Spatiotemporal Analysis of Land Use / Land Cover in Swat, Pakistan Using Supervised Classification in Remote Sensing: 2000 to 2015

Changes in land use and land cover affect the social, economic and natural aspects of any area. Mostly land use and land cover (LULC) changes are the result of population growth and human activities in the form of urban agglomerations and industrialization etc. Physical factors like soil structure and type, slope condition, topography are main aspects. Land use change defines the historical pattern that how people used that specific land which depends on the availability of resources and economic conditions. LULC changes may trigger the detrimental effects like increase in natural hazard events and changes in climatic patterns. Climatic pattern directly affects the precipitation, groundwater recharge, the amount of evapotranspiration and runoff generation. On regional and local scale, LULC change is a far-reaching issue because environment and climate condition depend on i

Production of Remedial Proteins through Genetically Modified Bacteria

Recombinant DNA technology has created biological organisms with advanced genetic sequences and has been extensively used to express multiple genes for therapeutic purposes when expressed in a suitable host. Microbial systems such as prokaryotic bacteria has been successfully utilized as a heterologous systems showing high therapeutic potency for various human diseases. Bioengineered bacteria have been successfully utilized for producing therapeutic proteins, treating infectious diseases, and disease arise due to increasing resistance to antibiotics. Prominently E. colifound to be the most widely used expression system for recombinant therapeutic protein production i.e. hormones, enzymes and antibodies. Besides E. coli, non-pathogenic lactic acid bacteria has also been considered as an excellent candidate for live mucosal vaccine. Likewise, S. typhimuriumhas been deployed as attenuated type of vaccination as well as in treatment strategy of various cancers due to its ability of wide progression in tumors. The present article is a summarized view of the main achievements and current developments in the field of recombinant therapeutics using bacterial strains focusing on their usability in therapeutics and future potential. 

Uterine Artery Doppler Pulsatility Index in the First Trimester as a Forecaster of Pre-Eclampsia in Primary Gravida

Objective: To uncover a correlation between a high uterine artery pulsatility index on Doppler ultrasound and the early onset of pre-eclampsia to reduce maternal and fetal morbidity and mortality. Study Design: Quasi-experimental study. Place and Duration of Study: Department of Gynecology and Obstetrics, Combined Military Hospital, Multan Pakistan, from Jun 2020 to Dec 2021. Methodology: After Ethical Approval and informed consent, the umbilical artery pulsatility (UAPI) of the patients was measured by a transabdominal scan(TAS) between 75 and 100 days. Raised UAPI was correlated with early onset preeclampsia. Results: One hundred and sixty pregnant women were included in the study, out of which 34(21.3%) women developed preeclampsia, and 126(78.8%) were normal patients. Maternal weight (p=0.001), maternal BMI (p=0.002), gestational age and weight at delivery (p=0.001), and uterine artery doppler pulsatility index (p=0.001) had statistically significant differences with pre-eclampsia and regular patients among the target population. Conclusion: First-trimester uterine artery pulsatility index on Doppler ultrasound is a beneficial technique to recognize the women by risk status and target them to prevent pre-eclampsia

AXL modulates extracellular matrix protein expression and is essential for invasion and metastasis in endometrial cancer

The receptor tyrosine kinase AXL promotes migration, invasion, and metastasis. Here, we evaluated the role of AXL in endometrial cancer. High immunohistochemical expression of AXL was found in 76% (63/83) of advanced-stage, and 77% (82/107) of high-grade specimens and correlated with worse survival in uterine serous cancer patients. In vitro, genetic silencing of AXL inhibited migration and invasion but had no effect on proliferation of ARK1 endometrial cancer cells. AXL-deficient cells showed significantly decreased expression of phospho-AKT as well as uPA, MMP-1, MMP-2, MMP-3, and MMP-9. In a xenograft model of human uterine serous carcinoma with AXL-deficient ARK1 cells, there was significantly less tumor burden than xenografts with control ARK1 cells. Together, these findings underscore the therapeutic potentials of AXL as a candidate target for treatment of metastatic endometrial cancer

