation process of hat-shaped specimens in tension are determined by a direct impact method, and their strain β stress states are determined by numerical solution of the axisymmetric problem. The results of experimental and theoretical study of the deformation and failure of hat-shaped specimens in the presence of stress concentrators are obtained
Experimental and calculated approach to the study of deformation and strength characteristics of elastoviscoplastic materials by direct impact method
It is proposed to develop experimental and calculated approach to the study of the strength characteristics of elastoviscoplastic materials in a non-uniform strain-stress state. Integral characteristics (forces, displacements and displacement speed) of the deformation process of hat-shaped specimens in tension are determined by a direct impact method, and their strain β stress states are determined by numerical solution of the axisymmetric problem. The results of experimental and theoretical study of the deformation and failure of hat-shaped specimens in the presence of stress concentrators are obtained