1 research outputs found
Screening of Five and Six-Membered Nitrogen-Containing Heterocyclic Compounds as New Effective Stimulants of Linum Usitatissimum L. Organogenesis in Vitro
Linum usitatissimum L. (flax or linseed) is one of the oldest economically important crops used in biotechnology as a source
for bioactive compounds. Flax fiber and seed have industrial significance as a source of cellulosic fiber for textile and paper
industry and seed oil for pharmaceutical, cosmetic and food industry. The elaboration of new effective techniques for in
vitro regeneration of Linum usitatissimum L. is very actual problem today. The influence of low molecular weight five and sixmembered
nitrogen-containing heterocyclic compounds on organogenesis of Linum usitatissimum L. cultivar heavenly in vitro
has been studied. It was found that heterocyclic compounds derivatives pyridine, pyrimidine, pyrazole and isoflavones revealed
high stimulating effect on direct shoot organogenesis of Linum usitatissimum L. in vitro. The maximal shoot regeneration
frequency was obtained on the MS media containing heterocyclic compounds at the concentration 10-8 M/l as compared to
lower regeneration frequency obtained on the control MS medium containing 1 mg/l BAP and 0.05 mg/l NAA. The maximal
index of explants with regeneration (in %) that exceeded almost twice the same index in control MS medium was obtained
on the MS medium containing heterocyclic compound derivative of isoflavones. At the same time, the index of explants
with regeneration (in %) that exceeded one and a half times the same index in control MS medium and the maximal index
of shoots elongated more than 1 cm (in %) that exceeded almost twice the same index in control MS medium were obtained
on the MS media containing heterocyclic compounds derivatives of pyridine, pyrimidine and pyrazole. The current study
confirms perspective of using of low molecular weight five and six-membered nitrogen-containing heterocyclic compounds
at the concentration 10-8 М/l of the MS medium as new effective substitutes of traditional growth regulators auxin NAA and
cytokinin BAP for regeneration of Linum usitatissimum L. cultivar heavenly in vitro