The Impact of CRISPR-Cas System on Antiviral Therapy

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein nuclease (Cas) is identified as an adaptive immune system in archaea and bacteria. Type II of this system, CRISPR-Cas9, is the most versatile form that has enabled facile and efficient targeted genome editing. Viral infections have serious impacts on global health and conventional antiviral therapies have not yielded a successful solution hitherto. The CRISPR-Cas9 system represents a promising tool for eliminating viral infections. In this review, we highlight 1) the recent progress of CRISPR-Cas technology in decoding and diagnosis of viral outbreaks, 2) its applications to eliminate viral infections in both pre-integration and provirus stages, and 3) various delivery systems that are employed to introduce the platform into target cells

Developing a framework for multi channel e marketing

This paper is about combining three E-marketing tools including Search engine Optimization, Social Media Marketing and Email marketing to develop a new multiple channel E-marketing framework. A marketing framework can be generally defined as a written document that details the current marketing situation, threats and opportunities, marketing objectives, and the strategies for achieving the objectives. A marketing plan can be written for each product, service, brand, or for the company as a whole. An e-marketing plan is more focused than the traditional marketing plan. An e-marketing plan is more focused comparing to the traditional marketing plan. Although it often includes some topics that are used in traditional marketing plan too and it is more centered on the marketing opportunities, threats, objectives and strategies on the Internet. Having an effective online marketing framework is the most important part of marketing strategy for any companies. World Wide Web appropriates effective tools for marketers such as Social Media, Search engines, online advertisement, Email marketing, pop up Advertisement and etc. There are many E-marketing frameworks which support EMarketing campaign but they only use one tool or a mixes tool. Marketing expert’s focused on Search engine optimization and social media as a new emerging trend in an online environment. This study is going to make more benefits by adding email marketing to these new trends of E-marketing. This study has been done in Universiti Technologi Malaysia. Due to data collection 200 questionnaires have been distributed among the respondent and 152 has been collected, after analyzing collected data authors proposed a new framework for marketing, this framework has been evaluated by marketing experts. After evaluation authors can say that this study achieved its goal. The proposed framework named Multiple Channels Framework for E-marketing

Effects of a digital guided peer feedback system on student learning and satisfaction

This study investigates the effects of a digital guided peer feedback on students’ learning and satisfaction. Participants were 203 students who were randomly assigned to groups of three. students were asked to explore various perspectives, and the 'pros and cons' on the topic of 'Genetically Modified Organisms (GMOs)'. The findings show that the digital module fosters students’ learning and satisfaction.</p

Analysis and optimisation of selected genomic algorithms

The importance of genomic applications in the fields of medicine, agriculture, environment etc., has focused attention in the area of genomic computation in the last two decades. New technologies make it affordable to extract genomic information (sequencing) on a scale hitherto unknown. This has resulted in decreasing the price of sequencing and has increased the number of areas in which the sequencing data is utilised. Thus there is a need to assemble more and more genomes. A significant computational effort is needed to process this sequenced data (assembly) to assemble data and search for variations. It has been predicted that genomic data will exceed the amount of astronomical data in a near future. The growth in computational capacity, based on Moore’s law, cannot continue to respond to this increased computational demand.This thesis is motivated in response to the extensive demand for processing of sequenced data. The author identifies several important related processes and aims to improve each of those methods. First, a comprehensive review has been done on recent assembly pipelines to evaluate them. The result of the study reveals important facts which are used to design an efficient assembly practice. Second, a novel assembly pipeline is introduced that successfully balances the trade-off between speed and accuracy. Third, a fast and accurate sequence alignment algorithm is proposed that is the core of several steps in the assembly workflow, as well as a wide range of other related analysis. Finally, a new data normalisation method is designed. Due to the probabilistic nature of genome assembly, evaluating accuracy is critical. The normalisation is a vital part of the evaluation process. Along with normalisation method, the author has proposed a metric to measure how well the data is normalised. Such a metric has been proposed for the first time.The proposed assembly pipeline is 6 times faster than Spades and results in 100 times larger contiguity than SOAPdenovo2. The proposed alignment algorithm is 14 times faster than the Smith-Waterman algorithm. Yet, for the 99.99% of input sequence pairs, the proposed alignment algorithm results in the same alignment as the one that Smith-Waterman algorithm produces. Finally, the proposed normalisation method is 949 times more accurate than vt-Normalize

Efficient and Concurrent Reliable Realization of the Secure Cryptographic SHA-3 Algorithm

The secure hash algorithm (SHA)-3 has been selected in 2012 and will be used to provide security to any application which requires hashing, pseudo-random number generation, and integrity checking. This algorithm has been selected based on various benchmarks such as security, performance, and complexity. In this paper, in order to provide reliable architectures for this algorithm, an efficient concurrent error detection scheme for the selected SHA-3 algorithm, i.e., Keccak, is proposed. To the best of our knowledge, effective countermeasures for potential reliability issues in the hardware implementations of this algorithm have not been presented to date. In proposing the error detection approach, our aim is to have acceptable complexity and performance overheads while maintaining high error coverage. In this regard, we present a low-complexity recomputing with rotated operands-based scheme which is a step-forward toward reducing the hardware overhead of the proposed error detection approach. Moreover, we perform injection-based fault simulations and show that the error coverage of close to 100% is derived. Furthermore, we have designed the proposed scheme and through ASIC analysis, it is shown that acceptable complexity and performance overheads are reached. By utilizing the proposed high-performance concurrent error detection scheme, more reliable and robust hardware implementations for the newly-standardized SHA-3 are realized

Exercise-Based VRT Shows Benefits in Balance and Postural Stability in Adult Patients with Unilateral Vestibular Hypofunction

Background and Objective: Vestibular rehabilitation therapy (VRT) is an exercise-based program designed to improve balance function and alleviate problems related to dizziness, including vestibular hypofunction. This study aimed to evaluate the therapeutic effects of VRT exercises on balance function in adults with unilateral vestibular hypofunction (UVH). Methods: Thirty-one patients (mean age: 39.48 (±10.96) years old) with confirmed UVH participated in this study. The VRT program consisted of habituation and adaptation exercises in combination with gait and balance exercises. The handicapping influences of dizziness were measured with a dizziness handicap inventory (DHI) scale to measure the level of respondent’s performance on physical, emotional, and functional dimensions. Visual analogue scale (VAS) was also used to rate the severity of dizziness symptoms. The DHI and VAS scales were assessed pre-intervention and post-intervention at one, two, and four weeks follow-ups after the last rehabilitation session. Results: An analysis of variance showed a significant reduction and a consequent improvement in DHI scores after vestibular exercises in all domains: emotional, physical and functional (p &lt; 0.001). The mean total DHI score significantly decreased by 15 points after one week, 24 points after two weeks and 15 points after four weeks of the last VRT session. The mean baseline VAS score was 7.71 (±0.91) that significantly decreased at one, two, and four weeks post-intervention to 4.81 (±0.73), 3.77 (±0.61), and 3.16 (±0.97), respectively (p&lt;0.001). Conclusion: The exercise-based VRT shows benefits for adult patients with chronic dizziness concerning improvement in the vertigo symptom scale, fall risk, balance and emotional status