Ketosis prevents abdominal aortic aneurysm rupture through C-C chemokine receptor type 2 downregulation and enhanced extracellular matrix balance

Abdominal aortic aneurysms (AAAs) are prevalent with aging, and AAA rupture is associated with increased mortality. There is currently no effective medical therapy to prevent AAA rupture. The monocyte chemoattractant protein (MCP-1)/C-C chemokine receptor type 2 (CCR2) axis critically regulates AAA inflammation, matrix-metalloproteinase (MMP) production, and extracellular matrix (ECM) stability. We therefore hypothesized that a diet intervention that can modulate CCR2 axis may therapeutically impact AAA risk of rupture. Since ketone bodies (KBs) can trigger repair mechanisms in response to inflammation, we evaluated whether systemic ketosis in vivo could reduce CCR2 and AAA progression. Male Sprague-Dawley rats underwent surgical AAA formation using porcine pancreatic elastase and received daily β-aminopropionitrile to promote AAA rupture. Rats with AAAs received either a standard diet, ketogenic diet (KD), or exogenous KBs (EKB). Rats receiving KD and EKB reached a state of ketosis and had significant reduction in AAA expansion and incidence of rupture. Ketosis also led to significantly reduced aortic CCR2 content, improved MMP balance, and reduced ECM degradation. Consistent with these findings, we also observed that Ccr2-/- mice have significantly reduced AAA expansion and rupture. In summary, this study demonstrates that CCR2 is essential for AAA expansion, and that its modulation with ketosis can reduce AAA pathology. This provides an impetus for future clinical studies that will evaluate the impact of ketosis on human AAA disease

A Survey on Cybersecurity Challenges, Detection, and Mitigation Techniques for the Smart Grid

The world is transitioning from the conventional grid to the smart grid at a rapid pace. Innovation always comes with some flaws; such is the case with a smart grid. One of the major challenges in the smart grid is to protect it from potential cyberattacks. There are millions of sensors continuously sending and receiving data packets over the network, so managing such a gigantic network is the biggest challenge. Any cyberattack can damage the key elements, confidentiality, integrity, and availability of the smart grid. The overall smart grid network is comprised of customers accessing the network, communication network of the smart devices and sensors, and the people managing the network (decision makers); all three of these levels are vulnerable to cyberattacks. In this survey, we explore various threats and vulnerabilities that can affect the key elements of cybersecurity in the smart grid network and then present the security measures to avert those threats and vulnerabilities at three different levels. In addition to that, we suggest techniques to minimize the chances of cyberattack at all three levels

Appraisal of Spatiotemporal Variations in Snow Cover Dynamics using Geospatial Techniques: A paradigm from Gilgit-Baltistan, Pakistan

Snow and glaciers in the mountain ranges of Hindu-Kush, Karakoram, and Himalayas (HKH), are supposed to be shrinking. Consequently, serious significances occur in respect of water accessibility for inhabitants of down streams. This investigation is an effort o identify the temporal variation in snow covers of Gilgit Baltistan (GB), northern Pakistan. For the calculation of the Normalized Difference Snow Index (NDSI) and estimation of snow-covered area (SCA), MODIS images for the period of 2000 to 2020 were used. Hunza, Astore, Gilgit, Diamir, Shyok, Ghanche, Skardu and Shigar are major sub-basins lying in GB with Hunza and Skardu being the mostly glaciated areas. Annual fluctuation in snow cover within GB was 10 to 80%. In accumulation season, from December to February, snow cover was almost 80% while in melting season, from July to September this area was reduced to 65-75%. The maximum and minimum Snow-Covered Area (SCA) detected in 2009 was almost 57687.85 km2 and 12083.40 km2 respectively. These results show fluctuations in SCA within the time duration of the last two decades. Full Tex

سرائیکی کلاسیکی مرثیے کے ارکان خمسہ

Epic Usually ends at elegy. Saraiki Poetry begins from elegy. Saraiki Poetry has a long tradition in the historical incident of Karbala. In a specific sect of Muslims this incident is a part of their faith. In this point of view elegy shaped itself as a fun and craft. In this Article it is told about the Saraiki elegy's top five reciters who established the Skelton and shape of this Art.</p