Method development and validation for the determination of cabergoline in tablets by capillary zone electrophoresis

A capillary zone electrophoresis (CZE) method has been developed for the analysis of cabergoline in its pharmaceutical preparations. Optimized analysis conditions for cabergoline analysis were performed using 110 mM pH 5.0 phosphate buffer containing 30 % acetonitrile as an electrolyte solution. Separation was performed through a fused silica capillary (50 μm i.d., total length 64.5 cm, 50.0 cm effective length) at 30 ºC with an applied voltage of 30 kV and hydrodynamic injection for 4 s. Cabergoline and internal standard verapamil were detected at a wavelength of 220 nm. The calibration was liner from 5.0 to 90.0 μg mL–1 and the limit of detection and quantification were 1.25 and 3.77 μg mL–1 Optimized CE . method was validated on the basis of related ICH guideline and found as an accurate, sensitive, precise and reproducible method for cabergoline determination. Developed method is also successfully applied for the analysis of pharmaceutical preparations containing cabergoline.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Method development and validation for the determination of cabergoline in tablets by capillary zone electrophoresis

A capillary zone electrophoresis (CZE) method has been developed for the analysis of cabergoline in its pharmaceutical preparations. Optimized analysis conditions for cabergoline analysis were performed using 110 mM pH 5.0 phosphate buffer containing 30 % acetonitrile as an electrolyte solution. Separation was performed through a fused silica capillary (50 μm i.d., total length 64.5 cm, 50.0 cm effective length) at 30 ºC with an applied voltage of 30 kV and hydrodynamic injection for 4 s. Cabergoline and internal standard verapamil were detected at a wavelength of 220 nm. The calibration was liner from 5.0 to 90.0 μg mL–1 and the limit of detection and quantification were 1.25 and 3.77 μg mL–1 Optimized CE . method was validated on the basis of related ICH guideline and found as an accurate, sensitive, precise and reproducible method for cabergoline determination. Developed method is also successfully applied for the analysis of pharmaceutical preparations containing cabergoline.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Method development and validation for the determination of cabergoline in tablets by capillary zone electrophoresis

A capillary zone electrophoresis (CZE) method has been developed for the analysis of cabergoline in its pharmaceutical preparations. Optimized analysis conditions for cabergoline analysis were performed using 110 mM pH 5.0 phosphate buffer containing 30 % acetonitrile as an electrolyte solution. Separation was performed through a fused silica capillary (50 μm i.d., total length 64.5 cm, 50.0 cm effective length) at 30 ºC with an applied voltage of 30 kV and hydrodynamic injection for 4 s. Cabergoline and internal standard verapamil were detected at a wavelength of 220 nm. The calibration was liner from 5.0 to 90.0 μg mL–1 and the limit of detection and quantification were 1.25 and 3.77 μg mL–1 Optimized CE . method was validated on the basis of related ICH guideline and found as an accurate, sensitive, precise and reproducible method for cabergoline determination. Developed method is also successfully applied for the analysis of pharmaceutical preparations containing cabergoline.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Spectrophotometric analysis of Cabergoline in pharmaceutical preparations

Cabergoline (CAB) is a synthetic ergoline dopamine agonist with high affinity to D2 receptors, thus used for the treatment of Parkinson's patients and hyperprolactinemia disorders. In this study, simple, fast, reliable and validated UV-VIS and 2nd order derivative spectrophotometric methods for determination of CAB in pharmaceutical preparations were developed without any previous sample preparation step. Determination of CAB was performed at 280 nm wavelength by UV-VIS spectrophotometry and in the range of 227-232 nm by 2nd order derivative spectrophotometry. Developed both spectrophotometric methods were linear over the range of CAB concentrations from 1 to 125 µg mL-1-. The highest relative error and relative standard deviation in within-day and day-to-day study for UV-VIS spectrophotometry were found to be 1.10% and 0.63%, respectively. The relative error and relative standard deviation in within-day and day-to-day study for 2nd order derivative spectrophotometry were not higher than 1.10% and 0.70%, respectively.Colegio de Farmacéuticos de la Provincia de Buenos Aire