STUDY OF THE EFFECTS OF N-FERTILIZATION AND PLANT DENSITY ON THE RESISTANCE OF MAIZE HYBRIDS TO FUSARIAL EAR ROT

Eight maize hybrids grown in Hungary were studied in order to investigate the effects of some agrotechnical factors (N-fertilization and plant density) on the resistance to Fusarium moulds. The plants grown on an experimental farm were artificially infected using the tooth pick method with F. graminearum and F. culmorum. In addition, maize grain meals were also inoculated with isolates of moulds. The surface area of ears covered by moulds 9 weeks after inoculation and the toxin content (Zearalenone and T2 toxin) of infected grain meals were measured. For characterization of resistance, the toxin-mould index (TMI) was introduced which takes into consideration both the growth rate of moulds and their toxin production. A slight decrease in the mould-covered surface area of ears was observed with growing N-supply, and surprisingly no significant differences were found in the toxin content with higher plant density

A NEW METHOD FOR THE EVALUATION OF THE RESISTANCE OF MAIZE HYBRIDS TO FUSARIAL EAR ROT - THE TOXIN-MOULD-INDEX (TMI)

30 maize hybrids grown in Hungary representing the groups FAO 200 - 299, FAO 300 399, FAO 400 - 499, and FAO 500 - were studied in order to gain information about genotypic resistance to Fusarium moulds. The plants grown on an experimental farm were artificially infected using the tooth pick method with F. graminearum and F. oxysporum resp. In addition maize grain meals were also inoculated with isolates of moulds. The surface area of ears covered by moulds 9 weeks after inoculation and the toxin content (zearalenone and T-2 toxin) of infected maize grain meals were measured. Big differences were observed in the mould-covered area of the ear surface (2.00 to 38.88%) and also in the zearalenone content (2.73 to 80.33 mg per kg) and T-2 toxin content (5.37 to .52.33 mg per kg) between genotypes. Poor correlation was found between the mould-covered area and the toxin content measured in the maize grain meal of the same genotypes. For characterization of resistance resp. susceptibility of different genotypes, a new method was introduced using the toxin-mould-index (TMI) taking in to consideration both the growth rate of moulds and their toxin production. In every FAO group resistant genotypes were found confirming the view that not only the duration of the vegetation period influences the resistance or susceptibility

Cerebral iodized lipid embolization via a pulmonary arteriovenous shunt: rare complication of transcatheter arterial embolization for hepatocellular carcinoma

We report the first European case of cerebral iodized lipid embolism post transcatheter arterial embolization for hepatocellular carcinoma. Lipiodol emboli and corresponding multifocal brain ischemia were documented with computed tomography (CT) and magnetic resonance (MR) in the acutely symptomatic patient. Transcranial Doppler sonography with contrast indicated a right-to-left shunt, while on a follow-u

A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS

This study describes cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from one liter of E.coli culture. Enzymatic activity measurements of the ammonia elimination reaction from different natural aromatic amino acids proved the protein to be a phenylalanine ammonia-lyase. The isolated protein showed remarkably high, 81.7 °C melting temperature, making it especially suitable for biocatalytic applications

Essential role of using virtual pyeloscopy in the diagnosis of small satellite renal pelvic tumour in solitary kidney patient

Virtual pyeloscopy (VP) plays an important role in the organ-sparing nephroscopic tumour resection and traditional pole resection, especially when a fibreoptic ureteroscopic examination cannot be performed. A 67-year-old man developed macroscopic hematuria. Abdominal computed tomography (CT) and cystoscopy revealed a lower calix renal stone and a 20-mm lower pyelon mass suggesting transitional cell cancer. An additional small satellite lesion in the pelvico-ureteral junction was supposedly present. Three-phase MultiDetector CT with VP showed a solitary 3 to 4-mm renal pelvic lesion beyond the known calix stone and lower pole mass. In our case, VP played an important role in the organ-sparing nephroscopic tumour resection and traditional pole resection