Effect of cassava chips on quality of silage from fresh forage sorghum plus Cavalcade forage legume hay mixtures

Readers are free to share (to copy, distribute and transmit) and to remix (to adapt) the work under the conditions of Attribution, Noncommercial, and Share Alike (see http://creativecommons.org/licenses/by-nc-sa/3.0/)Two experiments investigated the effects on silage quality of adding cassava chips at a range of levels to a sorghum forage plus Cavalcade legume hay mixture at ensiling at the Experimental Farm, Khon Kaen University, Thailand. Cavalcade hay was 15% of chopped sorghum fresh weight and cassava chips were added at 0, 5, 10 and 15% of chopped sorghum fresh weight. The first experiment utilized sorghum and Cavalcade main crops and the second used the ratoon crop of sorghum and 2nd cut of Cavalcade. Dry matter percentage of ensiled mixtures and silages increased (P<0.05) with an increase in cassava chip levels in both experiments, but CP and NDF concentrations decreased (P<0.05). Dry matter degradability increased (P<0.05) with an increase in cassava chip levels up to 10% in both main and ratoon/2nd cut silages. Digestible energy and metabolizable energy of both silages increased significantly with an increase in cassava chip levels up to 10% for main crop and 15% for ratoon/2nd cut crops. While addition of cassava chips improved digestibility and energy content of silage, it lowered CP concentration. Use of fresh Cavalcade instead of hay should increase the CP levels and should be investigated along with animal feeding studies to test acceptance and animal performance.Peer reviewe

A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattle

A multiplex polymerase chain reaction (PCR) test was developed for identifying gastrointestinal (GI) nematodes that commonly infect cattle. This assay was developed using adult-derived genomic DNA and shown capable of discriminating parasite eggs from the feces of experimentally-infected animals at both the species and genus levels. Sequence data from internal (ITS) and external (ETS) transcribed spacers of the ribosomal DNA (rDNA) repeats as well as the 3′-end of the small subunit rDNA and 5′-end of the large subunit rDNA were used to generate five primer sets which, when used simultaneously in a multiplex PCR, produce a unique electrophoretic DNA banding pattern charac- terized by a single DNA fragment for Ostertagia ostertagi (257 bp), Haemonchus placei (176 bp),Oesophagostomum radiatum (329 bp), Trichostrongylus colubriformis (243 bp) and Cooperia on- cophora (151 bp). In a similar manner, the constructed primer sets amplified DNA from Ostertagia lyrata, Haemonchus contortus, Trichostrongylus axei, Cooperia surnabada and Cooperia punctata. With respect to H. contortus, a closely migrating doublet was generated suggesting size heterogene- ity in the ETS which is consistent with multiple rDNA repeat units within this species. PCR analyses using mixtures of monospecifically-purified nematode eggs indicated a sensitivity of less than 0.5 egg-DNA equivalent per species. Although, not designed as a quantitative technique, relative PCR signal intensities corresponded to relative egg burdens within the DNA samples from mixed species of eggs. Published by Elsevier Science B.V

Three-dimensional images of choanoflagellate loricae

Choanoflagellates are unicellular filter-feeding protozoa distributed universally in aquatic habitats. Cells are ovoid in shape with a single anterior flagellum encircled by a funnel-shaped collar of microvilli. Movement of the flagellum creates water currents from which food particles are entrapped on the outer surface of the collar and ingested by pseudopodia. One group of marine choanoflagellates has evolved an elaborate basket-like exoskeleton, the lorica, comprising two layers of siliceous costae made up of costal strips. A computer graphic model has been developed for generating three-dimensional images of choanoflagellate loricae based on a universal set of 'rules' derived from electron microscopical observations. This model has proved seminal in understanding how complex costal patterns can be assembled in a single continuous movement. The lorica, which provides a rigid framework around the cell, is multifunctional. It resists the locomotory forces generated by flagellar movement, directs and enhances water flow over the collar and, for planktonic species, contributes towards maintaining cells in suspension. Since the functional morphology of choanoflagellate cells is so effective and has been highly conserved within the group, the ecological and evolutionary radiation of choanoflagellates is almost entirely dependent on the ability of the external coverings, particularly the lorica, to diversify

Optimal Remote State Preparation

We prove that it is possible to remotely prepare an ensemble of non-commuting mixed states using communication equal to the Holevo information for this ensemble. This remote preparation scheme may be used to convert between different ensembles of mixed states in an asymptotically lossless way, analogous to concentration and dilution for entanglement.Comment: 4 pages, no figure

