2 research outputs found
Polydiacetylene Nanovesicles as Carriers of Natural Phenylpropanoids for Creating Antimicrobial Food-Contact Surfaces
The ultimate goal of this study was
developing antimicrobial food-contact
materials based on natural phenolic compounds using nanotechnological
approaches. Among the methyl-β-cyclodextrin-encapsulated phenolics
tested, curcumin showed by far the highest activity toward <i>Escherichia coli</i> with a minimum inhibitory concentration
of 0.4 mM. Curcumin was enclosed in liposome-type polydiacetylene/phosholipid
nanovesicles supplemented with <i>N</i>-hydroxysuccinimide
and glucose. The fluorescence spectrum of the nanovesicles suggested
that curcumin was located in their bilayer region. Free-suspended
nanovesicles tended to bind to the bacterial surface and demonstrated
bactericidal activity toward Gram-negative (<i>E. coli</i>) and vegetative cells of Gram-positive (<i>Bacillus cereus</i>) bacteria reducing their counts from 5 log CFU mL<sup>–1</sup> to an undetectable level within 8 h. The nanovesicles were covalently
bound to silanized glass. Incubation of <i>E. coli</i> and <i>B. cereus</i> with nanovesicle-coated glass resulted in a 2.5
log reduction in their counts. After optimization this approach can
be used for controlling microbial growth, cross-contamination, and
biofilm formation on food-contacting surfaces