Association of rs2476601 and rs1544410 with Onset of T1D in Youngsters of Lahore, Pakistan

The PTPN22 gene plays vital role in T1D onset by encoding Lymphoid-specific phosphatase (LYP) that lead to T-cell receptor-associated CsK kinase inactivation and preventing T-cell spontaneous activation by dephosphorylation. VDR gene encoded for VDR receptor is involved indirectly in prevention of T1D onset by promoting insulin production. The present study was conducted to determine the mutations on rs2476601 and rs1544410 polymorphic sites on the PTPN22 and VDR genes respectively. We genotyped 50 patients and 50 control subjects from Lahore by using sequencing and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (RFLP) techniques. It was observed that the allelic frequency of r and b were higher in patients as compared to controls and significantly associated with the onset of T1D. The genotype distribution frequencies varied significantly among patients and controls (p \u3c 0.01). The mutation on the rs2476601 polymorphic site led to the change of Isoleucine to tryptophan in patients. In conclusion, compelling evidence was found of T1D onset association with the polymorphism at RsaI on rs2476601 and BsmI on rs1544410 on PTPN22 and VDR genes

Terminalia arjuna restores the levels of alkaline phosphatase and aspartate aminotransferase of acetaminophen intoxicated mice

Background: Herbal medicines are natural and comparatively safer than conventional treatments and are well-documented for carrying little potential for harm.  Therefore, phytonutrients have occupied a central stage in the therapeutics. Terminalia arjuna, a medicinal plant, has been reported to have homeostatic, laxative, diuretic, antidiabetic, anticancer and cardiotonic actions.Methods: The current study was designed to investigate the protective role of T. arjuna leaf extract at three dose levels (100, 250, 500 mg/kg body weight) against acetaminophen (250 mg/kg body weight) induced liver damage.Results: The administration of hepatotoxin (Acetaminophen) resulted in disturbance of hematological and serological profile including alkaline phosphatase (ALP) and aspartate aminotransferase (AST) which was assayed in control and drug treated experimental models. Treatment with T.  arjuna leaf extract for 7 days restored the normal levels of markers and response was dose dependent.Conclusion: This study adds to the very limited existing literature regarding hepatoprotective effect of T. arjuna against acetaminophen toxicity. It is also important to get a step closer to development of accessible, authoritative, and independent information resources about herbal medicines and wide-ranging health disorders, which are currently lacking in Pakistan.Keywords: Hepatoprotective; Terminalia arjuna; ALP; AST   

Hepatoprotective Effect of Berberis Lycium in Αlpha-Naphthylisothiocyanate Induced Cholestatic Mouse Models

Cholestasis is characterized by the obstruction of bile flow from hepatocytes to the intestine. It results in accumulation of bile acids in the liver, which cause oxidative stress, inflammation, apoptosis, fibrosis, and cirrhosis. Till now, the treatment options against cholestasis are limited. Therefore, there is an utmost requirement to develop and evaluate the drugs with potential anti-cholestatic effects. In the current study, the drug-induced cholestasis mouse models were generated via oral administration of α-naphthylisothiocyanate (ANIT). The mice were placed into three groups of six animals each. Group I was the control group and was given saline. The cholestasis model Group II was given saline for 19 days. On 19th day (48 hours before sacrifice) they received a single dose of ANIT (75 mg/kg). Group III served as the plant extract treatment group and received root extract for 19 days. On nineteenth day (48 hours before sacrifice) they received a single dose of ANIT (75 mg/kg). On 21st day, mice were sacrificed for analysis of serum biochemistry and liver histology. The results revealed that Berberis lycium extract has hepatoprotective properties, as serum level of AST (aspartate aminotransferase) and ALT (alanine transaminase) are significantly lower in the plant treatment group compared to the cholestasis model group. Furthermore, liver histology validated the serological results since the hepatocyte architecture in the plant treatment group was similar to that of control group. In conclusion, the data exhibit that B. lycium possess protective activities against ANIT induced cholestasis

The Physico-Chemical Environment and Aquatic Biodiversity of Head Marala Wetland During 2000-2001

An ecological study on Head Marala Wetland was undertaken. The study will help in providing sound basis for the future conservation activities and its management, on sounder footing. The Marala wetlands in Bajwat area included river Jammu Tawi, river Chanab and river Manawar Tawi were studied for a period of one year, i.e. from October, 2000 to September, 2001. In this study, 36 Phytoplanktons belonging to six families were observed. The other aquatic fauna observed included 14 species of fish, 2 turtles, one leech, one prawn, one shrimp and one bullfrog. The results showed that physico-chemical parameters had an impact on the biodiversity and population size of the aquatic fauna at Head Marala wetlands