Geometric phase for an adiabatically evolving open quantum system

We derive an elegant solution for a two-level system evolving adiabatically under the influence of a driving field with a time-dependent phase, which includes open system effects such as dephasing and spontaneous emission. This solution, which is obtained by working in the representation corresponding to the eigenstates of the time-dependent Hermitian Hamiltonian, enables the dynamic and geometric phases of the evolving density matrix to be separated and relatively easily calculated.Comment: 10 pages, 0 figure

Quasi-local contribution to the scalar self-force: Geodesic Motion

We consider a scalar charge travelling in a curved background spacetime. We calculate the quasi-local contribution to the scalar self-force experienced by such a particle following a geodesic in a general spacetime. We also show that if we assume a massless field and a vacuum background spacetime, the expression for the self-force simplifies significantly. We consider some specific cases whose gravitational analog are of immediate physical interest for the calculation of radiation reaction corrected orbits of binary black hole systems. These systems are expected to be detectable by the LISA space based gravitational wave observatory. We also investigate how alternate techniques may be employed in some specific cases and use these as a check on our own results.Comment: Final Phys. Rev. D version. 24 pages, revtex4. Minor typos correcte

Quantum walks on circles in phase space via superconducting circuit quantum electrodynamics

We show how a quantum walk can be implemented for the first time in a quantum quincunx created via superconducting circuit quantum electrodynamics (QED), and how interpolation from quantum to random walk is implemented by controllable decoherence using a two resonator system. Direct control over the coin qubit is difficult to achieve in either cavity or circuit QED, but we show that a Hadamard coin flip can be effected via direct driving of the cavity, with the result that the walker jumps between circles in phase space but still exhibits quantum walk behavior over 15 steps.Comment: 8 pages, 4 figures, 2 table

Monogamy and polygamy for multi-qubit entanglement using R\'enyi entropy

Using R\'enyi-$\alpha$ entropy to quantify bipartite entanglement, we prove monogamy of entanglement in multi-qubit systems for $\alpha \geq 2$. We also conjecture a polygamy inequality of multi-qubit entanglement with strong numerical evidence for $0.83-\epsilon \leq \alpha \leq 1.43+\epsilon$ with $0<\epsilon<0.01$.Comment: 19 pages, 2 figure

Rumen function and digestion parameters associated with differences between sheep in methane emissions when fed chaffed lucerne hay

An indoor experiment involving 10 rumen-cannulated Romney sheep was conducted in May and June 1998 at AgResearch Grasslands, Palmerston North, New Zealand, under restricted feeding conditions. in order to test the hypothesis that animal factors, in particular rumen fractional outflow rate (FOR) and rumen volume, have an influence on the between-sheep variation in methane (CH4) emission. Sheep were fed 2-hourly on chaffed lucerne hay. Following an acclimatization period of 21 days, the experiment lasted 16 days. Energy and nitrogen (N) balances were measured on days 1-6. Cr-EDTA marker was continuously infused into the rumen from day 9 to 16, and rumen contents emptied and sampled on days 13 and 16. Particulate and fluid FOR were estimated using feed lignin and Cr-EDTA, respectively. Daily CH, production was measured by the sulphur hexafluoride tracer technique on days 2, 5, 6, 12 and 15 of the experiment. CH4 production (g/day) was positively correlated with the pool size of organic matter (OM) in the rumen (OM pool, g) (r = 0.84, P = 0.002), OM intake (OMI, g/day) (r = 0.67, P = 0.04), and the rumen fill (g. wet digesta) (r = 0.76, P = 0.01). Multiple regression analysis showed that CH4 production was best predicted (R-2 = 0.88) as a function of OM pool and the molar % of butyrate; however, OM pool alone accounted for a large proportion (R-2 = 0.71) of the variation in CH4 production. CH4 yield (% gross energy intake, % GEI) was negatively correlated with the particulate FOR (%/h) ( r= -0.75, P = 0.01) and buffering capacity of rumen fluid (mmol HCl) (r = -0.72, P = 0.02) but positively correlated with the digestibility of cellulose (r = 0.66, P = 0.04). Multiple regression analysis showed that CH, yield was best predicted as a function of particulate FOR, OMI (g/kg liveweight(0.75)) and the molar % of butyrate (R-2 = 0.88). Particulate FOR alone explained a large proportion (R-2 = 0.57) of the variation in CH4 Yield. Particulate FOR was negatively correlated with rumen fill (r = -0.69, P = 0.03) and digestibility of cellulose (r = -0.65, P = 0.04). These results suggest that sheep with lower rumen particulate FOR (i.e. longer rumen retention times) had larger rumen fills and higher fibre digestibilities and CH4 yields. If rumen particulate FOR is to be used as a tool for CH4 mitigation, the repeatability of its relationship to CH4 emission must be assessed, preferably under grazing conditions