Four new species of Cystolepiota (Agaricaceae, Agaricales) from northeastern China

Cystolepiota is a tiny lepiotaceous fungi. During our 3 years fieldwork, we found four new species of Cystolepiota from northeastern China. A phylogenetic study of a combined dataset of ITS+nrLSU+rpb2+tef1-α revealed that Cystolepiota changbaishanensis and Cystolepiota hetieri are sister clades; Cystolepiota hongshiensis belongs to Cystolepiota seminuda complex; Cystolepiota luteosquamulosa formed a clade not closely related with any other; Cystolepiota nivalis and Cystolepiota sp. (HMJAU68235) formed a sister clade. All new species are provided with descriptions, photos of the basidiomata, and colored illustrations of the microstructures. A key for the identification of Cystolepiota species from China is also presented

Cortinarius jiaoheensis T. Bau & Y. Luo 2021, sp. nov.

Cortinarius jiaoheensis T. Bau & Y. Luo sp. nov. (Figs.2–4) MycoBank number:— MB838067 Diagnosis:—Basidiomata small. Pileus 6–12 mm diam, at first conical later almost plane with a prominent umbo, surface completely covered by woolly, whitish squamules. Lamellae emarginate, leather brown(6E6). Stipe slender, cylindrical, surface covered by white fibrils, greyish brown underneath, with rich white mycelium at the base. Universal veil white, forming zones on the stipe. Odor in context indistinct, not observed in lamellae. Taste somewhat spicy. Basidiospores 7.8–9.2 × 5.8–6.8 μm, broadly ellipsoid to somewhat obovoid ellipsoid, light brown (6D8) to rust brown (6E8), verrucose. Basidia with (2) 4 sterigmata. Lamellae margin with sterile cells. Etymology:—‘jiaoheensis’ refers to its occurrence in Jiaohe County, Jilin Province, China. Holotype:— CHINA. Jilin Province: Shansong ridge, Jiaohe County, Jilin City, 43°55′19″N, 127°05′17″E, elev. 483 m, Tolgor Bau, 3 September 2018 (HMJAU56920!). Description:— Basidiomata small. Pileus 6–12 mm diam, conical when young, later almost applanate to applanate with a prominent umbo, margin undulating; margin initially incurved, then slightly applanate to sometimes upturned; surface greyish brown (5D3–5E3) but completely covered by woolly, greyish to whitish squamules making the overall appearance pale, squamules flattened to slightly erect, dense at centre, becoming sparser and extending to the edge of the pileus. Lamellae moderately broad, with three lamellulae, light brown (6D6) when young, later leather brown (6E6), margin uneven, L=23–28, I=1–3. Stipe 17–26 mm long, 1–2 mm thick, central, cylindrical and hollow, equal in width, fragile, greyish brown (5E3), surface covered with white fibrils, with copious white mycelium at the base. Universal veil white, forming zones on the stipe. Context thin, pale white (4A1) to yellowish grey (4B2), unchanging upon cutting. Odor in context indistinct, not observed in lamellae. Taste slightly spicy. Basidiospores 7.8–9.2 × 5.8–6.8 μm (n=20), Q=1.23–1.50, Qav=1.37, broadly ellipsoid to somewhat obovoidellipsoid, light brown (6D8) to rust brown (6E8), verrucose. Basidia 24.3–29.2 × 9.7–11.7 μm, clavate, thin-walled, with two or four sterigmata, colorless to sometimes yellowish. Lamella margin with sterile cells, 19.5–27.3 × 7.8–9.8 μm, clavate, colorless. Pileipellis an epicutis of hyphae 6–13 μm wide, cylindrical, colorless to sometimes yellowish, some encrusted; hypoderm well developed, elements 15–25 μm wide, thin-walled, hyaline, subcellular; clamp connections present. Habitat:—Scattered in broadleaved forests near Quercus mongolica, fruiting in autumn. Known distribution:— Jilin Province, China. Additional material examined:— CHINA. Jilin Province: Shansong ridge, Jiaohe County, Jilin City, 43°55′19″N, 127°05′17″E, elev. 483 m, Tolgor Bau, 3 September 2018, HMJAU56920.Published as part of Luo, Ying & Bau, Tolgor, 2021, Cortinarius jiaoheensis (Cortinariaceae), a new species of Cortinarius subgenus Telamonia section Flexipedes, from northeast China, pp. 113-121 in Phytotaxa 494 (1) on pages 117-119, DOI: 10.11646/phytotaxa.494.1.7, http://zenodo.org/record/542335