Shrews from Moist Temperate Forests of Azad Jammu and Kashmir

The soricidae family comprises of nearly 23 genera out of which approximately 200 species have been associated with the Eurasian region (IUCN, 1995). A lot of ambiguity surrounds this family, especially where taxonomic classification is concerned; also, not much is known about their geographic location. Five protected parks from Azad Jammu and Kashmir (AJK) were explored for population trend studies of family soricidae. Dhirkot Nature Reserve (DNR) was observed in February, 2008, Banjosa Nature Reserve (BNR) in May-June, 2009, Tolipir National Park (TNP) in April to May, 2008, Pir Chanasi National Park (PCNP) in April to May, 2010 and Pir Lasura National Park (PLNP) from June to July, 2009. Only four species belonging to the soricidae family were found; Suncus etruscus, Suncus murinus, Sorex minutus thibetanus and Crocidura pullata. The Asiatic white toothed shrew was only observed in PCNP, DNR and BNR. Suncus murinus was only observed in PCNP. Sorex minutus thibetanus was only observed in Banjosa Nature Reserve. Suncus etruscus was only observed from DNR and BNR

Checklist of Zooplanktons in Different Rivers of Bajwat Area

Zooplanktons are important fish and waterfowl food. Limnological studies of Marala Wetlands, a complex created by three rivers, i.e., Jammu Tawi, Chenab and Manawar Tawi, entering Bajwat area located 25 km from North of Sialkot city from state of Jammu and Kashmir, were carried out between October, 2000 to September 2001. This survey was carried out to create a checklist of zooplanktons existing in the study site which would help in future studies relating to aquatic biodiversity and ecological studies of wetlands. A minimum of 25 species of zooplanktons were present in the wetland area, which can be included into 4 major animal groups. Amoeba, Paramecium, Opalina and Euglena were the dominant species. Crustaceans were represented by Daphnia, Moina, Cyclops. Insects observed at river Jammu included mosquito larvae, water spider and dragonflies of unidentified species. The Maximum number of zooplanktons were recorded in March, April, May & then in September and October as observed throughout study period

Association of Interleukin 17F with Arthritis in Punjabi Families of Pakistan

Arthritis is a chronic inflammatory disease that causes severe joint pain. Interleukin 17F (IL17F) is considered as a candidate gene functionally; it mediates pro-inflammatory responses, depending on the type and site of inflammation. The present study examined the polymorphism of IL17F (rs763780 and rs2397084) among the families affected by arthritis. Demographic data and blood samples were collected from the families with at least one affected offspring with arthritis. Analysis of the IL17F gene polymorphism was performed by the digestion of DNA with NlaIII and AvaII. The results showed that IL17F rs763780 (AA, AG and GG genotypes) and rs2397084 (AA, AG and GG genotypes) were associated with arthritis (OA & RA). It was evaluated that about 65 and 21 percent of the individuals mutated with homozygous mutation for wild type allele A, heterozygous mutation A/G against selected SNPs respectively. But homozygous polymorphic allele for allele G was only found against rs2397084. Mutation in rs2397084 resulted to change Lysine into Arginine, whereas mutation in rs763780 changed Histidine into Arginine. Maternal history was found as a stronger factor in transferring arthritis. The results of this study revealed an association of arthritis with IL17F among Pakistani population

Status of Jackals and Foxes (Family: Canidae) From Selected Hill Areas of Pakistan

The family Canidae consists of coyotes, dogs, foxes, jackals, and wolves. The IUCN red list reports 38 species under this family. This study was conducted in Azad Jammu and Kashmir, Pakistan. Conserved areas of Tolipir National Park, Banjosa Game Reserve, Pir Chanasi National Park, Pir Lasura National Park and Dhirkot Nature Reserve were studied from 2008 to 2010. Vulpes vulpes griffithi (Kashmir or hill fox) is a flexible hunter. It mainly feeds on hares, rodents, reptiles and occasionally small birds. In absence of vertebrate prey, they may subsist on insects and fruit. Canis aureus (Golden Jackal) is a very adaptable animal which readily enters mountainous areas, forest plantations and riverine thickets. Only two species (Vulpes vulpes griffithi and Canis aureus) belonging to the family Canidae were found in the study area. The local sightings of C. aureus were 35% in BGR, 45% in DNR, 28% in PCNP and 40% in PLNP. Local sightings of V. vulpes species were 18% in BGR and 16% in PCNP. It was not observed in Tolipir National Park, Pir Lasura National Park and Dhirkot Nature Reserve

Small Mammals of Family Muridae in Protected Areas of Pakistan

Murids to have more than 1300 species globally, forming the largest mammal group. Murids are found nearly everywhere in the world, though many subfamilies have narrower ranges. Murids are not found in Antarctica and many oceanic islands. Five National Parks from Northern areas of Pakistan were physically surveyed. The parks were studied at different times. Dhirkot National Park (DNP) in February 2008, Banjosa National Park (BJNP) from May to June 2009, Pir Lasura National Park (PLNP) from June to July 2009 and Pir Chanasi National Park (PCNP) from April to May 2010. A total of 6 species belonging to the Muridae family were found cumulatively in the study areas