The State of the Region: Hampton Roads 2006

This is Old Dominion University\u27s seventh annual State of the Region report. While it represents the work of many people connected in various ways to the university, the report does not constitute an official viewpoint of Old Dominion, or it\u27s president, Roseann Runte. The report maintains the goal of stimulating thought and discussion that ultimately will make Hampton Roads an even better place to live. We are proud of our region\u27s many successes, but realize that it is possible to improve our performance. In order to do so, we must have accurate information about where we are and a sound understanding of the policy options open to us.https://digitalcommons.odu.edu/economics_books/1012/thumbnail.jp

Fly Light Split-GAL4 Driver Collection

<p>The data presented on this site are the work of the <a href="http://janelia.org/team-project/fly-light" target="_blank">Janelia FlyLight Project Team</a> and the laboratories of <a href="http://www.janelia.org/lab/rubin-lab" target="_blank">Gerald M. Rubin</a>. </p><p>The split-GAL4 lines can be requested from the Janelia fly facility by performing a search and adding the desired lines to your cart. You will then be able to use the FlyBank website to tell us where to send them. For additional help ordering lines, please contact us at <a href="mailto:flybank.janelia.org">flybank.janelia.org</a></p><p>In publications, please attribute the data presented on this site to one of the following papers, as follows: <br><br>For the overall strategy and methods used to produce the split-GAL4 lines for the mushroom body neurons: <br>Aso, Y., Hattori, D., Yu, Y., Johnston, R. M., Iyer, N., Ngo, T. B., Dionne, H., Abbott, L. F., Axel, R., Tanimoto, H. & Rubin, G. M. . The neuronal architecture of the mushroom body provides a logic for associative learning. <a href="http://elifesciences.org/content/3/e04577" target="_blank">eLife (2014) 3:e04577</a><br><br>For split-GAL4 lines for the Lobula Columnar (LC) visual projection neurons:<br>Wu, M., Nern, A., Williamson, W. R., Morimoto, M. M., Reiser, M. B., Card, G. M. & Rubin, G. M. Visual projection neurons in the Drosophila lobula link feature detection to distinct behavioral programs. under review<br><br>For refinement of the split-GAL4 vectors and methodology: <br>Pfeiffer, B. D., Ngo, T. T., Hibbard, K. L., Murphy, C., Jenett, A., Truman, J. W. & Rubin, G. M. Refinement of tools for targeted gene expression in Drosophila. <a href="http://www.genetics.org/content/186/2/735.long" target="_blank">Genetics (2010) 186: 735-55</a>. <br><br>For Multicolor Flp-out (MCFO) technique and single cell labeling:<br>Nern, A., Pfeiffer, B.D., and Rubin, G.M. Optimized tools for multicolor stochastic labeling reveal diverse stereotyped cell arrangements in the fly visual system. <a href="http://www.pnas.org/content/112/22/E2967.long" target="_blank">Proc Natl Acad Sci USA (2015) 112: E2967-2976</a>. <br><br>Split-GAL4 lines were designed based on the expression patterns of GAL4 driver lines in the adult nervous system: <br>The Janelia collection of lines is described in Jenett, A., Rubin, G.M., Ngo, T.-T. B., Shepherd, D., Murphy, C., Dionne, H., Pfeiffer, B.D., Cavallaro, A., Hall, D., Jeter, J., Iyer, N., Fetter, D., Hausenfluck, J.H., Peng, H., Trautman, E., Svirskas, R., Myers, G.W., Iwinski, Z.R., Aso, Y., DePasquale, G.M., Enos, A., Hulamm, P., Lam, S.C.B., Li, H-H., Laverty, T., Long, F., Qu, L., Murphy, S.D., Rokicki, K., Safford, T., Shaw, K., Simpson, J.H., Sowell, A., Tae, S., Yu, Y., Zugates, C.T. A GAL4-Driver Line Resource for Drosophila Neurobiology. <a href="http://www.cell.com/cell-reports/fulltext/S2211-1247(12)00292-6" target="_blank">Cell Reports (2012) 2: 991-1001</a> <br><br>The VT collection of lines is described in Kvon, E.Z., Kazmar, T., Stampfel, G., Yanez-Cuna, J.O., Pagani, M., Schernhuber, K., Dickson, B.J., and Stark, A. Genome-scale functional characterization of Drosophila developmental enhancers in vivo. <a href="http://www.nature.com/nature/journal/vaop/ncurrent/full/nature13395.html" target="_blank">Nature (2014) 512: 91-95</a> and Barry J. Dickson, unpublished data. <br><br>For opening and viewing h5j and LSM stacks:<br>Use <a href="http://fiji.sc/" target="_blank">Fiji</a> (<a href="http://fiji.sc/" target="_blank">http://fiji.sc</a>). Fiji has a built-in plugin (H5J_Loader_Plugin-1.0.4) for opening stack in h5j format, a "visually lossless" compression format.</p