Recognition of Mycena sect. Amparoina sect. nov. (Mycenaceae, Agaricales), including four new species and revision of the limits of sect. Sacchariferae

Phylogenetic reconstruction revealed that Mycena stirps Amparoina, which is traditionally classified in sect. Sacchariferae, should be treated at section level. Section Amparoina is characterised by the presence or absence of cherocytes, the presence of acanthocysts and spinulose caulocystidia. Eight species referred to Mycena sect. Amparoina sect. nov. are recognised in China. Of these taxa, four new species classified in the new section are formally described: M. bicystidiata sp. nov., M. griseotincta sp. nov., M. hygrophoroides sp. nov. and M. miscanthi sp. nov. The new species are characterised by the absence of both cherocytes and a basal disc, along with the presence of acanthocysts on the pileus, spinulose cheilocystidia and caulocystidia. Descriptions of the new species, accompanied by illustrations of morphological characters and comparisons with closely related taxa, are provided. A multi-locus analysis utilising the ITS + nLSU + SSU regions was carried out using maximum likelihood and Bayesian Inference. A key to the 12 species of sect. Amparoina sect. nov. and sect. Sacchariferae that are found in China is provided

Modicella guangxiensis (Mortierellomycota, Mortierellaceae), a new species from south-western karst areas of China

Modicella Kanouse (1936) is the only genus of Mortierellaceae known to produce macroscopic fruiting bodies in the form of small, whitish, round sporocarps. Specimens which belong to Modicella were collected during our field investigations in tropical karst areas of China. Based on morphological characteristics and phylogenetic analysis, a new species is introduced.Modicella guangxiensis is described as a new species from tropical karst areas of China. The main distinguishing characteristics of M. guangxiensis are the number of sporangiospores per sporangium (11–18), the size of sporangiospores (14–34 × 12–27.5 μm) and the surface of some hyphae with hemispherical tuber. The phylogenetic analyses, based on the internal transcribed spacer (ITS) and the large subunit (LSU) regions of rDNA sequences using Bayesian (BA) and Maximum Likelihood (ML) methods showed that the new taxon is closely related to M. reniformis

Recognition of Mycena sect. Amparoina sect. nov. (Mycenaceae, Agaricales), including four new species and revision of the limits of sect. Sacchariferae

Phylogenetic reconstruction revealed that Mycena stirps Amparoina, which is traditionally classified in sect. Sacchariferae, should be treated at section level. Section Amparoina is characterised by the presence or absence of cherocytes, the presence of acanthocysts and spinulose caulocystidia. Eight species referred to Mycena sect. Amparoina sect. nov. are recognised in China. Of these taxa, four new species classified in the new section are formally described: M. bicystidiata sp. nov., M. griseotincta sp. nov., M. hygrophoroides sp. nov. and M. miscanthi sp. nov. The new species are characterised by the absence of both cherocytes and a basal disc, along with the presence of acanthocysts on the pileus, spinulose cheilocystidia and caulocystidia. Descriptions of the new species, accompanied by illustrations of morphological characters and comparisons with closely related taxa, are provided. A multi-locus analysis utilising the ITS + nLSU + SSU regions was carried out using maximum likelihood and Bayesian Inference. A key to the 12 species of sect. Amparoina sect. nov. and sect. Sacchariferae that are found in China is provided

Recognition of Mycena sect. Amparoina sect. nov. (Mycenaceae, Agaricales), including four new species and revision of the limits of sect. Sacchariferae

