13 research outputs found
The rapid interphase chromosome assay (RICA) implementation : comparison with other PCC methods
A report is presented on the advantages of the rapid interphase chromosome assay (RICA) and the
difficulties that may be met while implementing this method for application in biological dosimetry. The RICA
test can be applied on unstimulated human lymphocytes; this is an advantage in comparison with the dicentric
chromosomes or micronucleus tests. In the former two tests, stimulated lymphocytes are examined and hence,
48 h more are needed to obtain cells traversing the cell cycle. Due to the use of unstimulated nondividing cells,
higher numbers of cells are available for RICA analysis than for dicentric chromosomes or micronuclei tests.
Moreover, the method can be applied after exposure to ionizing radiation doses in excess of 5 Gy. Such doses
cause a signifi cant cell cycle delay or result in the loss of G2 phase and mitotic cells because of apoptosis. Therefore,
the traditional biodosimetry based on the evaluation of the incidence of damage to chromosomes is very
difficult to carry out. This is due to the lack of an adequate number of mitotic cells for analysis. RICA is free of
this disadvantage. An automatic microscope can be used to retrieve cell images; automatic image analysis can
also be used
The rapid interphase chromosome assay (RICA) implementation: comparison with other PCC methods
A report is presented on the advantages of the rapid interphase chromosome assay (RICA) and the
difficulties that may be met while implementing this method for application in biological dosimetry. The RICA
test can be applied on unstimulated human lymphocytes; this is an advantage in comparison with the dicentric
chromosomes or micronucleus tests. In the former two tests, stimulated lymphocytes are examined and hence,
48 h more are needed to obtain cells traversing the cell cycle. Due to the use of unstimulated nondividing cells,
higher numbers of cells are available for RICA analysis than for dicentric chromosomes or micronuclei tests.
Moreover, the method can be applied after exposure to ionizing radiation doses in excess of 5 Gy. Such doses
cause a signifi cant cell cycle delay or result in the loss of G2 phase and mitotic cells because of apoptosis. Therefore,
the traditional biodosimetry based on the evaluation of the incidence of damage to chromosomes is very
difficult to carry out. This is due to the lack of an adequate number of mitotic cells for analysis. RICA is free of
this disadvantage. An automatic microscope can be used to retrieve cell images; automatic image analysis can
also be used