Growth parameters analysed: D21 length (n = 48, F_(3,40) = 1.09, p = 0.3641), D21 weight (n = 48, F_(3,40) = 0.71, p = 0.5516), D21 condition factor (n = 48, F_(3,40) = 5.29, p = 0.0036), D42 length (n = 47, F_(3,39) = 1.30, p = 0.2884), D42 weight (n = 47, F_(3,39) = 0.90, p = 0.4500), and D42 condition factor (n = 47, F_(3,39) = 7.37, p = 0.0005).

<p>* = p<0.05,</p><p>** = p<0.01,</p><p>*** = p<0.001</p><p>Growth parameters analysed: D21 length (n = 48, F_(3,40) = 1.09, p = 0.3641), D21 weight (n = 48, F_(3,40) = 0.71, p = 0.5516), D21 condition factor (n = 48, F_(3,40) = 5.29, p = 0.0036), D42 length (n = 47, F_(3,39) = 1.30, p = 0.2884), D42 weight (n = 47, F_(3,39) = 0.90, p = 0.4500), and D42 condition factor (n = 47, F_(3,39) = 7.37, p = 0.0005).</p

Analyses of hypothalamic (<i>crf</i>) (n = 47, F_(3,39) = 9.71, p<0.0001), pituitary pars distalis (<i>pomc)</i> (n = 44, F_(3,36) = 20.83, p<0.0001), and interrenal (<i>star</i>) (n = 45, F_(3,37) = 7.75, p = 0.0004) genes (MNE) at day 42 of treatment.

<p>Error bars in scatter/boxplots are defined as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.g001" target="_blank">Fig 1</a>. For statistics, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.s003" target="_blank">S2 Table</a>.</p

Relationship between the hazard ratio (coxph regression model for a mean rater with state of the fish as response variable and the reflex index based on continuous reflex scores; see S13 Table) and the reflex impairment index based on continuous scores for plaice (<i>Pleuronectes platessa</i>) at 17, and 24 cm in total length.

<p>Relationship between the hazard ratio (coxph regression model for a mean rater with state of the fish as response variable and the reflex index based on continuous reflex scores; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179092#pone.0179092.s014" target="_blank">S13 Table</a>) and the reflex impairment index based on continuous scores for plaice (<i>Pleuronectes platessa</i>) at 17, and 24 cm in total length.</p

Cortisol (pmol per scale) in regenerated scales of 21 days (n = 48, F_(3,40) = 10.16, p<0.0001) (CCα = decision limit and CCβ = detection capability were calculated using an average weight of 0.026 g per scale).

<p>Error bars in scatter/boxplots are defined as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.g001" target="_blank">Fig 1</a>. For statistics, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.s003" target="_blank">S2 Table</a>.</p

Cortisol (pmol per scale) in ontogenetic scales after 21 and 42 days of treatment (CCα = decision limit and CCβ = detection capability were calculated using an average weight of 0.026 g per scale).

<p>One value (2.8 pmol per scale) of STRESS day 42 was omitted in the scatter/boxplot for reasons of presentation. Error bars in scatter/boxplots are defined as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.g001" target="_blank">Fig 1</a>. For statistics, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.s003" target="_blank">S2 Table</a>.</p

Optimized UPLC-MS/MS conditions per compound.

<p>* = quantifier ion</p><p>Optimized UPLC-MS/MS conditions per compound.</p

List of scoring criteria for categorical reflex responses (i.e., absent, weak, moderate, and strong) of plaice (<i>Pleuronectes platessa</i>) in the order tested within 5 s of observation after stimulus.

<p>List of scoring criteria for categorical reflex responses (i.e., absent, weak, moderate, and strong) of plaice (<i>Pleuronectes platessa</i>) in the order tested within 5 s of observation after stimulus.</p

Gene expression of <i>atp1a1a</i> in gills (MNE) (n = 47, F_(3,39) = 9.76, p<0.0001) after 42 days of treatment.

<p>Error bars in scatter/boxplots are defined as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.g001" target="_blank">Fig 1</a>. For statistics, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.s003" target="_blank">S2 Table</a>.</p

Regenerated scale morphometric analyses: area (n = 47, F_(3,39) = 13.22, p<0.0001), perimeter (n = 47, F_(3,39) = 5.65, p = 0.0026), circularity (n = 47, F_(3,39) = 0.36, p = 0.7812), and ridges (n = 47, F_(3,39) = 3.03, p = 0.0406).

<p>* = p<0.05,</p><p>** = p<0.01,</p><p>*** = p<0.001</p><p>Regenerated scale morphometric analyses: area (n = 47, F_(3,39) = 13.22, p<0.0001), perimeter (n = 47, F_(3,39) = 5.65, p = 0.0026), circularity (n = 47, F_(3,39) = 0.36, p = 0.7812), and ridges (n = 47, F_(3,39) = 3.03, p = 0.0406).</p

Gene expression of <i>col 1a1</i> in ontogenetic scale (MNE) (n = 47, F_(3,39) = 23.70, p<0.0001) after 42 days of treatment.

<p>Error bars in scatter/boxplots are defined as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.g001" target="_blank">Fig 1</a>. For statistics, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123411#pone.0123411.s003" target="_blank">S2 Table</a>.</p