Phylogenetic reconstruction revealed that Mycena stirps Amparoina, which is traditionally classified in sect. Sacchariferae, should be treated at section level. Section Amparoina is characterised by the presence or absence of cherocytes, the presence of acanthocysts and spinulose caulocystidia. Eight species referred to Mycena sect. Amparoina sect. nov. are recognised in China. Of these taxa, four new species classified in the new section are formally described: M. bicystidiata sp. nov., M. griseotincta sp. nov., M. hygrophoroides sp. nov. and M. miscanthi sp. nov. The new species are characterised by the absence of both cherocytes and a basal disc, along with the presence of acanthocysts on the pileus, spinulose cheilocystidia and caulocystidia. Descriptions of the new species, accompanied by illustrations of morphological characters and comparisons with closely related taxa, are provided. A multi-locus analysis utilising the ITS + nLSU + SSU regions was carried out using maximum likelihood and Bayesian Inference. A key to the 12 species of sect. Amparoina sect. nov. and sect. Sacchariferae that are found in China is provided

The Northeast Chinese species of Psathyrella (Agaricales, Psathyrellaceae)

Twenty seven species of Psathyrella have been found in Northeast China. Amongst them, P. conica, P. jilinensis, P. mycenoides and P. subsingeri are described as new species, based on studying morphological characteristics and phylogenetic analyses. Detailed morphological descriptions, line drawings and photographs of the new species are presented. Phylogenetic analysis of the nuclear ribosomal internal transcribed spacer (ITS) region and an identification key to the 27 Psathyrella species occuring in Northeast China are provided

Modicella guangxiensis (Mortierellomycota, Mortierellaceae), a new species from south-western karst areas of China

Modicella Kanouse (1936) is the only genus of Mortierellaceae known to produce macroscopic fruiting bodies in the form of small, whitish, round sporocarps. Specimens which belong to Modicella were collected during our field investigations in tropical karst areas of China. Based on morphological characteristics and phylogenetic analysis, a new species is introduced.Modicella guangxiensis is described as a new species from tropical karst areas of China. The main distinguishing characteristics of M. guangxiensis are the number of sporangiospores per sporangium (11–18), the size of sporangiospores (14–34 × 12–27.5 μm) and the surface of some hyphae with hemispherical tuber. The phylogenetic analyses, based on the internal transcribed spacer (ITS) and the large subunit (LSU) regions of rDNA sequences using Bayesian (BA) and Maximum Likelihood (ML) methods showed that the new taxon is closely related to M. reniformis

De novo assembly and characterization of the transcriptome of a wild edible mushroom Leucocalocybe mongolica and identification of SSR markers

Leucocalocybe mongolica is a prestigious rare wild edible mushroom in Northeast Asia. It is the unique species of the genus and the studying of its phynotype and genome is crucial to genus and species classification. Beyond that, it has high application and ecological value in the industry of food and atmosphere investigation. On account of the fact that transcriptomic and genomic data of L. mongolica lacked in the biological information database, that is a limitation to further study. The transcriptome data were obtained by virtue of Illumina paired-end sequencing technology: 42,622,958 clean reads were achieved and 37,302 contigs were generated. These contigs were subsequently assembled into 13,821 unigenes. These unigenes were annotated within 7 public databases. The 3914 unigenes were associated with a COG classification. Throughout all of the unigenes, 6642 were classified as three functional groups; 3110 unigenes were selected from KEGG pathways, and taken to further clustering analysis for 5 main categories; 57 genes, potentially involved in terpenoid, steroid, and unsaturated fatty acids biosynthesis were identified and selected for further research. The total number of carbohydrate-active enzymes of L. mongolica is 446 and the number of carbohydrate binding module (CBM) is relatively low. CE11, GT19, GT51, GT56, GH131, GH133, GH135 constitute the characteristic carbohydrate-active enzymes subfamily compared to other edible mushrooms. The characteristic carbohydrate enzymes relative to other mushrooms could play a vital role on the metabolism of nutrients. In these generated sequences, 1860 SSRs were identified and characterized as molecular candidate markers existing L. mongolica

Four New Species of Jelly Fungi from Northeastern China

Four new species of jelly fungi were described from northeastern China based on morphological and molecular evidence. These new species were classified into the four genera Sirobasidium (Sirobasidium jilinense), Calocera (Calocera velutina), Dacrymyces (Dacrymyces jauensis), and Dacryopinax (Dacryopinax manghanensis). Maximum likelihood and Bayesian analyses were performed using a combined nuc rDNA internal transcribed spacer region (ITS) and nuc 28S rDNA (nrLSU) dataset for the construction of phylogenetic trees. Morphological descriptions, line illustrations, and the ecological habits of these new species are provided