Mosquito Surveillance

During 2006, mosquitoes were collected at the Western Research Farm, Castana, and McNay Research Farm, Chariton, IA in gravid traps that were baited to attract female mosquitoes that were ready to lay eggs(oviposit). The gravid trap in particular is in widespread use in mosquito control programs throughout the U.S., because the trap preferentially attracts mosquitoes that 1) are known vectors(in the genus Culex) of West Nile virus(WNV) and St. Louis Encephalitis virus (SLEV) and 2) have taken a potentially infectious blood meal (prerequisite to oviposition), thereby increasing the chance of detecting virus. Specimens were collected daily and kept cold to maintain integrity, then were mailed to campus. Once in the lab,specimens were identified to species and vector species were pooled into groups of up to 50—a standard operating procedure for surveillance labs across the U.S.—which then were subjected to virus detection assays. The results of trapping at these sites are shown according to the species of mosquitoes collected. Mosquitoes in the genus Culex (Cx. in table 1) were subjected to virus detection assays, and none of those collected at either farm tested positive for infection with WNV or SLEV

Mosquito and Arbovirus Activity

Mosquitoes are public health concerns as vectors of arthropod-borne viruses (arboviruses) and/or as nuisances to humans, so surveillance efforts are important to determine areas and times that may pose a risk to humans. The ISU Horticulture Research Station, Ames, Iowa, consistently yields mosquitoes that are positive for West Nile virus (WNV). Objectives were to continue to assess human risk by monitoring both mosquitoes and sentinel chickens, which serve as vertebrate hosts for arbovirus

Mosquito and Arbovirus Activity in 2014

Mosquitoes are public health concerns as vectors of arthropod-borne viruses (arboviruses) and/or as nuisances to humans, so surveillance efforts are important to determine areas and times that may pose a risk to humans. The ISU Horticulture Research Station (HRS), Ames, Iowa, consistently yields mosquitoes that are positive for West Nile Virus (WNV). Objectives were to continue to assess human risk by monitoring mosquitoes, which are vectors that transmit this virus to people

Evaluation of Highly Targeted dsRNA for the Treatment of Infectious Myonecrosis Virus (IMNV) in Litopenaeus vannamei

Double stranded RNA was synthesized in vitro with sequences corresponding to portions of the IMNV genome and were injected into shrimp 2 days after a lethal challenge with IMNV. This is the first time these methods have been used to treat IMNV infections in shrimp

Mosquito and Arbovirus Activity

Mosquitoes are public health concerns as vectors of arthropod-borne viruses (arboviruses) and/or as nuisances to humans, so surveillance efforts are important to determine areas and times that may pose a risk. The Horticulture Research Station (HRS), Ames, Iowa, consistently yields mosquitoes that are positive for West Nile virus (WNV). Objectives were to continue to assess human risk by monitoring both mosquitoes and sentinel chickens, which serve as vertebrate hosts for arbovirus

“Popping it” as family in Mosquitoes & Me: affective accumulation and Anzaldúan aesthetic consciousness in \u3ci\u3eCiencia Zurda\u3c/i\u3e

This article uses key concepts of Anzaldúan philosophy to describe the Mosquitoes & Me summer camp as ciencia zurda or left-handed science. It details a day-in-the-life portrait of Elena, a first-generation Latina middle schooler, as she experiences the opportunities that Mosquitoes & Me provided for self-other bridge crossing and radically relational, Nepantlan potential. Our discussion of Elena’s experiences in terms of Nepantlan principles of diversity, Affect, and spirit, as well as Affective accumulation and aesthetic consciousness, infuse an emotional and spiritual dimension to inquiry science- and responsive sciencecomplementarity and revisit a call for soul-centered, socially-transformative science. Este artículo utiliza conceptos clave de la filosofía de Anzaldúan para describir el campamento de verano Mosquitos & Me como ciencia zurda. Detalla un retrato del día a día en la vida de Elena, una estudiante de secundaria latinoamericana de primera generación, mientras experimenta las oportunidades que Mosquitoes & Me le brindó para cruzar el puente entre sí misma y el potencial Nepantla que es radicalmente relacional. Nuestra discusión sobre las experiencias de Elena en términos de los principios de Nepantla sobre diversidad, afecto y espíritu, así como la acumulación afectiva y la conciencia estética, infunden una dimensión emocional y espiritual a la complementariedad de la ciencia investigadora y la ciencia receptiva, y hacen un llamado por una ciencia centrada en el alma y socialmente transformadora

Mosquito Surveillance

As blood-sucking insects, mosquitoes are particularly important to human health. They not only are annoying pests but also are capable of transmitting debilitating or lethal pathogens, mostly viruses, to humans. In order to assess the threat posed by mosquitoes to humans in the state of Iowa, the Iowa State University (ISU) Medical Entomology Laboratory has conducted a surveillance program designed to monitor mosquito populations and mosquito-borne viruses in Iowa for the last 40 years. In 2007, three research farms administered by the ISU College of Agriculture and Life Sciences (Horticulture Station, Ames; McNay Farm, Chariton; and Western Farm, Castana) participated in our surveillance program, and this report summarizes their surveillance efforts

Injection of Double Stranded RNA Enhances Survival of Litopenaeus vannamei Challenged with White Spot Syndrome Virus

Double stranded RNA was synthesized in vitro with sequences corresponding to portions of the WSSV genome and were injected into shrimp 3 days prior to challenge with a lethal dose of WSSV

Direct Delivery of VP19 Double-Stranded RNA into Litopenaeus vannamei by Reverse Gavage Induces Protection against White Spot Syndrome Virus Disease

Double stranded RNA was synthesized in vitro and was delivered by reverse gavage (RG) compared to traditional intramuscular injection (IM) 3 days prior to challenge with a lethal dose of WSSV in both groups