Additional file 1: of DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer

Table S1. Primer sequences used in this study. (DOCX 18 kb

Additional file 2: of DNA methylation and repressive H3K9 and H3K27 trimethylation in the promoter regions of PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, and PD-L1 genes in human primary breast cancer

Figure S1. Methylation PCR and promoter sequences of immune checkpoints and PD-L1 genes. The upper representative blots show gel electrophoresis of the PCR products from NT or TT after bisulfite treatment using methyl primers. Lower figures show the promoter sequences with the primer details (red) and CpG sites (blue) for PD-1 (A), CTLA-4 (B), TIM-3 (C), LAG-3 (D), PD-L1 (E), and TIGIT (F). (PPTX 2479 kb

Additional file 1: of A homozygous splicing mutation in ELAC2 suggests phenotypic variability including intellectual disability with minimal cardiac involvement

UCSC genome browser. (DOCX 432 kb

Additional file 2: of Novel genetic risk variants for pediatric celiac disease

Regulome DB questioned a possible role of the variants of interest in terms of transcription factor binding sites, chromatin states, eQTLs, differentially methylated regions, validated functional SNPs and DNase sensitivity. All variants had minimum or no impact. (XLSX 3.62 mb

Additional file 1: of Novel genetic risk variants for pediatric celiac disease

A seven-member Greek family has been recruited (informed consents have been obtained), and a trio analysis (III-1, III-2, IV-3) has been performed using the celiac disease model. (PNG 136 kb