33 research outputs found

    Higher spin quaternion waves in the Klein-Gordon theory

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    Electromagnetic interactions are discussed in the context of the Klein-Gordon fermion equation. The Mott scattering amplitude is derived in leading order perturbation theory and the result of the Dirac theory is reproduced except for an overall factor of sixteen. The discrepancy is not resolved as the study points into another direction. The vertex structures involved in the scattering calculations indicate the relevance of a modified Klein-Gordon equation, which takes into account the number of polarization states of the considered quantum field. In this equation the d'Alembertian is acting on quaternion-like plane waves, which can be generalized to representations of arbitrary spin. The method provides the same relation between mass and spin that has been found previously by Majorana, Gelfand, and Yaglom in infinite spin theories

    The stringent response in Streptomyces coelicolor A3(2)

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    The stringent response was elicited in the antibiotic producer Streptomyces coelicolor A3(2) either by amino acid depletion (nutritional shiftdown) or by the addition of serine hydroxamate; both led to increased levels of ppGpp and to a reduction in transcription from the four promoters of the rrnD rRNA gene set. Analysis of untreated batch cultures revealed elevated ppGpp levels at the end of exponential growth, preceding the onset of antibiotic production. The effect of provoking the stringent response on antibiotic production in exponentially growing cultures was assessed by S1 nuclease mapping of actIII, an early gene of the actinorhodin biosynthetic cluster. Expression of actIII occurred after nutritional shiftdown, but not after treatment with serine hydroxamate. Although the need for ppGpp in triggering antibiotic production remains equivocal, ppGpp synthesis alone does not appear to be sufficient to initiate secondary metabolism in S. coelicolor A3(2).

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    The RNA polymerase-binding protein RbpA confers basal levels of rifampicin resistance on Streptomyces coelicolor

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    RbpA is an RNA polymerase-binding protein that occurs in the actinomycete family of bacteria and is regulated by the disulphide stress-response sigma factor, sigma(R), in Streptomyces coelicolor. Here we demonstrate that rbpA null mutants exhibit a slow-growth phenotype and are particularly sensitive to the transcription inhibitor rifampicin. Strikingly, transcription mapping experiments revealed that rbpA expression is induced upon exposure of S. coelicolor to rifampicin and that this, in part, involves an increase in the activity of sigma(R). In contrast, the ribosomal RNA operon promoter rrnDp3, which is recognized by the vegetative sigma factor sigma(HrdB), was strongly inhibited by rifampicin. Reconstitution of RNAP from an rbpA null mutant with purified RbpA revealed that RbpA stimulates transcription from rrnDp3, even in the presence of rifampicin. The data presented suggest that RbpA confers basal levels of rifampicin resistance and is a novel regulator of rRNA synthesis in S. coelicolor
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